Log In Sign up
The largest database of trusted experimental protocols

Recombinant mouse il 22

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

Recombinant mouse IL-22 is a laboratory reagent that is used in research applications. It is a cytokine protein produced through recombinant DNA technology. The core function of this product is to serve as a tool for scientific investigation and experimentation.

Automatically generated - may contain errors

Lab products found in correlation

B27 supplement, by Thermo Fisher Scientific (2 mentions) Lt α1β2, by R&D Systems (1 mentions) Advanced dmem f12, by Thermo Fisher Scientific (1 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions) Noggin, by Thermo Fisher Scientific (1 mentions) N2 supplement, by Thermo Fisher Scientific (1 mentions) N acetylcysteine, by Merck Group (1 mentions) Matrigel, by Corning (1 mentions) Invivopure dilution buffer, by BioXCell (1 mentions)

Close spelling variants of this product

Recombinant IL-22 IL-22

4 protocols using recombinant mouse il 22

1

Modeling M-Cell Differentiation in Intestinal Organoids

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Proximal small intestine was incubated with 2 mM EDTA/PBS for 30 min on ice. Then, intestinal crypts were isolated by vigorous pipetting and embedded in Matrigel (Corning). Crypts were cultured in Advanced DMEM/F12 (Thermo Fisher Scientific) containing 10 ng/ml EGF (PeproTech), R-spondin1 conditioning medium (provided by C.J. Kuo; Ootani et al., 2009 (link)), 100 ng/ml Noggin (PeproTech), B27 supplement (Thermo Fisher Scientific), N2 supplement (Thermo Fisher Scientific), and 1 mM N-acetylcysteine (Sigma-Aldrich). Recombinant glutathione S-transferase–mouse RANKL fusion protein (GST-RANKL; 500 ng/ml; Knoop et al., 2009 (link)) and LT α1β2 (1 µg/ml; R&D Systems) were used for the stimulation of organoids. Recombinant mouse IL-22 (100 ng/ml; PeproTech) was added into RANKL-stimulated organoids to evaluate the effect of IL-22 signaling on M cell differentiation.
Jinnohara T., Kanaya T., Hase K., Sakakibara S., Kato T., Tachibana N., Sasaki T., Hashimoto Y., Sato T., Watarai H., Kunisawa J., Shibata N., Williams I.R., Kiyono H, & Ohno H. (2017). IL-22BP dictates characteristics of Peyer’s patch follicle-associated epithelium for antigen uptake. The Journal of Experimental Medicine, 214(6), 1607-1618.
+ Open protocol
+ Expand
2

Recombinant IL-22 Injection for Post-MI

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant mouse IL-22 (210–22; Peprotech EC Ltd., Rocky Hill, NJ) was dissolved in 1% bovine serum albumin (BSA) in phosphate-buffered saline (PBS). IL-22KO mice were subcutaneously injected with Recombinant mouse IL-22 (100 ng/g) or an equal volume of 1% BSA in PBS twice daily for 7 days after MI.
Yamamoto M., Yasukawa H., Takahashi J., Nohara S., Sasaki T., Shibao K., Akagaki D., Okabe K., Yanai T., Shibata T, & Fukumoto Y. (2023). Endogenous interleukin-22 prevents cardiac rupture after myocardial infarction in mice. PLOS ONE, 18(6), e0286907.
+ Open protocol
+ Expand
3

Rapid Systemic IL-22 Administration

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant mouse IL-22 (3 µg/mouse; PeproTech) was intravenously injected to mice. After 15 min, mice were sacrificed and PPs were collected. The PPs were fixed and embedded in paraffin blocks for sectioning.
Jinnohara T., Kanaya T., Hase K., Sakakibara S., Kato T., Tachibana N., Sasaki T., Hashimoto Y., Sato T., Watarai H., Kunisawa J., Shibata N., Williams I.R., Kiyono H, & Ohno H. (2017). IL-22BP dictates characteristics of Peyer’s patch follicle-associated epithelium for antigen uptake. The Journal of Experimental Medicine, 214(6), 1607-1618.
+ Open protocol
+ Expand
4

Therapeutic Supplementation of IL-22 in Bone Repair

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were osteotomized as described above, and treatment was started 24 h post-surgery to mimic the feasibility of this treatment approach under clinical conditions. The supplementation and neutralization of IL-22 were performed via intraperitoneal injection of recombinant mouse IL-22 (PeproTech, Rocky Hill, NJ, USA) or rat anti-mouse functional grade IL-22 monoclonal antibody (clone IL22JOP, Thermo Fisher, Waltham, MA, USA). IL-22 supplementation was performed with 5 µg protein in 100 µl InVivoPure dilution buffer (BioXCell, Lebanon, NH, USA) every second day, with the last injection timepoint 1 week post-surgery. The neutralizing IL-22 antibody was diluted to 100 µg in 100 µl InVivoPure dilution buffer, with a treatment schedule similar to that described for IL-22 supplementation.
Bucher C.H., Berkmann J.C., Burkhardt L.M., Paschke C., Schlundt C., Lang A., Wolter A., Damerau A., Geissler S., Volk H.D., Duda G.N, & Schmidt-Bleek K. (2022). Local immune cell contributions to fracture healing in aged individuals – A novel role for interleukin 22. Experimental & Molecular Medicine, 54(8), 1262-1276.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!