Muscle force measurement in regenerating TA muscle was performed in situ using a 1300A Whole‐Animal System (Aurora Scientific) as described previously (You, Singh, et al., 2021 (link)). Briefly, the mouse was stabilized on an isothermal stage set at 38°C by inserting a needle through a fixed post and patella tendon. The distal tendon of the TA muscle was connected to the lever arm of the force transducer through a 3‐0 suture line. The muscle was directly stimulated through two electrodes with 0.2‐ms square‐wave pulses at 0.2 mA and adjusted to optimal muscle length where maximal twitch force was produced. The maximum isometric tetanic force was determined in the frequency range of 50–200 Hz with a 300‐ms pulse duration, with each contraction separated by a 1‐minute rest. Throughout the experiments, the exposed TA muscle was kept moist with a warm PBS‐soaked KimWipe.
1300a whole animal system
Manufactured by Aurora Scientific
Sourced in Canada
The 1300A Whole Animal System is a laboratory instrument designed for the comprehensive analysis of physiological parameters in small animals. It provides a platform for researchers to monitor and record various biological functions, including cardiovascular, respiratory, and behavioral data. The 1300A is equipped with the necessary hardware and software to facilitate seamless data acquisition and analysis, enabling researchers to gain valuable insights from their animal studies.
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Lab products found in correlation
7 protocols using 1300a whole animal system
1
In Situ Muscle Force Measurement
2
In situ Muscle Force Measurement
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In situ force measurements of TA muscles were conducted as done previously43 ,44 , and the data were analyzed using the 1300A Whole Animal System (Aurora Scientific). Mice were anaesthetized with isoflurane and placed on isothermal stage. Intact TA muscles were dissected and constantly immersed in Ringer’s solution. Single twitch or tetanic contractions were elicited with electrical stimulations applied by two electrodes placed on either side of the muscle. In all experiments we used 0.2 ms pulses at 10 V supramaximal voltage. Muscle optimal length (Lo) that allows a maximum isometric twitch force (Pt) was determined by a series of twitch contractions with small variations of the muscle tension. To obtain maximum isometric tetanic force (Po), muscles were stimulated for 300 ms at different frequencies from 50 to 200 Hz. A 1 min recovery period was allowed between stimulations. The muscles were then fatigued at 150 Hz with one contraction per second for 180 s. Muscle wet weight and Lo were used to calculate the cross-sectional area (CSA) of the TA muscle for normalization to obtain specific isometric twitch force sPt (kN/mm2) and sPo (kN/mm2).
3
In Situ Muscle Force Measurements
4
In Situ Muscle Force Measurements
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In situ force measurements of TA muscles were conducted as previously
described45 ,46 , and the data were analyzed using the
1300A Whole Animal System (Aurora Scientific) using ASI 610A Dynamic Muscle
Control v5.420 software. Mice were anaesthetized with isoflurane and placed on
isothermal stage. Intact TA muscles were dissected and constantly immersed in
Ringer’s solution (homemade). Single twitch or tetanic contractions were
elicited with electrical stimulations applied by two electrodes placed on either
side of the muscle. In all experiments 0.2 ms pulses at 10 V supramaximal
voltage were used. Muscle optimal length (Lo) that allows a maximum isometric
twitch force (Pt) was determined by a series of twitch
contractions with small variations of the muscle tension. To obtain maximum
isometric tetanic force (Po), muscles were stimulated for 300
ms at different frequencies from 50 to 200 Hz. A 1 min recovery period was
allowed between stimulations. Muscle wet weight and Lo were used to calculate
the cross-sectional area (CSA) of the TA muscle for normalization to obtain
specific isometric twitch force sPt (kN/mm2) and
sPo (kN/mm2).
described45 ,46 , and the data were analyzed using the
1300A Whole Animal System (Aurora Scientific) using ASI 610A Dynamic Muscle
Control v5.420 software. Mice were anaesthetized with isoflurane and placed on
isothermal stage. Intact TA muscles were dissected and constantly immersed in
Ringer’s solution (homemade). Single twitch or tetanic contractions were
elicited with electrical stimulations applied by two electrodes placed on either
side of the muscle. In all experiments 0.2 ms pulses at 10 V supramaximal
voltage were used. Muscle optimal length (Lo) that allows a maximum isometric
twitch force (Pt) was determined by a series of twitch
contractions with small variations of the muscle tension. To obtain maximum
isometric tetanic force (Po), muscles were stimulated for 300
ms at different frequencies from 50 to 200 Hz. A 1 min recovery period was
allowed between stimulations. Muscle wet weight and Lo were used to calculate
the cross-sectional area (CSA) of the TA muscle for normalization to obtain
specific isometric twitch force sPt (kN/mm2) and
sPo (kN/mm2).
5
In Vivo Muscle Force Assessment
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Prior to euthanasia, in vivo muscle force testing was conducted on the tibialis anterior muscles using the 1300A Whole Animal System (Aurora Scientific, Canada). Animals were sedated (2–4% isoflurane/400 mL/min O2), and placed in a supine position on the platform. Each leg was immobilized at the knee using a screw-clamp and the paw was pressed firmly onto a pedal and secured with surgical tape. Two electrodes were inserted subcutaneously above the TA muscles to deliver electrical impulses for twitch and tetanic contractions. The foot flexes the pedal upon stimulation to produce a force–time curve. Twitch testing was performed 3 times using a 0.2 ms pulse width and the average of three test was reported. Tetany testing was performed 3 times at 140 Hz with a 500 m-sec stimulation duration until full muscle recruitment was reached followed by 120 s rest periods between stimulations to allow for muscle recovery. The average of three tests was reported. Muscle force testing was performed on all animals.
6
In situ Muscle Force Analysis
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Muscle force analyses and eccentric contractions were performed in situ using a 1300A Whole‐Animal System (Aurora Scientific) as described previously.12 Briefly, the distal tendon of the TA muscle was connected to the lever arm of the force transducer through a 3–0 suture line. The muscle was electrically stimulated with 0.2‐ms square‐wave pulses at 0.2 mA and adjusted to optimal muscle length where maximal twitch force was produced. The maximum isometric tetanic force was determined in the frequency range of 50–200 Hz with 300‐ms pulse duration, with each contraction separated by a 1‐minute rest. For eccentric contractions, the TA muscle was lengthened to 1.2 fibre length (0.6 × optimal muscle length) at a velocity of 1.5 fibre length/s and held for 200 ms before being returned to its optimal length at the same velocity. The muscle was stimulated 100 ms prior to and during the lengthening period at 100 Hz. Specific isometric twitch/tetanic force was calculated by dividing maximal isometric twitch/tetanic force by physiological cross‐sectional area [muscle mass/(fibre length × muscle density 1.06 g/cm3)].
7
In situ Muscle Force Measurement
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