Apc conjugated anti mouse st2 ab

Reagents used in this study were acquired from the indicated suppliers: SR9009 (Merck Millipore, Burlington, MA, USA); SR9011, GSK4112, LPS, and Evans blue (Sigma-Aldrich, St. Louis, MO, USA); recombinant mouse IL-3 (PeproTech, Rocky Hill, NJ, USA); recombinant mouse IL-33 (R & D Systems, Minneapolis, MN, USA); anti-TNP IgE, anti-DNP mouse IgE mAb, anti-mouse CD16/32, PE-conjugated anti-mouse c-kit Ab, and APC-conjugated anti-mouse ST2 Ab (BD Bioscience, San Jose, CA, USA); DNP-BSA (Cosmo Bio, Tokyo, Japan); APC-conjugated anti-mouse CD63 Ab, FITC-conjugated anti-mouse FcεRIα (BioLegend, San Diego, CA, USA); anti-phospho-NF-κB p65 Ab (Ser536; #3033), anti-phospho-p38 MAPK Ab (Thr180/Thy182; #4511), anti-phospho-Gab2 Ab (Tyr452; #3882), anti-phosph-PI3 Kinase p85 (Tyr458)/p55 (Tur199) Ab (#4228), and anti-β-actin Ab (#4970) (Cell Signaling Technology, Danvers, MA, USA).

Reagents used in this study were acquired from the indicated suppliers: trans-resveratrol, cyclodextrin, 5Z-7-Oxozeaenol and Evans blue (Sigma-Aldrich, St. Louis, MO, USA); LY294002, wortmannin, and ICI 182,780 (Abcam, Cambridge, UK); PF-3644022 (TOCRIS Bioscience, Bristol, U.K.); recombinant mouse IL-3, recombinant mouse stem cell factor (SCF), and recombinant human IL-33 (PeproTech, Rocky Hill, NJ, USA); recombinant human IL-3 (Thermo Fisher Scientific, Wilmington, DE, USA); recombinant mouse IL-33 (R & D systems, Minneapolis, MN, USA); anti-TNP IgE, anti–DNP mouse IgE mAb, anti–mouse CD16/32, PE–conjugated anti–mouse c-kit Ab, and APC–conjugated anti–mouse ST2 Ab (BD Bioscience, San Jose, CA, USA); DNP–BSA (Cosmo Bio, Tokyo, Japan); APC–conjugated anti–mouse CD63 Ab (Miltenyi Biotec, Bergisch Gladbach, Germany); anti–phospho–transforming growth factor β-activated kinase 1 (TAK1) Ab (Thr184/187; #4508), anti–phospho–IκB kinase (IKK) α/β Ab (Ser176/177; #2078), anti–phospho–p65 Ab (Ser536; #3033), anti–phospho–p38 Ab (Thr180/Thy182; #4511), anti–phospho–MK2 Ab (Thr334; #3007), anti–phospho–Akt Ab (Ser473; #4060), anti–phospho–Gab2 Ab (Tyr452; #3882), anti–phospho–Syk Ab (Tyr525/526; #2710), anti–phospho–p70S6K Ab (Tyr389; #9234), anti–phospho–AMPK Ab (Thr172; #2535), anti–β-actin Ab (#4970), and AICAR (#9944) (Cell Signaling Technology, Danvers, MA, USA).