Nutrient agar, de Mann–Rogosa and Sharpe (MRS) broth, peptone water, and glucose were obtained from PANREAC (Castellar del Vallès, Barcelona). Ethanol, xylene, and phosphate buffered saline were obtained from Merck (Darmstadt, Germany). MacConkey agar, pepsin, pancreatin, bile salts, and aniline blue were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Nutrient agar
Manufactured by ITW Reagents
Sourced in Spain
Nutrient agar is a solid culture medium used to cultivate a wide range of microorganisms. It provides essential nutrients and a suitable growth environment for the propagation of bacteria, fungi, and other microbes.
Automatically generated - may contain errors
Lab products found in correlation
Glucose, by ITW Reagents (1 mentions)
Aniline blue, by Merck Group (1 mentions)
Bile salt, by Merck Group (1 mentions)
Macconkey agar, by Merck Group (1 mentions)
Pepsin, by Merck Group (1 mentions)
Pancreatin, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Xylene, by Merck Group (1 mentions)
Crystal violet, by ITW Reagents (1 mentions)
Methanol, by ITW Reagents (1 mentions)
Xylose lysine deoxycholate (xld), by Thermo Fisher Scientific (1 mentions)
2 protocols using nutrient agar
1
Bacterial Culture Media Preparation
2
Bacterial Inoculum Preparation and Biofilm Inhibition
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Muller-Hinton Broth (Panreac Applichem, Barcelona, Spain) and Nutrient Agar (Panreac Applichem, Barcelona, Spain) were used to revitalize and prepare the bacterial inocula. Species confirmation was performed on selective medium XLD (Oxoid, Hampshire, UK) and CHROMID SM2 (Biomérieux, Marcy-l’Étoile, France). Serial dilutions were performed with 0.85% saline solution (Sodium Chloride 99.85%, Acros Organics). Crystal Violet (Panreac Applichem, Barcelona, Spain) and Methanol (EMSURE ACS, ISO, Reag. Ph. Eur., Merck, Kenilworth, NJ, USA) were used for Biofilm Inhibitory Concentration (BIC) tests, respectively, to stain the bacterial cells and solubilize dye bound in each well of the microplate.
The derivative 12-mer peptide 1018-K6 was purchased from SynPeptide Co., LTD (Shanghai, China).
The derivative 12-mer peptide 1018-K6 was purchased from SynPeptide Co., LTD (Shanghai, China).
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