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Anti tfam

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Anti-TFAM is a laboratory reagent that binds to and inhibits the transcription factor A, mitochondrial (TFAM) protein. TFAM is a key regulator of mitochondrial DNA transcription and replication. Anti-TFAM can be used in research applications to investigate the role of TFAM in cellular processes.

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Lab products found in correlation

Anti coxii, by Abcam (1 mentions) Anti vinculin, by Thermo Fisher Scientific (1 mentions) Anti gapdh, by Merck Group (1 mentions) Anti ucp1, by Merck Group (1 mentions) Anti phospho ampkα, by Cell Signaling Technology (1 mentions) Anti ampkα, by Cell Signaling Technology (1 mentions) Anti pgc 1α, by Merck Group (1 mentions) Anti cat, by Merck Group (1 mentions) Anti β actin, by Merck Group (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Bradford assay reagent, by Bio-Rad (1 mentions) Protein g sepharose, by Santa Cruz Biotechnology (1 mentions) O 2 acetamido 2 deoxy d glucopyranosylidene amino n phenylcarbamate pugnac, by LGC (1 mentions) Tween 20, by Merck Group (1 mentions) D glucose, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Omega Scientific (1 mentions)

3 protocols using anti tfam

1

Mitochondrial Protein Analysis Protocol

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The following antibodies were used for this study: anti-Vinculin (Invitrogen, # 700,062), OxPhos Human WB Antibody Cocktail (Invitrogen, # 45-8199), OxPhos Rodent WB Antibody Cocktail (Invitrogen, #45-8099), anti-TFAM (Sigma-Aldrich, #ABE483), anti-GAPDH (Sigma-Aldrich, #G9545), anti-COXII (Abcam, #ab198286), MTCO1 Monoclonal Antibody (Invitrogen, # 459,600), goat anti-mouse IgG (H + L) HRP conjugated (Jackson ImmunoResearch), and goat anti-rabbit IgG Antibody, (H + L) HRP conjugated (Jackson ImmunoResearch).
DeFoor N., Paul S., Li S., Basso E.K., Stevenson V., Browning J.L., Prater A.K., Brindley S., Tao G, & Pickrell A.M. (2023). Remdesivir increases mtDNA copy number causing mild alterations to oxidative phosphorylation. Scientific Reports, 13, 15339.
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2

Western Blot Analysis of Metabolic Markers

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Muscles and brown fat were collected and frozen immediately. Whole-cell lysates were prepared in a lysis buffer by sonications as described previously [25 (link)]. The antibodies used and their sources were as follows: anti-phospho-AMPKα (Thr 172), anti-AMPKα (Cell Signaling Technology, Danvers, MA, USA), anti-PGC1α, anti-NRF1, anti-TFAM, anti-UCP1, anti-HO-1, anti-superoxide dismutase (SOD), anti-CAT, and anti-β-actin (Sigma-Aldrich, St. Louis, MO, USA). Bands were developed using Western detection reagent (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA) and quantified by densitometry using ImageJ.
Song M.Y., Lim S.K., Wang J.H, & Kim H. (2018). The Root of Atractylodes macrocephala Koidzumi Prevents Obesity and Glucose Intolerance and Increases Energy Metabolism in Mice. International Journal of Molecular Sciences, 19(1), 278.
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3

HCAEC Growth Media Preparation

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HCAEC growth media was from Cell Applications, Inc. Normal-glucose (NG, 5 mM D-glucose) Dulbecco’s Modified Eagle Medium (DMEM), HG (25 mM D-glucose) DMEM, trypsin and antibiotics were from GIBCO-BRL Invitrogen. Fetal bovine serum (FBS) was from Omega Scientific, and bovine serum albumin (BSA), Na3VO4, NaF, Tween-20 and D-glucose were from Sigma-Aldrich. Polyvinylidene difluoride (PVDF) membranes were from Immobilon and Bradford assay reagent was from Bio-Rad. Epi, protease and phosphatase inhibitor cocktail (P8340, P0044 and P2850), NG-nitro-L-arginine methyl ester hydrochloride (L-NAME), D-glucose and anti-TFAM were obtained from Sigma-Aldrich. O-(2-acetamido-2-deoxy-D-glucopyranosylidene) amino N phenylcarbamate (PugNac) was purchased from Toronto Research Chemicals. Protein-G-sepharose was from Santa Cruz Biotechnologies.
Ramírez-Sánchez I., Rodríguez A., Moreno-Ulloa A., Ceballos G, & Villarreal F. (2016). (−)-Epicatechin-induced recovery of mitochondria from simulated diabetes: Potential role of endothelial nitric oxide synthase. Diabetes & vascular disease research, 13(3), 201-210.
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