Log In Sign up
The largest database of trusted experimental protocols

β lactamase ccf4 assay

Manufactured by Thermo Fisher Scientific
Visit Supplier

The β-lactamase-CCF4 assay is a laboratory tool used to detect and measure the presence of β-lactamase, an enzyme that confers antibiotic resistance. The assay utilizes the fluorescent substrate CCF4 to report the activity of β-lactamase in a sample.

Automatically generated - may contain errors

Lab products found in correlation

Probenicid, by Merck Group (3 mentions)

3 protocols using β lactamase ccf4 assay

1

Quantification of Vacuolar Escape

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Quantification of vacuolar escape using the β-lactamase/CCF4 assay (Life technologies) was performed as previously described [33 (link)]. Briefly, bone marrow-derived macrophages seeded onto non-treated plates were infected as described above for 2 h, washed and incubated in CCF4 for 1 h at room temperature in the presence of 2.5 mM probenicid (Sigma). Propidium iodide negative cells were considered for the quantification of cells containing cytosolic F. novicida using excitation at 405 nm and detection at 450 nm (cleaved CCF4) or 510 nm (intact CCF4).
Rigard M., Bröms J.E., Mosnier A., Hologne M., Martin A., Lindgren L., Punginelli C., Lays C., Walker O., Charbit A., Telouk P., Conlan W., Terradot L., Sjöstedt A, & Henry T. (2016). Francisella tularensis IglG Belongs to a Novel Family of PAAR-Like T6SS Proteins and Harbors a Unique N-terminal Extension Required for Virulence. PLoS Pathogens, 12(9), e1005821.
+ Open protocol
+ Expand
2

Quantifying Cytosolic Bacterial Escape

Check if the same lab product or an alternative is used in the 5 most similar protocols
Quantification of vacuolar escape using the β-lactamase-CCF4 assay (Life technologies) was performed following manufacturer’s instructions. Briefly, macrophages seeded onto non-treated plates were infected as previously described for 1 h, washed and incubated in CCF4 for 1 hour at room temperature in the presence of 2.5 mM probenicid (Sigma). Propidium iodide negative cells were considered for the quantification of cells containing cytosolic F. novicida using excitation at 405 nm and detection at 450 nm (cleaved CCF4) or 510 nm (intact CCF4).
Meunier E., Wallet P., Dreier R.F., Costanzo S., Anton L., Rühl S., Dussurgey S., Dick M.S., Kistner A., Rigard M., Degrandi D., Pfeffer K., Yamamoto M., Henry T, & Broz P. (2015). Guanylate-binding proteins promote AIM2 inflammasome activation during Francisella novicida infection by inducing cytosolic bacteriolysis and DNA release. Nature immunology, 16(5), 476-484.
+ Open protocol
+ Expand
3

Quantifying Cytosolic Bacterial Escape

Check if the same lab product or an alternative is used in the 5 most similar protocols
Quantification of vacuolar escape using the β-lactamase-CCF4 assay (Life technologies) was performed following manufacturer’s instructions. Briefly, macrophages seeded onto non-treated plates were infected as previously described for 1 h, washed and incubated in CCF4 for 1 hour at room temperature in the presence of 2.5 mM probenicid (Sigma). Propidium iodide negative cells were considered for the quantification of cells containing cytosolic F. novicida using excitation at 405 nm and detection at 450 nm (cleaved CCF4) or 510 nm (intact CCF4).
Meunier E., Wallet P., Dreier R.F., Costanzo S., Anton L., Rühl S., Dussurgey S., Dick M.S., Kistner A., Rigard M., Degrandi D., Pfeffer K., Yamamoto M., Henry T, & Broz P. (2015). Guanylate-binding proteins promote AIM2 inflammasome activation during Francisella novicida infection by inducing cytosolic bacteriolysis and DNA release. Nature immunology, 16(5), 476-484.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!