Tregs from young and old mice were cocultured with DC and Teff in 1:4:2 and 1:4:4 (DC/Teff/Treg) ratios in the presence of anti-CD3 antibody for 72 h. In a separate experiment, Treg from young and old mice were cocultured with Teff from young and old mice at 1:1 ratio in the presence of plate-bound anti-CD3 (1 μg mL−1) and soluble anti-CD28 antibody (1 μg mL−1, BD). After 72 h, conditioned media were collected, and IL-10 concentration was measured by using alpha-ELISA (Perkin Elmer, Waltham, MA, USA) as per manufacturer’s instructions.
Alpha elisa
Manufactured by PerkinElmer
Sourced in United States
The Alpha-ELISA is a type of enzyme-linked immunosorbent assay (ELISA) used for detecting and quantifying proteins, peptides, and other analytes in biological samples. It utilizes Alpha technology, a proprietary proximity-based assay platform, to enable sensitive and specific measurements. The core function of the Alpha-ELISA is to provide accurate and reliable quantification of target analytes in a simple and streamlined manner.
Automatically generated - may contain errors
3 protocols using alpha elisa
1
Regulatory T Cell Immunomodulation Assay
2
Treg-Mediated Immune Regulation Across Ages
3
Metabolic Profiling in Diet-Induced Obesity
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Mice from each of the three diet conditions (n = 8/group) were fasted overnight for basal blood glucose measurements. For oral glucose tolerance test (OGTT), mice were fasted overnight followed by oral gavage of dextrose (2 g/kg of body weight). For intraperitoneal insulin tolerance tests (ITT), food was removed from the cage at 9 AM for 4 h fasting prior to intraperitoneal (i.p.) injection of insulin. For both OGTT and ITT, blood glucose was measured at 0, 15, 30, 45, 60, 90, and 120 min. Body mass composition was measured via Echo MRI and subcutaneous, perigonadal, and perirenal fat depots were extracted at sacrifice and weighed. Blood was collected from a new cohort of mice (n = 7–13/group) at the time of sacrifice to measure plasma insulin (Cedarlane Labs, Burlington, Canada), corticosterone (at circadian peak; Enzo Life Sciences, Farmingdale, NY, USA), TNF (Qiagen, Hilden, Germany), IL-1β (Qiagen) and CRP (Life Diagnostics, Inc., WestChester, PA, USA) levels via ELISA. Plasma insulin and leptin from adenovirus treated mice was measured by alpha-ELISA (Perkin–Elmer, MA, USA).
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