All chemicals were of analytical grade, purchased from Sigma-Aldrich (Milano, Italy) and used as received. HPLC-grade acetonitrile and methanol were purchased from Carlo Erba (Milano, Italy); HPLC-grade water was freshly prepared with the Milli-Q purification system (Millipore, Vimodrone, Italy).
Hplc grade acetonitrile
Manufactured by Merck Group
Sourced in Germany, United States, India, Italy, United Kingdom, China, Spain, Sao Tome and Principe, Switzerland, Macao, Canada, France, Indonesia, Poland, Argentina, Japan, Australia
HPLC-grade acetonitrile is a high-purity organic solvent commonly used as a mobile phase component in high-performance liquid chromatography (HPLC) applications. It is a colorless, volatile liquid with a characteristic odor. The product meets the specifications required for HPLC-grade solvents, ensuring consistency and reliability in analytical procedures.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Merck Group (327 mentions)
Formic acid, by Merck Group (195 mentions)
Milli q system, by Merck Group (93 mentions)
Hplc grade methanol, by Merck Group (80 mentions)
Milli q water purification system, by Merck Group (60 mentions)
Acetic acid, by Merck Group (46 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (44 mentions)
Trifluoroacetic acid, by Merck Group (35 mentions)
Ammonium acetate, by Merck Group (34 mentions)
Quercetin, by Merck Group (32 mentions)
Gallic acid, by Merck Group (31 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (31 mentions)
Ethyl acetate, by Merck Group (29 mentions)
Hydrochloric acid (hcl), by Merck Group (29 mentions)
Hplc grade water, by Merck Group (28 mentions)
Ethanol, by Merck Group (28 mentions)
Milli q purification system, by Merck Group (25 mentions)
Sodium hydroxide (naoh), by Merck Group (25 mentions)
Ammonium formate, by Merck Group (24 mentions)
Catechin, by Merck Group (23 mentions)
1 082 protocols using hplc grade acetonitrile
1
Analytical Grade Chemical Analysis
2
Quantitative Analysis of Neurotransmitters
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Standards of dopamine (≥98%), octopamine (≥98%), adrenaline (epinephrine) (≥99%), 9-fluorenyl-methoxycarbonyl chloride (≥99%), dansyl chloride (≥99%) were purchased from Sigma-Aldrich (St. Louis, MO, USA), gabapentin (IS) (≥75%) was obtained from Pfizer (New York, NY, USA). HPLC-grade acetonitrile (“Biosolve”, Jerusalem, Israel), 18.2 MΩ water (Milli-Q, Millipore, Molsheim, France) and formic acid (98%, Acros Organics, Geel, Belgium) were used as the mobile phase. Methanol of HPLC grade was purchased from Vecton (Saint-Petersburg, Russia). Potassium carbonate (≥99%, Vecton, Saint-Petersburg, Russia), potassium bicarbonate (≥99%, Vecton, Saint-Petersburg, Russia), sodium hydroxide (≥99%, Reactive, Saint-Petersburg, Russia), sodium tetraborate (≥99%, Vecton, Saint-Petersburg, Russia), sodium hydrogen phosphate (≥99%, Vecton, Saint-Petersburg, Russia), potassium dihydrogen phosphate (≥99%, Vecton, Saint-Petersburg, Russia), ammonium acetate (≥99%, Vecton, Saint-Petersburg, Russia) and acetic acid were used for the preparation of buffer solutions with pH 10.5, 9.5, 6.5 and 4.5, respectively. Glycine (HPLC grade, Vecton, Saint-Petersburg, Russia), phthalic anhydride (99%, Vecton, Saint-Petersburg, Russia), dimethylformamide (HPLC grade, Vecton, Saint-Petersburg, Russia) were used for PG-Cl synthesis.
3
Preparation of Drug-free Plasma for Analysis
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Drug-free human plasma was obtained from Kuwait Blood Bank, Al Jabriyah, Kuwait. HPLC-grade acetonitrile and other chemicals used in the adopted method were of analytical grade and obtained from Sigma Aldrich, Dorset, UK. “In-house” HPLC grade water was prepared with a MilliQ filter purchased from Millipore, Watford, UK. Syringe membrane filters (13 mm) were purchased from kinesis scientific expert, Cambridgeshire, UK. Nylon solvent filters (0.45 um) were used for solvent filtration and Water 20-positions.
4
Investigating BPA Degradation Pathway
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To investigate the BPA degradation pathway in strain YC-JY1, the BPA metabolites after incubating for 0 h, 3 h, 6 h, 9 h, 12 h, and 24 h were analyzed. The cell cultures (10 mL) were extracted twice using an equal volume of ethyl acetate and then concentrated by rotary evaporator (R-215, Buchi, Switzerland). The residues were dissolved in 500 μL HPLC-grade acetonitrile and filtered using 0.22 μm membrane (Millipore, Bedford, MA, USA) for analysis. HPLC-QTOF-MS/MS (Agilent 6500, Agilent 6545, Palo Alto, CA, USA) was employed to detect the samples.
5
Intracellular Doxorubicin Quantification
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Intracellular DOX levels were analyzed according to an adapted protocol for intracellular DHE measurement [12 (link)]. In short, DOX-challenged iPSC CM were washed twice with PBS + 100 μM diethylenetriaminepentaacetic acid (DTPA; Sigma-Adlrich) and immediately lysed with 100% HPLC-grade acetonitrile (Milipore) using 500 µl per well. Lysates were centrifuged at 10,000×g for 10 min in a cooled table top centrifuge (4 °C, Eppendorf 5418 R). The supernatant was transferred to a fresh tube, dried in a vacuum concentrator (Thermo Fisher Scientific), resuspended in PBS + DTPA and measured with HPLC (Dionex). The injection volume was 25 µl per sample and the flow rate was set to 0.400 ml/min with a pressure of approximately 70 bar. Solution A was > 99% acetonitrile with 0.1% trifluoroacetic acid (TFA; Sigma-Aldrich) and solution B was Milipore grade I-filtered water with 0.1% TFA. Initial solution A to solution B ratio was 85%:15%, gradually shifting to 15%:85% over the measurement period of 40 min. DOX peaks were identified by a reference injection of diluted DOX and appeared after a retention time of approximately 16 min. For each peak, the area under the curve was considered. Peak analysis was carried out with Chromeleon 7 software.
6
Pesticide Stock Solution Preparation
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Aqueous pesticide stock solutions were prepared from analytical grade IMI (≥99.0%) and DIM (≥99.0%), purchased from Dr. Ehrenstorfer (Germany) (Table 1 ). Final pesticide concentrations were prepared by dilution of IMI (51.8 mg/L) and DIM (93.4 mg/L) with calcium chloride solution (0.01 M; POCH, Gliwice, Poland). Basic physicochemical characteristics and chemical structures of pesticides are given in Table 1 . Mobile phase for pesticide analysis consisted of HPLC grade acetonitrile from J.T.Baker (Deventer, Holland) and ultrapure water from a Mili-Q system (Millipore, El Passo, TX, USA). Other chemicals used were listed in our previous publication [47 (link)].
7
Azadirachtin Extraction and Quantification
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Azadirachtin (purity, ≥81%) was purchased from Yunnan Zhongke Biological Industry Co., Ltd. (Kunming, Yunnan, China). The Insect GABA enzyme-linked immunosorbent assay (ELISA) kit, insect glutamate decarboxylase (GAD) ELISA kit, insect CarE ELISA kit, insect GST-s ELISA kit, and insect cytochrome P450 ELISA kit were purchased from Jianglai Biological Co., Ltd. (Shanghai, China). HPLC-grade acetonitrile (J.T. Baker, Phillipsburg, NJ, USA) and ultra-pure water prepared from a Milli-Q purification system (Millipore, MA, USA) were used for semi-preparative HPLC analysis.
Electrospray ionization (ESI) were recorded on Aglient 1290 UPLC/6540, and the 1D and 2D NMR spectra were measured on the Bruker 500 MHz spectrometer, with TMS as the internal standard. RP-18 column (50 μm, YMC Co., Ltd., Kyoto, Japan) gel, MCI gel (75–150 μm, Sci-Bio Chem Co., Ltd., Chengdu, China), and Sephadex LH-20 (40–70 μm, Amersham Pharmacia Biotech AB, Uppsala, Sweden), were used for column chromatography. Semi-preparative HPLC was performed on an YMC Luna C18 (5 μm; 10 × 250 mm) reversed-phase column.
Electrospray ionization (ESI) were recorded on Aglient 1290 UPLC/6540, and the 1D and 2D NMR spectra were measured on the Bruker 500 MHz spectrometer, with TMS as the internal standard. RP-18 column (50 μm, YMC Co., Ltd., Kyoto, Japan) gel, MCI gel (75–150 μm, Sci-Bio Chem Co., Ltd., Chengdu, China), and Sephadex LH-20 (40–70 μm, Amersham Pharmacia Biotech AB, Uppsala, Sweden), were used for column chromatography. Semi-preparative HPLC was performed on an YMC Luna C18 (5 μm; 10 × 250 mm) reversed-phase column.
8
Triterpenoid Compound Characterization
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Natural triterpenoids were purchased from Chengdu Pufei De Biotech Co., Ltd. (Chengdu, Sichuan, China). The purities of all tested compounds were determined by HPLC-UV, which were greater than 98%. Bis-p-nitrophenyl phosphate (BNPP) was purchased from TCI (Tokyo, Japan). Stock solutions of compound 1 –27 were prepared in DMSO and stored at 4°C until use. Phosphate buffer (100 mM, pH 7.4 and pH 6.5) was prepared by using Millipore water and stored at 4°C until use. Human liver microsomes (HLMs) were obtained from Celsis (Shanghai, China). The specific probes DME (hCE1) and DDAB (hCE2) were synthesized in our lab as described previously (Jin et al., 2016 (link); Wang et al., 2016 (link)). Millipore water (Millipore, Bedford, USA), HPLC grade acetonitrile, methanol, and formic acid (Tedia company, USA) were employed throughout the study. The Luciferin Detection Reagent (LDR) was obtained from Promega Corporation (USA). All 1H NMR (400 MHz) and 13C NMR (101 MHz) were recorded on a VARIAN INOVA-400 spectrometer with chemical shifts reported as ppm (in CDCl3, TMS as the internal standard). High resolution MS data were obtained with the LTQ Orbitrap mass spectrometer (Orbitrap Elite).
9
Ginsenoside Profiling and Quantification
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HPLC-grade acetonitrile and methanol were purchased for Fisher Scientific (Waltham, MA); ultrapure water (18.25 MΩ/cm) was prepared by a Mili-Q water system (Millipore, Bedford, USA). Reference ginsenosides, including ginsenosides Rg1, Re, Rf, Rb1, Rg2, Rc, Rh1, Rb2, Rb3, F1, Rd, GXVII, nFe, CO, nFd, F2, G75, Rg3, PPT, Mc, CY, CMx, CK, Rh2, and PPD, were isolated or transformed from ginseng and purified by a series of chromatography procedures in our laboratory. The purity of the twenty-five standards was >95%.
10
Analytical-Grade Chemicals Purification
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All chemicals were of analytical grade, purchased from Sigma-Aldrich (Milano, Italy) and used as received. HPLC-grade acetonitrile and methanol were purchased from Carlo Erba (Milano, Italy); HPLC-grade water was freshly prepared with the Milli-Q purification system (Millipore, Vimodrone, Italy).
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