Uv 2600 pc spectrophotometer

Spectral measurements of the aceclofenac and pluronic samples in different combinations were conducted using Shimadzu UV-2600 PC spectrophotometer with 1 cm quartz cell. Stock solution of Acl 1000 µg ml⁻¹ was prepared by dissolving 0.1 g of pure aceclofenac in 10 ml dimethylsulphoxide (DMSO). Then it was made up to 100 ml by adding distilled water. The solutions of 5% pluronic F108, 3% L81 and mixed pluronic were prepared in 1000 µg ml⁻¹ Acl; and their UV- spectrophotometric measurements were recorded.

UV-vis and fluorescence spectroscopy were utilized to analyze the CdS nanoparticles and the PHV/CdS hybrid films. The UV-vis spectra were obtained using a Shimadzu UV-2600PC spectrophotometer (Shimadzu Corp., Kyoto, Japan), and the measurement range was from 200 nm to 600 nm. The Fluorescence spectra were measured using an F97Pro spectrophotometer (Lengguang Tech., Shanghai, China). The excitation wavelength of the fluorescence measurements was 380 nm, the scanning speed was 3000 nm/min, the scanning interval was 1 nm, the excitation bandwidth was 10 nm, and the emission bandwidth was 10 nm. In order to analyze the morphology of the synthesized CdS particles, transmission electron microscopy (TEM) was used, and carbon foils were used as the supports for the TEM samples. TEM images of the CdS nanoparticles were taken using a Tecnai G2 20 TWIN transmission electron microscope (Hillsboro, OR, USA). The PHV/CdS hybrid films were analyzed using a scanning electron microscope (SEM), and ITO glasses were used as the template. SEM was used to measure the morphology of the CdS and PHV/CdS hybrid films, and images were acquired using a Quanta 200FEG SEM spectrometer at 20 kV, 10 mA, SE mode, and spot size of 3.5 (Hillsboro, OR, USA).