D glucose

Manufactured by Sinopharm

Sourced in China, United States

D-glucose is a monosaccharide, which is the simplest form of carbohydrate. It is the primary source of energy for many organisms and plays a crucial role in various biological processes.

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D (+)‐glucose monohydrate (4 citations) D-glucose anhydrous (2 citations)

32 protocols using d glucose

Podocyte Glucose Sensitivity and HGF Response

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Conditionally immortalized podocytes MPC-5 were kindly provided by Professor Peter Mundel (Albert Einstein College of Medicine, New York, NY, USA) and cultured as previously reported in the literature [15] .
All experiments were performed with differentiated podocytes, free of mycoplasma infection. HG culture medium was made by supplementing normal glucose RPMI 1640 medium (Thermo Gibco, Waltham, MA, USA) containing 5.5 mmol/l D-glucose with additional D-glucose (Sinopharm, Shanghai, China) for a nal D-glucose concentration at 30 mmol/l. Podocytes cultured in normal or high glucose were stimulated with or without 25 ng/ml HGF in the absence or presence of 2.5 μmol/l SU11274 (S1080, Selleck, Houston, TX, USA) for 24 h. SU11274 was added 1 h before HGF supplementation. After treatment, podocytes grown on 60mm culture dishes (Corning, New York, NY, USA) were harvested and the lysate measurement and western blotting were the same as above.

Hou B., Li Y., Li X., Zhang C., Zhao Z., Chen Q., Zhang N, & Li H. (2020). HGF protected against diabetic nephropathy via autophagy-lysosome pathway in podocyte by modulating PI3K/Akt-GSK3β-TFEB axis. Cellular signalling, 75.

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Synthesis and Characterization of Metallic Nanoparticles

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All reagents are of analytical grade and used without further purification. Double distilled water was used throughout the experiments. Cobalt (II) acetate tetrahydrate (Co(OAc)₂·4H₂O), nickel (II) acetate tetrahydrate (Ni(Oac)₂·4H₂O), potassium hexacyanoferrate (II) (K₄[Fe(CN)₆]), potassium ferricyanide (K₃[Fe(CN)₆]), D-(+)-glucose, thioacetamide (TAA), uric acid (UA), and sodium hypophosphite (NaH₂PO₂) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Ascorbic acid (AA), ethanol (EtOH), potassium chloride (KCl), and 4-acetamidophenol (AP) were ordered from Aladdin Industrial Co., Ltd. (Shanghai, China), Yasheng Chemical Co., Ltd. (Jiangsu, China), Lingfeng Chemical Reagent Co., Ltd. (Shanghai, China), and Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China), respectively. Urea and sodium chloride (NaCl) were acquired from Xilong Chemical Co., Ltd. (Guangdong, China), while L-cysteine (Lcy) and D-fructose (Fru) were obtained from Huixing Biochemical Reagent Co., Ltd. (Shanghai, China).

Lang X., Chu D., Wang Y., Ge D, & Chen X. (2022). Defect Surface Engineering of Hollow NiCo2S4 Nanoprisms towards Performance-Enhanced Non-Enzymatic Glucose Oxidation. Biosensors, 12(10), 823.

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Optimized Assay for P. falciparum

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DHA was provided by researcher Yang Lan of China Academy of Traditional Chinese Medical Sciences. CQ, Baf-A1, D-sorbitol and gentamycin sulfate were purchased from BioRuler (Danbury, CT, USA). SYBR Green I nucleic acid gel stain (Invitrogen 10,000 × concentrate in DMSO), labile iron pool probe calcein, AM and SYTO^TM 61 red fluorescent nucleic acid stain were purchased from Invitrogen by Thermo Fisher Scientific (Eugene, OR, USA). Tris-HCL, EDTA, saponin, Triton X-100, hypoxanthine, dimethyl sulfoxide (DMSO) and HEPES were purchased from Sigma Life Science (Spruce Street, St. Louis, MO, USA). RPMI Medium 1640, AlbuMAX II and L-glutamine were purchased from Gibco by Life Technologies (Grand Island, NY, USA). NaHCO₃ and D-Glucose were purchased from Sinopharm Chemical Reagent (Beijing, China). RT-qPCR primers were purchased from Invitrogen Trading (Shanghai, China). RNAsimple Total RNA Kit, FastKing RT Kit (With gDNase) and Talent qPCR PreMix (SYBR Green) were obtained from TIANGEN BIOTECH (Beijing, China). pH probe BCECF, AM was purchased from KeyGEN BioTECH (Nanjing, China). Suspended leukocyte-reduced red blood cells (sickling negative; O rhesus positive) were taken from Red Cross Society of China (Beijing, China).
Plasmodium falciparum laboratory strain 3D7 was maintained long-term preservation by liquid nitrogen and subcultured by our lab.

Tang T., Xu W., Ma J., Wang H., Cui Z., Jiang T, & Li C. (2019). Inhibitory Mechanisms of DHA/CQ on pH and Iron Homeostasis of Erythrocytic Stage Growth of Plasmodium falciparum. Molecules, 24(10), 1941.

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Glucose and Insulin Tolerance Tests

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For the glucose tolerance test (GTT), mice were fasted for 14 h and injected with d-glucose (1.5 g/kg body weight, Sinopharm chemical reagent co., Ltd., 63,005,518) intraperitoneally. For the insulin tolerance test (ITT), mice were fasted for 4 h and injected with recombinant human insulin (0.75 U/kg body weight, Nono Nordisk) intraperitoneally. Blood glucose was monitored with a glucometer (Abbott) at various time points as indicated.

Qiu Y., Sun Y., Xu D., Yang Y., Liu X., Wei Y., Chen Y., Feng Z., Li S., Reyad-ul Ferdous M., Zhao Y., Xu H., Lao Y, & Ding Q. (2018). Screening of FDA-approved drugs identifies sutent as a modulator of UCP1 expression in brown adipose tissue. EBioMedicine, 37, 344-355.

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Synthesis of Graphite-based Nanocomposites

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The graphite was obtained from Sigma-Aldrich (282863). Iron(iii) nitrate nonahydrate, nickel(ii) nitrate hexahydrate, and d-glucose were ordered from Sinopharm Chemical Reagent Co. Ltd, China. Alcohol was purchased from Tianjin Tiantai Fine Chemical Reagent Co. Ltd, China. All chemicals were analytical grade and used directly without further purifying treatments. Deionized water was employed to prepare the solutions throughout.

Li J., Zhang D., Qi H., Wang G., Tang J., Tian G., Liu A., Yue H., Yu Y, & Feng S. (2018). Economical synthesis of composites of FeNi alloy nanoparticles evenly dispersed in two-dimensional reduced graphene oxide as thin and effective electromagnetic wave absorbers. RSC Advances, 8(15), 8393-8401.

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Propofol Mitigates Glucose-Induced Stress in H9c2 Cells

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H9c2 cells were acquired from the Chinese Academy of Science, Shanghai Cell Bank and cultured in low glucose (5.5 mM) minimum essential medium (Gibco-Invitrogen, Grand Island, NY, USA) complemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco BRL, Grand Island, NY, USA), 100 U/mL of penicillin, and 50 g/mL of streptomycin (Beyotime Institute of Biotechnology, China). The cells were placed in a humidified atmosphere containing 5% CO₂ at 37 °C.
To establish a high glucose- (HG-) induced stress model in H9c2 cells, D-glucose (Sinopharm Chemical Reagent Co. Ltd., Shanghai, China) was added in culture medium to reach the final concentration of 22 mM glucose. The concentration of 5.5 mM glucose was used as the control group. A dose-dependent effect of propofol was evaluated by adding 5, 10, 20, and 40 µM of propofol to the cells.

Dong Y., Yan S., Li G.Y., Wang M.N., Leng L, & Li Q. (2020). Identification of key candidate genes and pathways revealing the protective effect of liraglutide on diabetic cardiac muscle by integrated bioinformatics analysis. Annals of Translational Medicine, 8(5), 181.

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Preparation and Characterization of Fe(II/III) EDTA Complexes

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Preparation of Fe(III) EDTA Solution: A 100 mM Fe(III) EDTA solution was prepared with equal molar Na₂EDTA and FeCl₃·6H₂O.
Preparation of Fe(II) EDTA Solution: A 30 mM Fe(II) EDTA solution was prepared with equal molar Na₂EDTA and FeSO₄·7H₂O.
Preparation of Fe(II) EDTA-NO Solution: A 30 mM Fe(II) EDTA solution was equally transferred into two absorption bottles, and N₂ was used as protective gas to pass into NO gas to saturate the solution. The concentration of Fe(II) EDTA-NO was determined before use.
Na₂EDTA·2H₂O (99%), FeCl₃·6H₂O (99%), D-glucose (99.5%) and other chemicals were applied in analytical reagent grade without further purification, which were purchased from Sinopharm Chemical Reagent Co. (Shanghai, China). The compositions of simulated flue gas were supplied by Zhejiang Jingong Gas Co. (Hangzhou, China): N₂ (99.999%), CO₂ (99.999%), O₂ (99.999%), and NO (5 vol% in N₂).

Guo T., Zhang C., Zhao J., Ma C., Li S, & Li W. (2019). Evaluation of polypyrrole-modified bioelectrodes in a chemical absorption-bioelectrochemical reduction integrated system for NO removal. Scientific Reports, 9, 13030.

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Glucose Tolerance Test in Mice

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All mice were fasted on the first night of 24th week. IPGTT was performed the next morning. Mice were injected with a glucose solution (2 g/kg body weight; D-(+)-Glucose, Sinopharm Group Company, Shanghai, China) and blood glucose levels were measured at 0, 15, 30, 60, and 120 min after the glucose load.

Li X., Li M., Xu J., zhang X., Xiao W, & Zhang Z. (2019). Decreased Insulin Secretion but Unchanged Glucose Homeostasis in Cadmium-Exposed Male C57BL/6 Mice. Journal of Toxicology, 2019, 8121834.

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Enzymatic Glucose Biosensor Fabrication

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Glucose oxidase (GOx, EC 1.1.3.4, 100 U/mg, from Aspergillus niger) and bovine serum albumin (BSA) were purchased from MP Biomedicals Co., Ltd. (Shanghai). The multiwalled carbon nanotubes (MWCNTs, >95% purity) were obtained from the Chengdu Organic Chemicals Co., Ltd. of the Chinese Academy of Science (Chengdu, China). Uric acid (UA) was purchased from Sigma. D-glucose, chitosan, glacial acetic acid, glutaraldehyde, vitamin C, L-cysteine (L-cys), and the polytetrafluoroethylene emulsion (60% PTFE) were of analytical grade and were purchased from Sinopharm Chemical Reagent Co., Ltd (SCRC, China).

Tang W., Li L., Wu L., Gong J, & Zeng X. (2014). Glucose Biosensor Based on a Glassy Carbon Electrode Modified with Polythionine and Multiwalled Carbon Nanotubes. PLoS ONE, 9(5), e95030.

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Ammonium Thiomolybdate-Based Catalytic System

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Ammonium thiomolybdate ((NH₄)₂MoS₄), TMB, dopamine hydrochloride (DA, 98%) and N,N,-dimethylformamide (DMF) were purchased from Aladdin Reagent Co., Ltd. l-Lysine (l-Lys) and l-arginine (l-Arg) were purchased from Sangon Biotech Co., Ltd. 30.0% H₂O₂, sodium acetate (NaAc, 99.0%), d(+)-glucose, acetic acid (HAc, 99.5%), hydrazine hydrate (N₂H₄·H₂O), sodium chloride (NaCl, 99.5%), and ammonia chloride (NH₄Cl, 99.5%) were obtained from Sinopharm Chemical Reagent Co., Ltd. Carbon nanotubes were purchased from Chinese Academy of Sciences Chengdu Organic Chemistry Co., Ltd. All reagents were of analytical grade unless otherwise stated.

Zhang X., Wang S., Dao J., Guo J, & Gao Y. (2022). A colorimetric sensing platform for the determination of H2O2 using 2D–1D MoS2-CNT nanozymes. RSC Advances, 12(44), 28349-28358.

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