All experiments were performed with differentiated podocytes, free of mycoplasma infection. HG culture medium was made by supplementing normal glucose RPMI 1640 medium (Thermo Gibco, Waltham, MA, USA) containing 5.5 mmol/l D-glucose with additional D-glucose (Sinopharm, Shanghai, China) for a nal D-glucose concentration at 30 mmol/l. Podocytes cultured in normal or high glucose were stimulated with or without 25 ng/ml HGF in the absence or presence of 2.5 μmol/l SU11274 (S1080, Selleck, Houston, TX, USA) for 24 h. SU11274 was added 1 h before HGF supplementation. After treatment, podocytes grown on 60mm culture dishes (Corning, New York, NY, USA) were harvested and the lysate measurement and western blotting were the same as above.
D glucose
D-glucose is a monosaccharide, which is the simplest form of carbohydrate. It is the primary source of energy for many organisms and plays a crucial role in various biological processes.
Lab products found in correlation
32 protocols using d glucose
Podocyte Glucose Sensitivity and HGF Response
All experiments were performed with differentiated podocytes, free of mycoplasma infection. HG culture medium was made by supplementing normal glucose RPMI 1640 medium (Thermo Gibco, Waltham, MA, USA) containing 5.5 mmol/l D-glucose with additional D-glucose (Sinopharm, Shanghai, China) for a nal D-glucose concentration at 30 mmol/l. Podocytes cultured in normal or high glucose were stimulated with or without 25 ng/ml HGF in the absence or presence of 2.5 μmol/l SU11274 (S1080, Selleck, Houston, TX, USA) for 24 h. SU11274 was added 1 h before HGF supplementation. After treatment, podocytes grown on 60mm culture dishes (Corning, New York, NY, USA) were harvested and the lysate measurement and western blotting were the same as above.
Synthesis and Characterization of Metallic Nanoparticles
Optimized Assay for P. falciparum
Plasmodium falciparum laboratory strain 3D7 was maintained long-term preservation by liquid nitrogen and subcultured by our lab.
Glucose and Insulin Tolerance Tests
Synthesis of Graphite-based Nanocomposites
Propofol Mitigates Glucose-Induced Stress in H9c2 Cells
To establish a high glucose- (HG-) induced stress model in H9c2 cells, D-glucose (Sinopharm Chemical Reagent Co. Ltd., Shanghai, China) was added in culture medium to reach the final concentration of 22 mM glucose. The concentration of 5.5 mM glucose was used as the control group. A dose-dependent effect of propofol was evaluated by adding 5, 10, 20, and 40 µM of propofol to the cells.
Preparation and Characterization of Fe(II/III) EDTA Complexes
Preparation of Fe(II) EDTA Solution: A 30 mM Fe(II) EDTA solution was prepared with equal molar Na2EDTA and FeSO4·7H2O.
Preparation of Fe(II) EDTA-NO Solution: A 30 mM Fe(II) EDTA solution was equally transferred into two absorption bottles, and N2 was used as protective gas to pass into NO gas to saturate the solution. The concentration of Fe(II) EDTA-NO was determined before use.
Na2EDTA·2H2O (99%), FeCl3·6H2O (99%), D-glucose (99.5%) and other chemicals were applied in analytical reagent grade without further purification, which were purchased from Sinopharm Chemical Reagent Co. (Shanghai, China). The compositions of simulated flue gas were supplied by Zhejiang Jingong Gas Co. (Hangzhou, China): N2 (99.999%), CO2 (99.999%), O2 (99.999%), and NO (5 vol% in N2).
Glucose Tolerance Test in Mice
Enzymatic Glucose Biosensor Fabrication
Ammonium Thiomolybdate-Based Catalytic System
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!