Eight days prior to the start of nicotine treatment, animals were randomly assigned to receive either regular chow (CHOW; Harlen Laboratories, Diet 2018, 18% kCal from fat) or HFD (Research Diets, Diet D12451, 45% kcal from fat). After the habituation phase, all animals received new drinking water. Half of the animals in each diet group received a nicotine solution (NIC; 200 μg/mL free base concentration in 2% saccharin vehicle) as has been reported previously (Jung, et al., 2016 (link); King, Caldarone, & Picciotto, 2004 (link)). Control animals received a matched molar solution of L-(+)-tartaric acid (2.5 mM; SigmaAldrich, St. Louis, MO) in 2% saccharin (SAC). NIC and SAC solutions were adjusted to pH 7. (−)-Nicotine hydrogen tartrate salt was provided through the NIDA drug supply program. HFD pellets were changed every 3 days to prevent dehydration, and drinking water solutions were refreshed every 7 days.
Diet 2018
Manufactured by Research Diets
Diet 2018 is a laboratory equipment product offered by Research Diets. It is designed for use in dietary research. The core function of Diet 2018 is to provide a controlled and standardized diet for experimental purposes.
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2 protocols using diet 2018
1
Diet and Nicotine Effects on Animal Models
2
Dietary Effects on Sprague-Dawley Rat Obesity
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8–11-week-old male Sprague–Dawley (SD) rats were purchased from Charles River Laboratories (Wilmington, MA). Rats were cohoused (2 rats/ cage) and maintained on a 12-h light/dark cycle with ad libitum access to chow (Teklad Diet #2018), high fat diet (HF, Research Diets D42151), or high fat diet with oligofructose (HF-OFS, Research Diets D19112708; BENEO, Orafti P95), which is macronutrient and calorie matched to the HF diet with the addition of 10% OFS (Supplementary Table 1 ). Male rats were chosen, as they readily develop obesity when placed on a HF diet and do not have an estrus cycle, which can influence food intake [30 (link)–32 (link)]. Body composition was measured by quantitative magnetic resonance imaging using EchoMRI-1100 (EchoMRI, Houston, Texas), and rats were placed in metabolic cages (Sable Systems International, Las Vegas, NV, USA) for up to 10 days to record food intake and metabolism.
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