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μct 80

Manufactured by Scanco
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Sourced in Switzerland, United States

The μCT 80 is a micro-computed tomography (micro-CT) imaging system designed for high-resolution, non-destructive analysis of small samples. It utilizes X-ray technology to generate 3D images, allowing for detailed visualization and quantification of the internal structure and composition of various materials.

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Lab products found in correlation

μct80 evaluation program v 6.5 1, by Scanco (2 mentions) Solidworks 2016, by Dassault Systèmes (1 mentions) S 4800, by Hitachi (1 mentions) Methacrylic anhydride, by Merck Group (1 mentions) Gelatine type a, by Merck Group (1 mentions) Amira 5, by Visage Imaging (1 mentions) Mimics research 17, by Materialise (1 mentions) Pentobarbital sodium, by Merck Group (1 mentions) Micro ct auxiliary software, by Volume Graphics (1 mentions) Digitaldiagnost, by Philips (1 mentions) Lightspeed vct, by GE Healthcare (1 mentions) Xtremect 1, by Scanco (1 mentions) Image processing language v4.29d, by Scanco (1 mentions) Auxiliary histomorphometric software, by Scanco (1 mentions) Abaqus, by Dassault Systèmes (1 mentions)

93 protocols using μct 80

1

Micro-CT Analysis of Interbody Fusion Device

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Following biomechanical testing, μ-CT scanning was completed on all specimens. Following at least 1 week of fixation in 10% neutral buffered formalin, specimens were trimmed superior to the superior pedicle screw hole and inferior to the inferior screw hole in the axial plane. The resultant tissue section encompassed the vertebral body end plates, the entire disc space, and the interbody device, as well as any resultant callus formation. The specimens were scanned at an isotropic resolution of 37 μ.m (Scanco μCT 80, Scanco USA Inc, Wayne, PA, USA). Quantitative measures of the graft window were performed, including bone volume/total volume (BV/ TV; expressed as a percentage (%)). The mean density of bone volume/mean density of total volume (MDBV/MDTV) was also calculated. As MDBV/MDTV approaches unity, then the region of interest (ROI) is considered to have a more solid architecture and is used to quantify the solidity of bone within the graft window. The ROI was set as a circular disc within the center of the cage, equally spaced from the cage’s mediallateral, anterior-posterior, cranial-caudal boundaries. Morphometric indices were calculated using proprietary software (Scanco μCT 80, Scanco USA Inc).
McGilvray K.C., Easley J., Seim H.B., Regan D., Berven S.H., Hsu W.K., Mroz T.E, & Puttlitz C.M. (2018). Bony ingrowth potential of 3D-printed porous titanium alloy: a direct comparison of interbody cage materials in an in vivo ovine lumbar fusion model. The spine journal : official journal of the North American Spine Society, 18(7), 1250-1260.
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2

Micro-CT Analysis of Interbody Fusion Device

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Following biomechanical testing, μ-CT scanning was completed on all specimens. Following at least 1 week of fixation in 10% neutral buffered formalin, specimens were trimmed superior to the superior pedicle screw hole and inferior to the inferior screw hole in the axial plane. The resultant tissue section encompassed the vertebral body end plates, the entire disc space, and the interbody device, as well as any resultant callus formation. The specimens were scanned at an isotropic resolution of 37 μ.m (Scanco μCT 80, Scanco USA Inc, Wayne, PA, USA). Quantitative measures of the graft window were performed, including bone volume/total volume (BV/ TV; expressed as a percentage (%)). The mean density of bone volume/mean density of total volume (MDBV/MDTV) was also calculated. As MDBV/MDTV approaches unity, then the region of interest (ROI) is considered to have a more solid architecture and is used to quantify the solidity of bone within the graft window. The ROI was set as a circular disc within the center of the cage, equally spaced from the cage’s mediallateral, anterior-posterior, cranial-caudal boundaries. Morphometric indices were calculated using proprietary software (Scanco μCT 80, Scanco USA Inc).
McGilvray K.C., Easley J., Seim H.B., Regan D., Berven S.H., Hsu W.K., Mroz T.E, & Puttlitz C.M. (2018). Bony ingrowth potential of 3D-printed porous titanium alloy: a direct comparison of interbody cage materials in an in vivo ovine lumbar fusion model. The spine journal : official journal of the North American Spine Society, 18(7), 1250-1260.
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3

Customizable PEEK Clips for Spinal Fusion

Cited 1 time
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C-shaped PEEK clips (1 cm diameter × 1 cm depth × 1 cm height) capable of clipping onto standard spinal fusion hardware, specifically the titanium rods used to stabilize the vertebrae, were designed with an internal drug reservoir (0.785 cm3) to house clinically-relevant amounts of deliverable bioactive materials for triggered release, as described previously (Basgul, et al. 2018 , Delaney, et al. 2019 (link)). Briefly, spacer designs were drafted using Solidworks 2016 software (Dassault Systemes, Vélizy-Villacoublay, France) and the Simplify3D slicing program (Simplify3D, Cincinnati, OH), then the PEEK clips were 3D printed by our collaborators at Drexel University using a PEEKMed filament on an Indmatec HPP 155/Gen 2 3D printer (Apium Additive Technologies, Karlsruhe, Germany). The outer-facing surface of the clip was printed with a circular opening 4 mm in diameter (cf., Fig. 1A) for loading the bioactive material into the reservoir. The internal reservoir volume was measured with microCT imaging (Scanco μCT 80, Scanco Medical, Brüttisellen, Switzerland).
Delaney L.J., Basgul C., MacDonald D.W., Fitzgerald K., Hickok N.J., Kurtz S.M, & Forsberg F. (2019). Acoustic Parameters for Optimal Ultrasound-Triggered Release from Novel Spinal Hardware Devices. Ultrasound in medicine & biology, 46(2), 350-358.
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4

Trabecular Microarchitecture Analysis via MicroCT

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The iliac crest biopsy collected from each animal at each time point was used for microCT analysis to quantify the trabecular microarchitecture changes over time. Samples were scanned at a resolution of 10 μm3 at 70 kVp, 113 μA, and 500 ms integration time (Scanco μCT 80, version 1.1.15.0, Scanco USA, Inc., Wayne, PA, USA). One region of interest (ROI) (5 mm diameter, 400 slices) was drawn per sample to include only trabecular bone and reconstructed using fixed optimal threshold values (upper bound = 2760.5 HU, lower bound = 456.7 HU). Threshold bounding was confirmed by visual inspection. The following output measures of trabecular microarchitecture were quantified from the three‐dimensional reconstruction of each ROI cylinder: bone volume fraction (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular spacing (Tb.Sp).
Bisazza K.T., Nelson B.B., Sikes K.J., Nakamura L, & Easley J.T. (2023). Computed Tomography Provides Improved Quantification of Trabecular Lumbar Spine Bone Loss Compared to Dual‐Energy X‐Ray Absorptiometry in Ovariectomized Sheep. JBMR Plus, 7(12), e10807.
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5

Micro-CT Analysis of Bone Structure

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Bones from each animal were scanned via micro-computed tomography (Scanco
μCT 80, Scanco Medical AG, Brüttisellen, Switzerland) with an
isotropic voxel size of 25 µm. Four spatially distributed cylindrical
volumes of interest (VOI) were identified for each tibia and femur based on
anatomical markers21 (link). The
following measurements were obtained: volumetric trabecular material bone
mineral density (mgHA/cm3) (Tb.BMD), trabecular bone volume fraction
(bone volume/total volume, Tb.BV/TV), trabecular number (Tb.N), trabecular
thickness (mm) (Tb.Th), trabecular separation (mm) (Tb.Sp), cortical material
bone mineral density (mgHA/cm3 (link))
(Ct.BMD), cortical bone porosity (Ct.Po), and volume of osteophytes
(mm3)21 (link).
Fischenich K.M., Pauly H.M., Button K.D., Fajardo R.S., DeCamp C.E., Haut R.C, & Haut Donahue T.L. (2016). A Study of Acute and Chronic Tissue Changes in Surgical and Traumatically-Induced Experimental Models of Knee Joint Injury Using Magnetic Resonance Imaging and Micro-Computed Tomography. Osteoarthritis and cartilage, 25(4), 561-569.
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6

Micro-CT Analysis of Femoral Condyles

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An isotropic voxel resolution of 37 μm, 70 kVp tube voltage, 11 μAmp current, and 300 ms integration time was used for μCT (Scanco μCT 80, Scanco Medical AG, Brüttisellen, Switzerland) of the medial and lateral femoral condyles. A 3 mm3 cube ROI was used for analysis of the MFCs, positioned immediately dorsal or ventral to the pin defect, deep to the cortical bone. The pin tract was excluded from the ROI. The contralateral MFC was used to identify a similar ROI for analysis in ESW‐treated MFCs. Outcome parameters for μCT included total volume (TV), bone volume (BV), bone volume fraction (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular spacing (Tb.Sp) using the direct 3D method.21
Stewart H.L., Easley J.T., Selberg K.T., Puttlitz C.M., Nakamura L.K., Johnson J.W, & Kawcak C.E. (2022). Experimental models of bone marrow lesions in ovine femoral condyles. Veterinary Surgery, 52(2), 284-298.
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7

Micro-CT Analysis of Interbody Bone Fusion

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Micro‐computed tomography (μCT) scanning was completed on all specimens following non‐destructive biomechanical testing. Specimens were trimmed superior to the cranial pedicle screw hole and inferior to the caudal screw hole in the transverse plane. The resultant tissue sections encompassed both vertebral body endplates, the intervertebral disc/fusion space, the interbody device, and any callus formation. The specimens were scanned at a resolution of 37 μm (Scanco μCT 80, Scanco USA Inc., Wayne, Pennsylvania) and bone volume fraction (BV/TV), or the volume of bone within the region of interest (ROI) normalized to the total ROI volume, and bone density fraction (MDBV/MDTV), or the mean density of bone within the ROI normalized to the mean density of the total volume of the ROI were calculated. The ROI used for this analysis was the entire volume within the interbody PEEK cage. As BV/TV approaches 100% and MDBV/MDTV approaches unity, then the ROI is considered to have a more solid architecture. μCT analysis was performed in a blinded fashion in which the evaluator was not aware of specimen treatment allocation during scanning or analysis and was unblinded to specimen treatment after data post‐processing.
Gadomski B.C., Labus K.M., Puttlitz C.M., McGilvray K.C., Regan D.P., Nelson B., Seim HB I.I.I, & Easley J.T. (2021). Evaluation of lumbar spinal fusion utilizing recombinant human platelet derived growth factor‐B chain homodimer (rhPDGF‐BB) combined with a bovine collagen/β‐tricalcium phosphate (β‐TCP) matrix in an ovine model. JOR Spine, 4(3), e1166.
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8

Porosity Analysis of FFF Printed Cages

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Micro-CT scanning procedure was conducted on the cages (n=2, per cohort) (Scanco μCT 80 micro-CT scanner,Scanco Medical, Switzerland) [32 ]. To calculate the porosity percentage of the FFF printed cages, two measurements were taken from cage design (n=4 total measurements for each cohort), where a region of interest (ROI) was defined (Fig. 2(a)). The ROI was a solid cuboid having 50 mm3 volume (5x5x2 mm3), which was limited by the cage design (Fig. 2(b)). To calculate the porosity percentage of the cages, segmentation of the dataset was conducted as explained in detailed previously (Scanco Medical, Switzerland) [32 ]. From the outcome of the custom script, the porosity percentage was then calculated as follows:
1−VolumeSolidVolumeTotal
Basgul C., MacDonald D.W., Siskey R, & Kurtz S.M. (2020). Thermal Localization Improves the Interlayer Adhesion and Structural Integrity of 3D printed PEEK Lumbar Spinal Cages. Materialia, 10, 100650.
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9

Ovariectomy's Impact on Maxillary Bone

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At 3 months after ovariectomy, rats were sacrificed under 10% chloral hydrate anaesthesia and maxillae were collected. Both sides of the maxillae were collected from the body and fixed in 4% paraformaldehyde. Samples were scanned using a micro‐CT scanner (Scanco μCT 80, Scanco Medical AG, Bassersdorf, Switzerland) with a 16 μm voxel size. The density of maxilla specimens was standardized to that of hydroxyapatite, and software affiliated to the micro‐CT scanner was used to reconstruct its 3D structure. For alveolar bone, the region of interest (ROI) was chosen in the inter‐radicular region of the right maxillary first molar, keeping away from the roots. The following structural parameters of the ROI were calculated: bone mineral density (BMD), bone volume/tissue volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th) and trabecular separation (Tb.Sp).
Xu H., Zhou S., Qu R., Yang Y., Gong X., Hong Y., Jin A., Huang X., Dai Q, & Jiang L. (2020). Icariin prevents oestrogen deficiency–induced alveolar bone loss through promoting osteogenesis via STAT3. Cell Proliferation, 53(2), e12743.
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10

Microstructural Analysis of Mouse Tibias

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At the end of each experiment, the tibias of the mice were fixed in 4% paraformaldehyde. Samples were scanned using micro-CT (μCT 80; Scanco, Zurich, Switzerland) as previously described (38 (link)). The micro-CT parameters were as follows: voltage, 70 kV; electric current, 114 μA; and resolution, 10 μm per pixel. Three-dimensional structural parameters, including bone volume fraction (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th) and trabecular separation (Tb.Sp), were analyzed (38 (link), 39 (link)).
Du J., He Z., Xu M., Qu X., Cui J., Zhang S., Zhang S., Li H, & Yu Z. (2022). Brown Adipose Tissue Rescues Bone Loss Induced by Cold Exposure. Frontiers in Endocrinology, 12, 778019.
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