Anti perforin apc

Manufactured by BioLegend

Anti-perforin-APC is a monoclonal antibody that binds to the perforin protein, which is a key cytolytic protein involved in the immune response. This antibody is conjugated to the fluorescent dye APC, enabling its detection and quantification in flow cytometry applications.

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Lab products found in correlation

Anti cd3 percp cy5, by BioLegend (2 mentions) Anti cd8 apc cy7, by BioLegend (2 mentions) Anti cd4 pe cy7, by BioLegend (2 mentions) Anti granzyme a pe, by BioLegend (2 mentions) Cytofix cytoperm, by BD (2 mentions) Golgiplug, by BD (2 mentions) Perm buffer, by BD (2 mentions) Anti pd 1 pe cy7, by BioLegend (2 mentions) Anti nkg2d apc, by BioLegend (2 mentions) Anti cd107a pe cy7, by BioLegend (2 mentions) Anti granzyme b fitc, by BioLegend (2 mentions) Anti nkp46 pe cy7, by BioLegend (2 mentions) Anti nkp30 apc, by BioLegend (2 mentions) Anti nkp44 pe, by BioLegend (2 mentions) Anti 2b4 fitc, by BioLegend (2 mentions) Anti cxcr3 apc, by BioLegend (1 mentions) Anti ccr5 pe, by BioLegend (1 mentions) Anti cxcr4 pe, by BioLegend (1 mentions) Anti ccr7 apc, by BioLegend (1 mentions) Anti tnf α apc, by BioLegend (1 mentions)

Close spelling variants of this product

Perforin-APC (13 citations) Anti-Perforin (7 citations) APC anti-human Perforin (3 citations) APC anti-mouse Perforin (2 citations)

6 protocols using anti perforin apc

Flow Cytometry for Immune Cell Profiling

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The following Abs were used for short-term culture or surface marker and ICS for flow cytometry (all Abs were from Biolegend): anti-CD3-PerCP-cy5.5, anti-CD8-APC-Cy7, anti-CD4-PE-Cy7, anti-CCR5-PE, anti-CXCR3-APC, anti-CXCR4-PE, anti-CCR7-APC, anti-granzyme A-PE, anti-Perforin-APC, anti-IFN-γ-PE, and anti-TNF-α-APC. The reagents listed below were all commercial products: brefeldin A (GolgiPlug, BD Biosciences), Cytofix/Cytoperm (BD Biosciences), and Perm buffer (BD Biosciences).

Zou S., Tan Y., Xiang Y., Liu Y., Zhu Q., Wu S., Guo W., Luo M., Shen L, & Liang K. (2022). The Role of CD4+CD8+ T Cells in HIV Infection With Tuberculosis. Frontiers in Public Health, 10, 895179.

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Comprehensive Immunophenotyping of haNK Cells

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The cetuximab (Bristol-Myers Squibb, New York, NY), trastuzumab (Genentech, San Francisco, CA), pertuzumab (Genentech) and isotype (rituximab, Genentech) antibodies were purchased from the National Institutes of Health Pharmacy. Flow cytometry of haNK cells was performed on a BD LSRII flow cytometer (BD Biosciences, San Jose, CA) and analyzed in FlowJo 9.9 (TreeStar Inc., Ashland, OR). Flow cytometry of tumor cells was performed on a BD FACSVerse flow cytometer (BD Biosciences) and analyzed in BD FACSuite (BD Biosciences). Staining of haNK cells was performed with four panels, and the antibodies used were: Anti-CD56-PErCP-Cy5.5, anti-CD16-APC-Cy7, anti-NKG2D-APC, anti-NKG2D-FITC, anti-CD107a-PE-Cy7, anti-GranzymeB-FITC, anti-perforin-APC, anti-NKp46-PE-Cy7, anti-NKp30-APC, anti-NKp44-PE, anti-2B4-FITC, anti-CD158a-PE, anti-CD158d-PE, anti-CD158j-APC, anti-PD-1-PE-Cy7, anti-PD-L1-APC, and anti-MICA-PE; all were obtained from BioLegend (San Diego, CA). Anti-CD56-PE, anti-CD16-PE-Cy7, CD11a-PE, Ki67-FITC, anti-HLA-ABC-FITC and anti-4-1BB-BV421 were obtained from BD Biosciences. In controlled experiments to detect CD16 on haNK cells, the anti-CD16 MAb was CD16-FITC (BD Biosciences) and the isotype control antibody was MIgG1k-FITC (BD Biosciences).

Jochems C., Hodge J.W., Fantini M., Fujii R., Maurice YM I.I., Greiner J.W., Padget M.R., Tritsch S.R., Tsang K.Y., Campbell K.S., Klingemann H., Boissel L., Rabizadeh S., Soon-Shiong P, & Schlom J. (2016). An NK cell line (haNK) expressing high levels of granzyme and engineered to express the high affinity CD16 allele. Oncotarget, 7(52), 86359-86373.

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Multiparameter Flow Cytometry Analysis

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The following antibodies (Abs) were used for short-term culture or surface marker and intracellular cytokine staining for flow cytometry (all Abs were from Biolegend): anti-CD3-PerCP-cy5.5 (Clone UCHT1), anti-CD8-APC-Cy7 (Clone SK1), anti-CD4-PE-Cy7 (Clone RPA-T4), anti-Vδ₁-APC (Clone REA173), anti-Vδ₂-PE (Clone B6), anti-Vγ₂-FITC (Clone 7A5), anti-TNF-α-PE (Clone MAb11), anti-IFN-γ-PE-Cy7 (Clone 4S.B3), anti-interleukin-17A (IL-17A)-PE-Cy7 (Clone BL168), anti-Granzyme A-PE (Clone CB9), anti-Perforin-APC (Clone dG9), anti-granulocyte macrophage colony-stimulating factor (GM-CSF)-APC (Clone BVD2-21C11). The isotype control mAbs were purchased from the related company, respectively. The reagents listed below were all commercial products: brefeldin A (GolgiPlug, BD Biosciences), Cytofix/Cytoperm, and Perm buffer (BD Biosciences). The isotype control mAbs were purchased from the related company, respectively.

Zou S., Xiang Y., Guo W., Zhu Q., Wu S., Tan Y., Yan Y., Shen L., Feng Y, & Liang K. (2022). Phenotype and function of peripheral blood γδ T cells in HIV infection with tuberculosis. Frontiers in Cellular and Infection Microbiology, 12, 1071880.

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Multiparameter Flow Cytometry of NK Cells

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Flow cytometry of NK cells was performed on a BD LSRII flow cytometer (BD Biosciences, San Jose, CA) and analyzed in FlowJo 9.9 (TreeStar Inc., Ashland, OR). Staining of NK cells was performed with four panels, and the antibodies used were: anti-CD56-BV605, anti-NKG2D-APC, anti-CD107a-PE-Cy7, anti-GranzymeB-FITC, anti-perforin-APC, anti-NKp46-PE-Cy7, anti-NKp30-APC, anti-NKp44-PE, anti-2B4-FITC, anti-4-1BB-PerCP-Cy5.5, anti-CD95-BV421, anti-Tim3-BV421, anti-TRAIL-PE, anti-CD122-PerCP-Cy5.5, anti-CD122-BV510, anti-CD95L-PE, anti-Ki67-BV421, anti-CD40L-BV510, anti-PD-1-PE-Cy7; all were obtained from BioLegend (San Diego, CA). Anti-CD16-APC-H7, PD-L1-PE-Cy7, and CD11a-FITC, were obtained from BD Biosciences (San Jose, CA). Anti-NKG2A-PE was from R&D Systems (Minneapolis, MN), and anti-CD158a-PerCP-Cy5.5 from eBioscience.

Jochems C., Tritsch S.R., Pellom S.T., Su Z., Soon-Shiong P., Wong H.C., Gulley J.L, & Schlom J. (2017). Analyses of functions of an anti-PD-L1/TGFβR2 bispecific fusion protein (M7824). Oncotarget, 8(43), 75217-75231.

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Assessing Immune Cell Activation in Tumor Samples

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Mononuclear cells isolated from fresh tumor specimens were stimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) + 1 μg/ml ionomycin in the presence of anti-CD107a FITC monoclonal antibody (BioLegend) for 1 h followed by 3 h incubation with brefeldin A (BioLegend). Unstimulated cells were used as control. Cells were then washed in PBS, stained with anti-CD45 PerCP (EXBIO), anti-CD3 Alexa Fluor 700 (EXBIO) or APC-eFluor780 (eBioscience), anti-CD4 ECD (Beckman Coulter), anti-CD8 HV500 (BD Biosciences) and anti-CD56 Alexa Fluor 700 (BioLegend) monoclonal antibodies, fixed in fixation/permeabilization buffer (eBioscience), permeabilized with permeabilization buffer (eBioscience) and stained with anti-IFN-γ PE-Cy7 (eBioscience), anti-granzyme B Brilliant Violet 421 (BD Biosciences) and anti-perforin APC (BioLegend) monoclonal antibodies. The percentage of CD3⁺CD8⁺ T cells and CD3⁻CD56⁺ NK cells producing IFN-γ and degranulating upon PMA/ionomycin stimulation were determined by flow cytometry. The data were analyzed with the FlowJo software package (Tree Star, Inc.).

Truxova I., Kasikova L., Hensler M., Skapa P., Laco J., Pecen L., Belicova L., Praznovec I., Halaska M.J., Brtnicky T., Salkova E., Rob L., Kodet R., Goc J., Sautes-Fridman C., Fridman W.H., Ryska A., Galluzzi L., Spisek R, & Fucikova J. (2018). Mature dendritic cells correlate with favorable immune infiltrate and improved prognosis in ovarian carcinoma patients. Journal for Immunotherapy of Cancer, 6, 139.

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Multicolor Flow Cytometry Analysis of Liver and Spleen Lymphocytes

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Lymphocytes from the liver and spleen of mice were isolated as previously described²³ (link) and analyzed by multicolor flow cytometry. Flow cytometry was performed on a CytoFLEX LX platform and results were analyzed using FlowJo software. The following antibodies were used in this study (clone, manufacture): anti-F4/80-Alexa Fluor 700 (BM8, BioLegend), anti-B220-Alexa Fluor 700 (RA3-6B2, BioLegend), anti-Cd11b-Alexa Fluor 700 (M1/70, BioLegend), anti-Cd3-Alexa Fluor 594 (17A2, BioLegend) anti-Cd4-BV605 (GK1.5, BioLegend), anti-Cd8-BV786 (53-6.7, BD), anti-Foxp3-BV421 (MF-14, BioLegend), anti-Cd62l-PerCP/Cy5.5 (MEL-14, BioLegend), anti-Cd44-BV510 (IM7, BioLegend), anti-NK1.1-BV510 (PK136, BioLegend), anti-A2Ar-FITC (7F6-G5-A2, NOVUS), anti-Cd73-APC (TY11.8, BioLegend), anti-Cd39-PECy7 (Duha59, BioLegend), anti-Cd19-PerCP Cy5.5(6D5, BioLegend), anti-PD1-APC/Cy7 (29F.1A12, BioLegend), anti-Cd107a- PE (1D4B, BD), anti-CD69-BV650 (H1.2F3, BioLegend), anti-Ifnγ-BV421 (XMG1.2, BioLegend), anti-Tnfα-PerCPCy5.5 (MP6-XT22, BioLegend), anti-GZMB-FITC (GB11, BioLegend), and anti-perforin-APC (S16009A, BioLegend).

Myojin Y., McCallen J.D., Ma C., Bauer K.C., Ruf B., Benmebarek M.R., Green B.L., Wabitsch S., McVey J.C., Fu C., Xie C, & Greten T.F. (2023). Adenosine A2a receptor inhibition increases the anti-tumor efficacy of anti-PD1 treatment in murine hepatobiliary cancers. JHEP Reports, 6(1), 100959.

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