Az100 stereomicroscope

Manufactured by Nikon

Sourced in Japan

The AZ100 stereomicroscope is a versatile optical instrument designed for a range of laboratory and research applications. It provides high-quality magnification and clear, detailed imaging of specimens. The AZ100 features binocular eyepieces, a built-in LED illumination system, and a stable, ergonomic design to support precise observation and analysis.

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Lab products found in correlation

Mp e 65 mm lens, by Canon (1 mentions) Eos kiss x9, by Nikon (1 mentions) Eclipse e600 microscope, by Nikon (1 mentions) Focus pro 7, by Helicon (1 mentions) Fused silica capillary, by Molex (1 mentions) Prime bsi express camera, by Teledyne (1 mentions) Bm purple solution, by Roche (1 mentions) Digoxigenin utp, by Roche (1 mentions) Matrigel matrix, by BD (1 mentions)

5 protocols using az100 stereomicroscope

New Metapone ant species described

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Types.Metaponequadridentata Eguchi, 1998. Three paratypes from East Malaysia (Borneo), Sabah, Poring, Kinabalu were examined. Two paratype workers (SKYC) and one paratype queen (MNHAH).
Source images of the new species for focus stacking were taking using a Canon EOS Kiss X9 digital camera, attached to a Nikon AZ100 stereomicroscope (for worker, queen, and male bodies, excluding male genitalia), and a Nikon Eclipse E600 microscope (for male genitalia). Focus-stacked images were produced using Helicon Focus Pro 7.0.2 (Helicon Soft Ltd., http://www.heliconsoft.com/), and improved with the retouching function of the same software. Colour balance and contrast were adjusted using GIMP 2.8 (The GIMP Development Team, http://www.gimp.org). Two paratype workers of M.quadridentata were imaged on a Dun Inc™ Passport II macrophotography imaging system, using a Canon MP-E 65 mm lens. Focus-stacked images of the two paratypes were produced using Zerene Stacker (Zerene Systems LLC, http://zerenesystems.com/cms/stacker). The final images were further adjusted, annotated, and scale bars added using Adobe Photoshop CS6. All specimens of the new species examined are deposited in the Lee Kong Chian Natural History Museum, under the Zoological Reference Collection (ZRC).
High resolution image plates are available at https://doi.org/10.5061/dryad.9776725

Wang W.Y., Yamada A, & Eguchi K. (2019). Discovery of a new ant species of the elusive termitophilous genus Metapone in Singapore (Hymenoptera, Formicidae, Myrmicinae), with the first detailed description of male genitalia of the genus. ZooKeys, 876, 125-141.

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Imaging EGFP-Positive Lymphocytes in Zebrafish

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Live adults were imaged using epifluorescence after transient anesthesia. EGFP positive lymphocytes were imaged in the caudal fin using a Nikon AZ100 Stereomicroscope at 20 frames per second [18 (link)].

Rothschild S.C., Lai G., Tombes R.M, & Clements W.K. (2023). Constitutively active CaMKII Drives B lineage acute lymphoblastic leukemia/lymphoma in tp53 mutant zebrafish. PLOS Genetics, 19(12), e1011102.

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Laser-Induced Fluorescence Detection in μFFE

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For laser-induced fluorescence (LIF)
detection, a 50 mW beam from a variable power 488 nm 150 mW diode
pumped solid state laser (Sapphire, Coherent, Santa Clara, CA) was
expanded to a ∼2.5 cm wide by ∼150 μm thick line
across the μFFE separation channel. To investigate broadening
effects, replicate separations were performed with the detection line
positioned several distances from the top of the separation channel.
For on-capillary detection, a window was burned in a 360 μm
O.D., 20 μm I.D. fused silica capillary (Polymicro Technologies,
Phoenix, AZ) and positioned under the laser line.
Fluorescence
imaging across the separation channel was performed using a Prime
BSI Express camera (Teledyne Photometrics, Tucson, AZ) mounted on
an AZ 100 stereomicroscope (Nikon, Tokyo, Japan) with a 0.5×
objective. Fluorescent dye separations were imaged using an exposure
time of 200 ms and an Endow GFP filter cube (Nikon) containing a dichroic
mirror (495 nm cutoff) and a 500–550 nm band-pass filter. Separations
of Chromeo P503-labeled proteins were imaged using an exposure time
of 500 ms and a custom filter cube (Nikon) containing a dichroic mirror
(500 nm cutoff) and a 570–640 nm bandpass filter.

Douma C.C, & Bowser M.T. (2023). Assessing Surface Adsorption in Cyclic Olefin Copolymer Microfluidic Devices Using Two-Dimensional Nano Liquid Chromatography-Micro Free Flow Electrophoresis Separations. Analytical Chemistry, 95(50), 18379-18387.

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Genetic Manipulation of Chick Gastrointestinal Development

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Fertilized White Leghorn eggs from Haas Farm (France) were incubated at 38°C in humidified incubators. Gastrointestinal tissues from chick embryos were dissected as described (18 (link)). Retroviral constructs were transfected in DF-1 cells to produce retroviruses. Retroviruses were injected into the splanchnopleural mesoderm of Stage-10 chicken embryos to target the stomach mesenchyme (9 (link),18 (link)). Eggs were then placed at 38°C until harvested. In situ hybridization experiments on stomach were carried out as described (10 (link)). Antisense NOGGIN riboprobes were generated using the chick NOGGIN template (9 (link)) by reverse transcription with incorporation of digoxigenin-UTP (Roche). Anti-digoxigenin antibodies coupled to alkaline phosphatase (Roche) were used to detect NOGGIN mRNA/antisense probe complexes with the BM Purple solution (Roche). Images were acquired using a Nikon-AZ100 stereomicroscope.

Sagnol S., Yang Y., Bessin Y., Allemand F., Hapkova I., Notarnicola C., Guichou J.F., Faure S., Labesse G, & de Santa Barbara P. (2014). Homodimerization of RBPMS2 through a new RRM-interaction motif is necessary to control smooth muscle plasticity. Nucleic Acids Research, 42(15), 10173-10184.

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Chick Embryo Xenograft for GIST-T1 Cells

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Fertilized White Leghorn eggs from Les Bruyeres Farm (France) were incubated at 38°C in humidified incubators. After 2 days of incubation, 3 mL of albumin was removed with a sharp needle, and an approximately 10 mm opening was created at the top by carefully minimizing any contamination of the interior with shell fragments. The opening was sealed with scotch‐tape and eggs incubated for 5 days. At day 7 of embryonic development (E7), the opening was enlarged to about 20 mm to allow grafting. For each condition, 1 × 10⁶ GIST‐T1 cells were resuspended in 25 μL serum‐free medium and 25 μL Matrigel Matrix (BD Biosciences). After polymerization into a drop at 37°C for 5 minutes, cells were implanted at the top of the CAM. At day 5 post‐graft, the GIST cell grafts were excised with the surrounding CAM, fixed in 4% paraformaldehyde, embedded in paraffin, cut in 10 μm sections, and processed for histological (haematoxylin‐eosin solution) or immunohistochemistry analysis, as previously described.⁶, ²⁷ Images were acquired using a Nikon‐AZ100 stereomicroscope.

Guérin A., Martire D., Trenquier E., Lesluyes T., Sagnol S., Pratlong M., Lefebvre E., Chibon F., de Santa Barbara P, & Faure S. (2020). LIX1 regulates YAP activity and controls gastrointestinal cancer cell plasticity. Journal of Cellular and Molecular Medicine, 24(16), 9244-9254.

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