Glycerol

Manufactured by Maclin Biochemical Technology

Sourced in China

Glycerol is a clear, odorless, and viscous liquid used in various laboratory applications. It is a simple polyol compound with the chemical formula C3H8O3. Glycerol has a wide range of applications in the scientific field due to its unique physical and chemical properties.

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Glycerol (gly, (2 citations)

29 protocols using glycerol

Cytotoxicity and Apoptosis Assay of C3G

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Cyanidin-3-O-glucoside (C3G) was offered from Chengdu Gelip Biotechnology Co.; Ltd. (Chendu, Sichuan Province, China). Choline chloride, glycerol, 1,3-butanediol, 1,4-butanediol, glycol, acetonitrile, and sodium acetate buffer of analytical purity were purchased from Shanghai McLean Biochemical Technology Co.; Ltd. (Shanghai, China). AB-8 macroporous resin and Sephadex LH-20 were offered from Tianjin Tianxin Fine Chemical Factory (Tianjin, China). Human hepatoma cells (HepG2 cells) and Human normal hepatic cell (HL-7702 cells) were afforded from Concorde Cell Repository (Beijing, China). The fetal bovine serum (FBS) was from GIBCO-BRL (Grand Island, NY, USA). MTT was obtained from Beijing Baileibo Technology Co.; Ltd. (Beijing, China). RMPI-1640 medium, penicillin, and streptomycin were provided from Beijing Shengmu Biotechnology Co.; Ltd. (Beijing, China). Trypsin was obtained from Beijing Fubo Biotechnology Co.; Ltd. (Beijing, China). Annexin V-FITC/PI cell apoptosis detection and ROS kits were from Shanghai Yisheng Biotechnology Co.; Ltd. (Shanghai, China). Formic acid, ethanol, and potassium chloride buffer were purchased from Shanghai Jinjinle Industrial Co.; Ltd. (Shanghai, China).

Xue H., Tan J., Li Q., Tang J, & Cai X. (2020). Ultrasound-Assisted Deep Eutectic Solvent Extraction of Anthocyanins from Blueberry Wine Residues: Optimization, Identification, and HepG2 Antitumor Activity. Molecules, 25(22), 5456.

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Cyanidin-3-O-glucoside Extraction Protocol

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Cyanidin-3-O-glucoside (C3G) was purchased from Chengdu Gelip Biotechnology Co., Ltd. (Chendu, China). Choline chloride, glycerol, 1,3-butanediol, 1,4-butanediol, glycol, EtOH and sodium acetate buffer of analytical purity were afforded from Shanghai McLean Biochemical Technology Co., Ltd. (Shanghai, China). AB-8 macroporous resin was offered from Tianjin Bohong Resin Technology Co., Ltd. (Tianjin, China). Formic acid, potassium chloride buffer and hydrochloric acid were from Shanghai Jinjinle Industrial Co., Ltd. (Shanghai, China). BuOH, acetonitrile (ACN), trifluoroacetic acid (TFA) and methyl tert butyl ether (MTBE) were obtained from Nanjing Huijing Petrochemical Co., Ltd. (Nanjing, China).

Xue H., Tan J., Li Q., Tang J, & Cai X. (2020). Optimization Ultrasound-Assisted Deep Eutectic Solvent Extraction of Anthocyanins from Raspberry Using Response Surface Methodology Coupled with Genetic Algorithm. Foods, 9(10), 1409.

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Peptide-Graphene Oxide Bioconjugate

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All chemicals used in this work are of analytical level. Peptide with the sequence of KIIIIKYWYAF was bought from the SynPeptide Biotechnology Co., Ltd. (Nanjing, China). Monolayered GO aqueous dispersions (GO-1, 10 mg/g) were provided by the Hangzhou Gaoxi Technology Co., Ltd. (Hangzhou, China). D-anhydrous glucose (AR), phosphoric acid, ethanol, glycerol, glutaraldehyde, NaCl, tryptone, and agar powder were purchased from the Shanghai Maclean Biochemical Technology Co., Ltd. (Shanghai, China).

He P., Yang M., Lei Y., Guo L., Wang Y, & Wei G. (2023). Solvent-Evaporation-Induced Synthesis of Graphene Oxide/Peptide Nanofiber (GO/PNF) Hybrid Membranes Doped with Silver Nanoparticles for Antibacterial Application. Polymers, 15(5), 1321.

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Synthesis and Characterization of Hydrogel Biomaterials

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Hyaluronic acid (HA) was purchased from Shandong Focusfreda Biotech Co., Ltd, China. Chitosan (CS), adipic acid dihydrazide, potassium chloride (KCl), glycerol, sodium periodate, ethylene glycol, and N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) were purchased from Shanghai Macklin Biochemical Co., Ltd, China. 1-Hydroxybenzotriazole anhydrous (HOBT, 98%) was purchased from J&K. 2-(4-morpholino) ethanesulfonic acid (MES) was brought from Sigma-Aldrich Life Science. All reagents were used as received. Deionized water (DI water) was used for all experiments.

Hu Y., Liu N., Chen K., Liu M., Wang F., Liu P., Zhang Y., Zhang T, & Xiao X. (2022). Resilient and Self-Healing Hyaluronic Acid/Chitosan Hydrogel With Ion Conductivity, Low Water Loss, and Freeze-Tolerance for Flexible and Wearable Strain Sensor. Frontiers in Bioengineering and Biotechnology, 10, 837750.

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Synthesis of metal nitrate precursors

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Glycerol (ACS, ≥99.5%) were purchased from Macklin Biochemical Co., Ltd (Shanghai, China). Iron nitrate nonahydrate, cobalt nitrate hexahydrate, nickel nitrate hexahydrate, cupric nitrate trihydrate, zinc nitrate hexahydrate and 50% manganous nitrate solution, dimethyl carbonate (DMC), diethyl carbonate (DEC), Glycerol carbonate (GC), n-butanol, benzyl alcohol were all analytical reagent grade and obtained from Sinopharm Chemical Reagent Co., Ltd. Others were also of analytical grade and used as-received without further purification.

Xiang M, & Wu D. (2018). Transition metal-promoted hierarchical ETS-10 solid base for glycerol transesterification. RSC Advances, 8(58), 33473-33486.

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Synthesis and Characterization of 1-Allyl-3-Methylimidazolium Chloride

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The bleached hardwood-dissolving pulp (DP~548, α-cellulose~88.74%, element content: C~41.49 %, H~6.08%, O~50.78%) was obtained from a pulp mill in Jining, China. N-methylimidazole (Analytical Reagent (AR), >98%) and 3-allyl chloride (AR, >99%) were purchased from Macklin Inc Company (Shanghai, China), and were distilled before use. Urea (AR, >99%), glycerol (AR, >99.5%) and choline chloride (AR, >98%) were purchased from Macklin Inc., Company (Shanghai, China) and were used without further purification.
The 1-allyl-3-methylimidazolium chloride (AMIMCl) was synthesized according to a method reported in the literature [14 (link)]. The purity of ~98% was determined by nuclear magnetic resonance spectroscopy (AVANCEII400, Bruke Inc., Billerica, Germany) ((400 MHz, DMSO-d6), 9.882 (s, 1H, NCHN), 8.06~8.08 (d, 2H, NCHCHN), 6.03 (ddt, 1H, CH₂CH₁), 5.23 (m, 2H, CH₂CH), 5.005 (d, 2H, CH₂N), 3.934 (s, 3H, NCH₃)) and a water content of ~0.51% was verified by a Karl Fischer moisture meter (AKF-1, Mettler Toledo International Co., LTD, Zurich, Switzerland).

Xue Y., Li W., Yang G., Lin Z., Qi L., Zhu P., Yu J, & Chen J. (2022). Strength Enhancement of Regenerated Cellulose Fibers by Adjustment of Hydrogen Bond Distribution in Ionic Liquid. Polymers, 14(10), 2030.

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Fabrication of Polymer-Based Electrochemical Devices

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PVA (M_w: 61,000) was purchased from Sigma Aldrich Co., Ltd. Glycerol (ACS, 99.5%) and calcium chloride (CaCl₂) anhydrous was offered by Shanghai Macklin Biochemical Co., Ltd. Sodium alginate (AlgNa) (200–500 mPa.s) was bought from Shenzhen Dieckmann Tech Co., Ltd. Poly(3,4 ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS, 5.0 wt. %, conductive screen printable ink) was supplied by Sigma Aldrich Co., Ltd. The PI membrane was kindly provided by Changchun Gao Qi polyimide material Co., Ltd, China. All reagents were used without further treatment.

Yang S., Zhang L., Mao J., Guo J., Chai Y., Hao J., Chen W, & Tao X. (2024). Green moisture-electric generator based on supramolecular hydrogel with tens of milliamp electricity toward practical applications. Nature Communications, 15, 3329.

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Synthesis and Characterization of Stimuli-Responsive Nanoparticles

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A gallium (Ga) and indium (In) were purchased from local company (China). N-Isopropylacrylamide (NIPAm) and glycerol were obtained from Shanghai Macklin Biochemical Co. Ltd. (Shanghai, China). N,N′-Methylenebis(acrylamide) (MBA) was purchased from Sigma-Aldrich (WUXI) Life Science & Technology Co. Ltd. (Wuxi, China). Ammonium persulfate (APS) was obtained from Aladdin Industrial Corporation (Shanghai, China). Doxorubicin hydrochloride (DOX·HCl) was purchased from Shanghai D&B Biological Science and Technology Co. Ltd. (Shanghai, China). All the chemicals were pure and used directly without further purifying. Dulbecco's Modification of Eagle's Medium (DMEM) was purchased from Wisent (China). Fetal bovine serum (FBS), penicillin/streptomycin and phosphate buffered saline (PBS, pH 7.4) were obtained from Thermo Fisher Scientific Corporation (China). Cell counting kit-8 (CCK-8) was purchased from Dojindo Laboratories (China). Acridine orange (AO) and ethidium bromide (EB) solution were obtained from Solarbio (China). 4T1 mouse breast cancer cells were supplied by American Type Culture Collection (ATCC).

Fan L., Sun X., Wang X., Wang H, & Liu J. (2019). NIR laser-responsive liquid metal-loaded polymeric hydrogels for controlled release of doxorubicin. RSC Advances, 9(23), 13026-13032.

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Enzyme-catalyzed Glucopyranoside Assay

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4-Nitrophenyl-α-D-glucopyranoside (p-NPG) and α-glucosidase were provided by Sigma-Aldrich Chemical Co., Ltd. (Shanghai, China). Organic solvents and the Folin–Ciocalteu reagent were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Arecoline hydrobromide, rutin trihydrate, and gallic acid were purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Choline chloride (98%), betaine (98%), L-proline (99%), glucose (99%), lactic acid (88%), xylitol (99%), malic acid (98%), urea (99%), citric acid (99.5%), glycerol (99%), 1,4-butanediol (98%), ethylene glycol (99.5%), and AB-8 macroporous adsorption resin were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). All these reagents were of analytical grade.

Liu J., Ma L., Deng S., Chen X., Li Q., Xu A., Tong T., Tan S., Wang M., Cai J, & Wang H. (2024). Deep Eutectic Solvents for Efficient and Selective Extraction of α-Glucosidase Inhibitors from Waste Seeds of Refined Betel Nuts. Foods, 13(7), 1070.

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Extraction and Characterization of Dried LSP

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The dried LSP discarded during processing were collected from Hangzhou, Zhejiang province, China, and dried with hot air at 45 °C until reaching a constant weight. Folin-Ciocalteu phenol reagent, quercetin, vanillin, and glycerol were purchased from Macklin, gallic acid, DPPH, ABTS, 2,4,6-tripyridyl-s-triazine (TPTZ), ferric chloride, sodium carbonate (Na₂CO₃), sodium nitrite (NaNO₂₎, aluminum chloride (AlCl₃), sodium hydroxide (NaOH), methanol, hydrochloric acid, catechin, Folin-Denis reagent, tannin acid, Trolox, phosphate, potassium ferricyanide, trichloroacetic acid, ferric chloride, and L-ascorbic acid were purchased from Aladdin Industrial Co. (Shanghai, China). All solvents of HPLC grade were purchased from Aladdin Industrial Co., Ltd. (Shanghai, China).

Bao N., Wang D., Fu X., Xie H., Gao G, & Luo Z. (2021). Green Extraction of Phenolic Compounds from Lotus Seedpod (Receptaculum Nelumbinis) Assisted by Ultrasound Coupled with Glycerol. Foods, 10(2), 239.

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