Hcclm3

Huh7, SMMC7721, QGY7703, L-02, BEL7404, GSG7701, HCCLM3, MHHC97H, H22, and HCT116 were purchased from the China Infrastructure of Cell Line Resources (Beijing, China). HepG2, Hep3B, U-2 OS, SK-HEP-1, PLC/PRF/5, and H1299 were purchased from the American Type Culture Collection (Manassas, VA, USA). U-2 OS, Huh7, HepG2, Hep3B, SK-HEP-1, PLC/PRF/5, HCT116, and H1299 were cultured in DMEM supplemented with 10% (v/v) fetal bovine serum (FBS) (Invitrogen, CA, USA). SMMC7721, L-02, BEL7404, QGY7703, GSG7701, HCCLM3, MHHC97H, and H22 were cultured in 1640 supplemented with 10% (v/v) FBS (Invitrogen, CA, USA). All cells were maintained at 37°C and 5% CO₂.

Hepa1-6, Renca and A20 cells were purchased from the American Type Culture Collection (ATCC). L-02, Huh7, HCCLM3, MHHC97H, Cal-27, FaDu and Detroit 562 were purchased from the China Infrastructure of Cell Line Resources (Beijing, China). CEN1 and CEN2 were kindly provided by H.L. Chen (The University of Hong Kong). The other human cell lines were purchased from the ATCC. Tumor cell lines and normal cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 µg/ml streptomycin. Primary human umbilical vein endothelial cells (HUVECs) were purchased from ScienCell and cultured according to the manufacturer’s instructions. Primary mouse hepatocytes were isolated by the two-step collagenase perfusion technique and cultured in plates pre-coated with 0.1% rat tail collagen (SIGMA-ALDRICH).²⁴ Hybridomas were cultured in RPMI 1640 containing 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 µg/ml streptomycin. All cell lines and assay cultures were maintained at 37°C and 5% CO₂.