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Au2700 autoanalyser

Manufactured by Olympus
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Sourced in Japan, Germany

The AU2700 Autoanalyzer is a compact, automated clinical chemistry analyzer designed for routine in-vitro diagnostic testing. It utilizes spectrophotometric analysis to quantitatively determine the levels of various analytes in biological samples such as blood, urine, and other bodily fluids.

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Lab products found in correlation

Ventana machine, by Roche (1 mentions) Freestyle optium neo, by Abbott (1 mentions)

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AU2700

7 protocols using au2700 autoanalyser

1

Liver Histopathology and Enzyme Analysis

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Mouse liver fragments were fixed in 4% paraformaldehyde and embedded in paraffin for immunohistochemistry. For histopathology, hematoxylin and eosin (H&E) staining of liver tissues was carried out to investigate the liver injury. Plasma alanine (ALT) and aspartate (AST) transaminases were measured according to the IFCC primary reference procedures using Olympus AU2700 Auto- analyser® (Olympus Optical, Tokyo, Japan).
Farooq M., Filliol A., Simoes Eugénio M., Piquet-Pellorce C., Dion S., Raguenes-Nicol C., Jan A., Dimanche-Boitrel M.T., Le Seyec J, & Samson M. (2019). Depletion of RIPK1 in hepatocytes exacerbates liver damage in fulminant viral hepatitis. Cell Death & Disease, 10(1), 12.
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2

Liver Injury Histopathology and Enzymes

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Fragments of mouse livers were fixed in 4% paraformaldehyde and embedded in paraffin for IHC and hematoxylin and eosin (H&E) staining. For histopathology, H&E staining of liver tissues was carried out to investigate liver injury. Serum aspartate and alanine transaminases (ALT and AST, respectively) were measured according to the IFCC primary reference procedures using Olympus AU2700 Autoanalyser® (Olympus Optical, Tokyo, Japan).
Filliol A., Farooq M., Piquet-Pellorce C., Genet V., Dimanche-Boitrel M.T., Vandenabeele P., Bertrand M.J., Samson M, & Le Seyec J. (2017). RIPK1 protects hepatocytes from death in Fas-induced hepatitis. Scientific Reports, 7, 9205.
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3

Histological Liver Injury Assessment

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Fragments of mouse livers were fixed in 4% paraformaldehyde and embedded in paraffin for IHC and hematoxylin and eosin (H&E), or frozen in isopentane cooled with liquid nitrogen for immunofluorescence studies. For histopathology, H&E staining of liver tissues was carried out to investigate liver injury. Serum ALT and AST transaminases was measured according to the IFCC primary reference procedures using Olympus AU2700 Autoanalyser® (Olympus Optical, Tokyo, Japan).
Filliol A., Piquet-Pellorce C., Le Seyec J., Farooq M., Genet V., Lucas-Clerc C., Bertin J., Gough P.J., Dimanche-Boitrel M.T., Vandenabeele P., Bertrand M.J, & Samson M. (2016). RIPK1 protects from TNF-α-mediated liver damage during hepatitis. Cell Death & Disease, 7(11), e2462-.
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4

Histopathological Analysis of Liver

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The histopathological analysis of liver was performed using haematoxylin & eosin–safran staining. Levels of serum alanine and aspartate transaminases (ALT and AST) were assessed according to the IFCC primary reference procedures using Olympus AU2700 Autoanalyser® (Olympus Optical, Tokyo, Japan). Immunolocalization of IL‐33 or CXCL10 was performed by histochemical staining using primary goat anti‐mouse‐IL‐33 and anti‐CXCL10 (R&D Systems, Minneapolis, MN) and secondary horseradish peroxidase‐conjugated rabbit anti‐goat antibody for IL‐33 (Dako, Markham, Ont., Canada) and OmniMap anti‐Rabbit‐horseradish peroxidase (RUO) for CXCL‐10 followed by haematoxylin counterstaining in a Ventana machine (Ventana Medical Systems, Inc. Tucson, AZ), as previously described.36 Counting of necrotic areas and inflammatory foci was carried out on liver areas of 15–30 mm2 using the NDP.2 view image analysis software (Hamamatsu Photonics K.K., Japan).
Bleau C., Burnette M., Filliol A., Piquet‐Pellorce C., Samson M, & Lamontagne L. (2016). Toll‐like receptor‐2 exacerbates murine acute viral hepatitis. Immunology, 149(2), 204-224.
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5

Glycemia and Liver Enzyme Monitoring

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All animals were monitored daily and weighed once a week. Glycemia tests using glucometer (Freestyle Optium Neo, Abbott, Chicago, IL, USA) were performed after weaning and at slaughtering with submandibular blood collection using a lancet (#GR-3.5mm, Goldenrod Animal Lancet, Bioseb Lab).
Serum alanine aminotransferase (ALT) transaminase levels were measured according to the International Federation of Clinical Chemistry and Laboratory Medicine primary reference procedures using an Olympus AU2700 Autoanalyser (Olympus Optical).
Simoes Eugénio M., Farooq M., Dion S., Devisme C., Raguenes-Nicol C., Piquet-Pellorce C., Samson M., Dimanche-Boitrel M.T, & Le Seyec J. (2020). Hepatocellular Carcinoma Emergence in Diabetic Mice with Non-Alcoholic Steatohepatitis Depends on Diet and Is Delayed in Liver Exhibiting an Active Immune Response. Cancers, 12(6), 1491.
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6

Fasting Blood Analyses in Patients

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Venous blood samples were drawn from patients and control subjects between 08:00 and 09:00 h after overnight fasting for biochemical analyses. The samples were centrifuged for 15 min at 4000 g, aliquoted, and immediately frozen at -80°C for analyses. Fasting plasma glucose, total cholesterol, triglyceride, and high-density lipoprotein cholesterol (HDL-C) levels were measured by the enzymatic colorimetric method with an Olympus AU2700 auto analyser using reagents from Olympus Diagnostics (GmbH, Hamburg, Germany). Low-density lipoprotein cholesterol (LDL-C) was calculated by Friedewald's formula [16] . The serum basal insulin level, total testosterone, follicle stimulating hormone (FSH), and luteinizing hormone (LH) were measured by the chemiluminescence method with a Unice lDXI 800 Access Immunoassay System (Miami, FL, ABD). Insulin sensitivity was calculated by using the homeostatic model assessment-insulin resistance (HOMA-IR) by the following formula:
Demirci I., Haymana C., Candemir B., Meric C., Yuksel B., Eser M., Akın O., Akın S., Ersoz Gulcelik N, & Sonmez A. (2021). Triglyceride-glucose index levels in patients with congenital hypogonadotropic hypogonadism and the relationship with endothelial dysfunction and insulin resistance. Endokrynologia Polska, 72(3).
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7

Biochemical Analysis of Fasting Blood

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For biochemical analyses, all blood samples were collected from the antecubital vein, between 08:00 and 09:00 after an overnight fasting. The samples were centrifuged for 15 minutes at 4000 rpm, aliquoted, and immediately frozen at -80°C for analyses. Fasting blood glucose (FBG), total cholesterol, triglyceride (TG), and high-density lipoprotein cholesterol (HDL-C) levels were measured by the enzymatic colorimetric method with an Olympus AU2700 auto analyser using reagents from Olympus Diagnostics (GmbH, Hamburg, Germany). Low-density lipoprotein cholesterol (LDL-C) was calculated by Friedewald's formula [21] . The serum basal insulin, testosterone, FSH, and LH were measured by the electrochemiluminescence method with the UnicelDXI 800 Access Immunoassay System (Miami, FL, ABD). Insulin sensitivity was calculated
Haymana C., Sonmez A., Aydogdu A., Tapan S., Basaran Y., Meric C., Baskoy K., Taslipinar A., Yilmaz M.I, & Azal O. (2017). Effect of testosterone replacement therapy on vitamin D and FGF-23 levels in congenital hypogonadism. Endokrynologia Polska, 68(3).
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