Klra8

Mice were bred at UCLA and Memorial Sloan Kettering Cancer Center in accordance with the guidelines of the institutional Animal Care and Use Committee (IACUC). The following mouse strains were used this study: C57BL/6 (CD45.2) (Jackson Labs, #000664), B6.SJL (CD45.1) (Jackson Labs, #002114), Rag2^−/− (Jackson Labs #008449), Klra8^−/− (Ly49H-deficient), Zbtb32^−/−, and Il12rb2^−/−x Il18ra1^−/− (MSKCC, J.C. Sun). Experiments were conducted using 7–8 week old age- and gender-matched mice in accordance with approved institutional protocols.
Generation of mixed bone marrow chimeric mice was performed as previously described^{18 (link)}. Parabiosis surgery was performed as previously described^{12 (link), 15 (link), 16 (link), 17 (link)}. Intravascular labeling of lymphocytes of experimental mice was performed by injecting (i.v.) 2.5 μg of fluorophore-conjugated CD45 (30-F11) and euthanized 3 minutes later.

All mice used in this study were bred at the Memorial Sloan Kettering Cancer Center in accordance with the guidelines of the Institutional Animal Care and Use Committee. The following strains were used, all on the C57BL/6 genetic background: C57BL/6 (CD45.2; the Jackson Laboratory), B6.SJL (CD45.1; Taconic), NKp46^iCre (49 (link)), Runx1^fl/fl (50 (link)), Runx3^fl/fl and Cbfb^fl/fl (51 (link)), Stat4^−/− (52 (link)), Klra8^−/− [Ly49h^−/− (53 (link))], Ubc^ERT2-Cre, and Rag2^−/− × Il2rg^−/− (the Jackson Laboratory). Generation of mixed BMC mice and adoptive transfer studies were performed as previously described (54 (link)).
In some experiments, Rag2^−/− × Il2rg^−/− recipient mice or BMC mice were injected intraperitoneally with 4-hydroxytamoxifen (1 mg per day) dissolved in corn oil (Sigma) or corn oil alone as a control for five consecutive days. Experimental mice were infected by intraperitoneal injection of 7.5 × 10² plaque-forming units (PFU) of in vivo passaged, salivary gland–derived MCMV (Smith Strain). Mice infected with a lethal dose of MCMV were intraperitoneally injected with 4 × 10⁴ PFU of MCMV. Experiments were conducted using age- and gender-matched mice in accordance with approved institutional protocols.