Viptm1 cre zjh j

All experiments were approved by the University of Michigan Institutional Animal Care and Use Committee and were in accordance with NIH guidelines for the care and use of laboratory animals. Animals were kept on a 12 hour day/night cycle with ad libitum access to food and water. VIP-IRES-Cre mice (Vip^tm1(cre)Zjh/J, Jackson Laboratory, stock # 010908) (Taniguchi et al., 2011 (link)) were crossed with Ai14 reporter mice (B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J, Jackson Laboratory, stock # 007914) (Madisen et al., 2010 (link)) to yield F1 offspring that expressed the fluorescent protein tdTomato in VIP neurons. For control experiments, C57BL/6J mice (Jackson Laboratory, stock # 000664) were used. Because mice on the C57BL/6 background undergo age-related hearing loss, experiments were restricted to an age range where hearing loss should be minimal or not present (Zheng et al., 1999 (link)). This age range was P70 or less for all mice except for three C57BL/6J mice used for electrophysiology experiments that were aged P89, P90, and P113.

All experimental procedures were carried out following the guidelines of the Animal Care and Experimentation Committee of the Institute of Experimental Medicine of the Hungarian Academy of Sciences, and the Cold Spring Harbor Laboratory Institutional Animal Care and Use Committee, in accordance with the Hungarian, EU, and National Institutes of Health regulations (reference number: PEI/001/194-4/2014). We used male and female adult (6–24 week-old) VIP-Cre, PV-Cre, and Thy-1-Cre mice (Vip^tm.1(cre)Zjh/J, B6.129P2-Pvalb^tm1(cre)Arbr/J, FVB/N-Tg(Thy1-cre)1Vln/J, The Jackson Laboratory) housed in small groups of 2–4 under controlled temperature and humidity conditions. They were kept on a reverse light cycle, and during the training and the experimental period the water consumption of the VIP-Cre and PV-Cre mice was restricted to 1 ml/day after recovering from surgery. The mice had ad libitum access to food.

All experiments were approved by the University of Michigan Institutional Animal Care and Use Committee and were in accordance with NIH guidelines for the care and use of laboratory animals. Animals were kept on a 12-h day/night cycle with ad libitum access to food and water. VIP-IRES-Cre mice (Vip^{TM 1(cre)Zjh}/J, Jackson Laboratory, stock # 010908) (Taniguchi et al., 2011 (link)) were crossed with Ai14 reporter mice (B6.Cg-Gt(ROSA)26Sor^{TM 14(CAG–tdTomato)Hze}/J, Jackson Laboratory, stock #007914) (Madisen et al., 2010 (link)) to yield F1 offspring that expressed the fluorescent protein tdTomato in VIP neurons. Because mice on the C57BL/6J background undergo age-related hearing loss, experiments were restricted to mice aged P30 – P85, an age range where hearing loss should be minimal or not present (Zheng et al., 1999 (link)).

All experiments were carried out in accordance with the UK Animals (Scientific Procedures) Act of 1986, with local ethical approval provided by the Medical Research Council Laboratory of Molecular Biology Animal Welfare Ethical Review Board (LMB AWERB) and overseen institutionally by designated animal welfare officers (NACWOs). VIP^Cre mice (Viptm1(cre)Zjh/J) were purchased from the Jackson Laboratory (Bar Harbor, Maine, USA). VPAC2^Cre mice (Tg(Vipr2-cre)KE2Gsat/Mmucd) were purchased from GENSAT (Gene Expression in the Nervous System Atlas) project (Rockefeller University, New York City, USA). R26 floxed STOP EYFP (B6.129×1-Gt(ROSA)26Sortm1(EYFP)Cos/J) mice were kindly provided by A. McKenzie, MRC Laboratory of Molecular Biology, Cambridge, UK. Cry1-null and Cry2-null animals were derived from founders kindly provided by G. van der Horst, Erasmus University Medical Centre, Rotterdam, NL. Per2::Luciferase mice were kindly provided by J. S. Takahashi, University of Texas Southwestern Medical Centre, Dallas, USA. All lines were maintained on a C57BL/6J background with required genotypes bred in-house by intercrossing the lines.