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Ab1839

Manufactured by Merck Group
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Sourced in Germany

AB1839 is a laboratory equipment product manufactured by Merck Group. It is designed for general laboratory purposes. The core function of AB1839 is to provide a reliable and versatile tool for researchers and scientists working in various fields of study.

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Lab products found in correlation

Mab1004, by Merck Group (4 mentions) Mab1044, by Merck Group (2 mentions) A0398, by Agilent Technologies (1 mentions) Ebio7979, by Thermo Fisher Scientific (1 mentions) E0413, by Agilent Technologies (1 mentions) E0353, by Agilent Technologies (1 mentions) A0452, by Agilent Technologies (1 mentions) Goat anti rabbit hrp, by Jackson ImmunoResearch (1 mentions) Type 1500 multiskan spectrum microplate reader, by Thermo Fisher Scientific (1 mentions)

4 protocols using ab1839

1

Immune Markers in Tissue Samples

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The following antibodies were used: polyclonal rabbit antibody against human myeloperoxidase (MPO) (A0398, Dakocytomation, Glostrup, Denmark) and cluster of differentiation 3 (CD3 (A0452, Dakocytomation, Glostrup, Denmark), monoclonal rabbit antibody against human fork head box P3 (FoxP3) (clone eBio7979, 14-7979-82, eBioscience, San Diego, CA, USA), ovine interleukin-6 (IL-6) (MAB1004, Millipore, Darmstadt, Germany), and ovine IL-8 (MAB1044 Millipore, Darmstadt, Germany). Antibodies against intestinal fatty acid binding protein (I-FABP) were kindly provided by the Department of Surgery, Maastricht University Medical Centre, the Netherlands. Secondary antibodies were the following: biotin-conjugated rabbit antimouse (E0413, DakoCytomation, Glostrup, Denmark), swine antirabbit (E0353, DakoCytomation, Glostrup, Denmark), and peroxidase-conjugated goat antirabbit (111-035-045, Jackson, West Grove, PA, USA). Detection antibodies against IL-6 (AB1839, Millipore, Darmstadt, Germany) and IL-8 (AB1840, Millipore, Darmstadt, Germany) were used.
van Gorp C., de Lange I.H., Spiller O.B., Dewez F., Cillero Pastor B., Heeren R.M., Kessels L., Kloosterboer N., van Gemert W.G., Beeton M.L., Stock S.J., Jobe A.H., Payne M.S., Kemp M.W., Zimmermann L.J., Kramer B.W., Plat J, & Wolfs T.G. (2019). Protection of the Ovine Fetal Gut against Ureaplasma-Induced Chorioamnionitis: A Potential Role for Plant Sterols. Nutrients, 11(5), 968.
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2

Fetal Plasma Cytokine Quantification

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Concentrations of fetal plasma cytokines were measured as previously described using sandwich enzyme-linked immunosorbent assays (ELISA) employing the following antibodies: IL-1β (coating antibody, rabbit anti-ovine IL-1β. Guinea pig anti-ovine IL-1β primary antibody [Both Seven Hills Bioreagents, Cincinnati, OH.]); IL-6 (coating antibody, mouse anti-ovine IL-6 [MAB 1004, Millipore, Billerica, MA.] Rabbit anti-ovine IL-6 primary antibody [AB1839, Millipore, Billerica, MA.] The detection antibody for all the assays was an appropriate specific HRP-conjugated antibody. The detection limits and the dynamic range of measurement were: IL-1β—0.2–12.0 ng/ mL, IL-6—0.2–12.0 ng/mL. The correlation coefficient was 0.94–0.99 for all assays. Plasma haptoglobin levels were measured ELISA following the manufacturer’s instruction (catalog no. E-35HPT; ICL, Portland, OR).
Maneenil G., Kemp M.W., Kannan P.S., Kramer B.W., Saito M., Newnham J.P., Jobe A.H, & Kallapur S.G. (2015). Oral, Nasal and Pharyngeal Exposure to Lipopolysaccharide Causes a Fetal Inflammatory Response in Sheep. PLoS ONE, 10(3), e0119281.
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3

Fetal Plasma IL-6 Quantification

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The pro-inflammatory cytokine IL-6 was measured in fetal plasma as marker for systemic inflammation using a sheep-specific sandwich enzyme-linked immunosorbent assay (ELISA). Briefly, a mouse-anti-ovine monoclonal antibody (MAB1004, Millipore, Darmstadt, Germany, working concentration 1:200) was the coating antibody. Plasma samples (100 μL) were loaded per well in duplicate and incubated for 2 hours at room temperature. Incubation with the detection antibody (rabbit-anti-ovine IL-6, AB1839, Millipore, Darmstadt, Germany, working concentration 1:500) was performed for 60 minutes, followed by incubation for 30 minutes with 100 μL of a goat-anti-rabbit-HRP (Jackson ImmunoResearch Laboratories Inc, West Grove, PA, USA, working concentration 1:500). After washing, incubation with 3,3′,5,5′-tetramethylbenzidine (TMB) substrate solution for 15 minutes. The reaction was stopped by addition of 50 μL 2N sulfuric acid. The optical density (OD) was measured using a micro-plate reader at 450 nm. Concentrations were expressed relative to a standard curve of ovine IL-6 recombinant protein (ImmunoChemistry Technologies, Bloomington, MN, USA).
Gussenhoven R., Ophelders D.R., Kemp M.W., Payne M.S., Spiller O.B., Beeton M.L., Stock S., Cillero-Pastor B., Barré F.P., Heeren R.M., Kessels L., Stevens B., Rutten B.P., Kallapur S.G., Jobe A.H., Kramer B.W, & Wolfs T.G. (2017). The paradoxical effects of chronic intra-amniotic Ureaplasma parvum exposure on ovine fetal brain development. Developmental neuroscience, 39(6), 472-486.
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4

Ovine Cytokine ELISA Assay

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Levels of the pro-inflammatory cytokines interleukin (IL)-6 and IL-8 were measured in fetal plasma as markers for systemic inflammation using ovine-specific sandwich enzyme-linked immunosorbent assays (ELISA) as previously described [8 ].
In short, a 96-wells plate was coated with a monoclonal mouse-anti IL-6 (Millipore Cat# MAB1004, working concentration 1:200) or IL-8 (Millipore Cat# MAB1044, working concentration 1:200) and incubated overnight at 4 °C. The standard curve and serum samples were diluted in PBS + 0.1% BSA in 1:1 or 1:80, respectively, for IL-6 and IL-8. Incubation with the detection antibody rabbit-anti-ovine IL-6 (Millipore Cat# AB1839, working concentration 1:500) or IL-8 (AB1040, Millipore, working concentration 1:500) was performed for 1 h, followed by incubation with a HRP-labeled antibody (Jackson ImmunoResearch Labs Cat# 111-035-045, working concentration 1:500). Next, incubation with 3,3′5,5′-tetramethylbenzidine (TMB) substrate solution was done for 10 (IL-6) or 2,5 (IL-8) minutes. The reaction was stopped by addition of H2SO4, and the optical density (OD) was measured at 450 nm in a Thermo Electron Type 1500 Multiskan Spectrum Microplate Reader. Concentrations were expressed relative to a standard curve of recombinant ovine IL-6 or IL-8 (ImmunoChemistry Technologies, Bloomington, MN, USA).
Gussenhoven R., Westerlaken R.J., Ophelders D.R., Jobe A.H., Kemp M.W., Kallapur S.G., Zimmermann L.J., Sangild P.T., Pankratova S., Gressens P., Kramer B.W., Fleiss B, & Wolfs T.G. (2018). Chorioamnionitis, neuroinflammation, and injury: timing is key in the preterm ovine fetus. Journal of Neuroinflammation, 15, 113.
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