Ncr nu nu male mice

Approximately 10⁶ PSMA+ CWR22Rv1 cells in 50/50 complete medium and matrigel matrix were implanted in the right shoulder of athymic NCr-nu/nu male mice (approximately 8 weeks old) from Charles River (Hollister, CA). Four weeks after implantation, mice with tumors reaching 150 to 300mm³ were anesthetized by isoflurane inhalation and injected with [¹⁸F]5, 150–200 μCi in 250 μL of saline, in a tail vein. The anesthetized mice were imaged with 10 min acquisition using a microPET/CT imaging system (Inveon, Siemens, Knoxville, TN) at 1 h and 4 h post injection. PET imaging data were acquired in list mode and reconstructed with the iterative OSEM 2-D reconstruction algorithm provided by the manufacturer.

Six to eight-week-old athymic NCr-nu/nu male mice were purchased from Charles River (Hollister, CA). All animal experiments were conducted in accordance with the UCSF IACUC-approved protocol. [¹⁸F]5 was evaluated in vivo using mouse xenografts.

All animal experiments were conducted in accordance with the UCSF IACUC approved protocol. Approximately 10⁶ CWR22Rv1 PSMA(+) or PC3 PSMA(−) cells in 50:50 mixture of complete medium and matrigel matrix were implanted in the right shoulder of athymic NCr-nu/nu male mice (approximately 8 weeks old) from Charles River (Hollister, CA). Approximately 4 weeks after implantation, animals with tumors reaching 150–300 mm³ were anesthetized by isoflurane inhalation and were administered with [¹⁸F]4, [¹⁸F]5, or [¹⁸F]6 at 200–250 μCi in 250 μL of saline through tail vein injection. The resulting animals were imaged with 10 min acquisition by a microPET/CT imaging system (Inveon, Siemens, Germany) at 2 h postinjection. PET imaging data were acquired in list mode and reconstructed with the iterative OSEM 2-D reconstruction algorithm provided by the manufacturer.