For CD8+ T cells depletion, 100 μg/mouse purified anti-mouse CD8 antibody (Biolegend, clone: 53-6.7, Cat# 100735) was injected via lateral tail vein 24 hours before the last vaccination. For NK depletion, 50 μg/mouse purified anti-Asialo-GM1 Antibody (Biolegend, clone: Poly21460, Cat# 146002) was injected through lateral tail vein 24 hours before vaccination.
Anti asialo gm1 antibody
Manufactured by BioLegend
Sourced in Germany
Anti-Asialo-GM1 Antibody is a lab reagent used in research applications. It is a polyclonal antibody that binds to asialo-ganglioside-GM1, a glycolipid expressed on the surface of natural killer cells and some T cells.
Automatically generated - may contain errors
Lab products found in correlation
Anti mouse cd8 antibody, by BioLegend (1 mentions)
Anti cd4 antibody clone gk1, by BioXCell (1 mentions)
Anti mouse cd8a antibody clone 2, by BioXCell (1 mentions)
Hepes, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Anti pd l1 10f 9g2, by BioXCell (1 mentions)
Anti ifnar1 mar1 5a3, by BioXCell (1 mentions)
Lenticrispr v2 plasmid, by Addgene (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Gsi ro4929097, by Selleck Chemicals (1 mentions)
Bortezomib, by Selleck Chemicals (1 mentions)
6 protocols using anti asialo gm1 antibody
1
CD8+ T cells and NK depletion
2
Depletion of immune cells for anti-tumor immunity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anti-mouse CD8a antibody (clone 2.43, BioxCell) and anti-CD4 antibody (clone GK1.5, BioxCell) were used to deplete CD8+ T cells and CD4+ T cells as described in a previous study (15 (link)). Briefly, mice with tumors were treated with LMB-100 (i.p., 60 μg/dose) on days 5 and 9, and each antibody or isotype control (rat IgG2b) was administered (i.p., 200 μg/dose) on days 5, 9, and 13. NK cells were depleted with anti-asialo-GM1 antibody (BioLegend, Clone Poly21460) on days 4, 6, and 11 as described previously (i.p., 30 μl with 170 μl PBS/dose) (41 (link)). B cell depletion was performed as previously described (42 (link)). Mice were injected with a mixture of monoclonal antibodies: rat anti-mouse CD19 (clone 1D3, i.p., 150 μg), rat anti-mouse B220 (clone RA3-6B2, i.p., 150 μg), and mouse anti-mouse CD22 (clone CY34, i.p., 150 μg) on days 2, 10, and 18; after 48 h of each injection, the mice were further injected with anti-rat kappa immunoglobulin light chain antibody (clone MAR 18.5, i.p., 150 μg). To check whether the antibody treatment impaired the development of anti-tumor immunity, the mice with CRs were re-challenged with 2 × 106 AB1-L9 cells about 1.5 mo after the initial cell inoculation.
3
Mouse TNF-α Antibody Purification and CpG ODN Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Recombinant LEAF (Low Endotoxin, Azide Free) purified anti-mouse TNF-α IgG1 κ antibody (Cat # 510804) and mouse isotype control IgG1κ were purchased from BioLegend. Phosphorothioate CpG ODN 1555 (GCTAGACG TTAGCGT) was synthesized at the CBER core facility. All batches of ODN were tested for endotoxin content using Limulus Amebocyte Lysate assay and had <0.1 unit of endotoxin per milligram of ODN. DMEM, phenol red free EMEM, 2X EMEM, penicillin streptomycin solution (100×), L-glutamine solution (100×), trypsin (0.25%)-EDTA(1 mM) and HEPES were purchased from Invitrogen. FBS was purchased from HyClone. Anti-asialo GM1 antibody (Cat # 146002) was purchased from Biolegend and used for depleting NK cells in B6-WT mice. The antibody solution provided by the manufacturer was a gamma globulin fraction of polyclonal serum. Sindbis virus NSV rabbit polyclonal antibody and Sindbis E2 (SV127) mouse monoclonal antibody (28 (link)) were kindly provided by Prof. Diane E. Griffin (Johns Hopkins University, Baltimore).
4
Generation and Utilization of PD-L1 Knockout Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The 293F cells were kindly provided by Dr. Ting Xu (Alphamab, Suzhou, Jiangsu, China) and grown in SMM 293-TI medium (M293TI, Sino Biological). A20, MC38, B16F10, and L929 cell lines were purchased from ATCC (Manassas, VA). PD-L1-deficient MC38 and A20 cell lines were generated by CRISPR/Cas9 technology. The guide sequences (5ʹ-GACTTGTACGTGGTGGAGTA-3ʹ) and EGFP gene were cloned into lentiCRISPR v2 plasmid (Addgene, catalog 52961). Thirty hours post transfection, EGFP-positive cells were sorted and subcloned by flow cytometry. Identified through FACS staining, cell clones without PD-L1 or EGFP expression were used for the following studies.
Anti-PD-1 blockade Ab (4H2) was from Bristol-Myers Squibb (Redwood City, CA). Anti-PD-L1 (10F.9G2) and anti-IFNAR1 (MAR1–5A3) antibodies were purchased from BioXCell (West Lebanon, NH). Anti-CD8 (TIB210) and anti-CD4 depleting Ab (GK1.5) were produced in house. Anti-Asialo-GM1 antibody were purchased from Biolegend (San Diego, CA).
Anti-PD-1 blockade Ab (4H2) was from Bristol-Myers Squibb (Redwood City, CA). Anti-PD-L1 (10F.9G2) and anti-IFNAR1 (MAR1–5A3) antibodies were purchased from BioXCell (West Lebanon, NH). Anti-CD8 (TIB210) and anti-CD4 depleting Ab (GK1.5) were produced in house. Anti-Asialo-GM1 antibody were purchased from Biolegend (San Diego, CA).
5
Antibody-Mediated Suppression of HBV
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
5 × 1010 GC of AAV8-HBV1.3 was given to each mouse via tail vein injection. Three days later, 7 mice (3 female, 4 male) were injected intraperitoneally with an anti-HBc monoclonal antibody (100 μg/mouse). Eight mice (4 female, 4 male) were injected with a purified control IgG (100 μg/mouse). Another 4 mice (2 female, 2 male) were treated with the anti-HBc mAb and a NK cell depletion antibody (purified anti-Asialo-GM1 Antibody, clone# 146002, BioLegend). The anti-HBc mAb was a kind gift from Quan Yuan from Xiamen University. Peripheral blood was collected at multiple timepoints after antibody treatment. Anti-HBc IgG responses and serum HBsAg concentrations were monitored by ELISA assays.
6
Anti-BCMA ADC HDP-101 Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The anti-BCMA ADC HDP-101 (24 (link), 25 (link), 26 (link)) and control reagents were provided by Heidelberg Pharma AG (Ladenburg, Germany), while anti-Asialo-GM1 antibody was obtained from BioLegend (San Diego, CA). Bortezomib and the gamma secretase inhibitor (GSI) RO4929097 (27 (link)) were purchased from Selleck Chemical (Houston, TX), and drug stock solutions were prepared in dimethyl-sulfoxide (Fisher Scientific; Pittsburgh, PA) and stored at −20°C.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!