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Antibody for glyceraldehyde 3 phosphate dehydrogenase gapdh

Manufactured by Merck Group
Sourced in United States

The Antibody for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a laboratory tool used to detect and quantify the GAPDH protein in biological samples. GAPDH is a key enzyme involved in the glycolysis pathway, making it a widely used reference or housekeeping protein for various applications in cell biology and molecular biology research.

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4 protocols using antibody for glyceraldehyde 3 phosphate dehydrogenase gapdh

1

Coptisine and MFG-E8 Antibody Assay

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Coptisine was purchased from Sigma-Aldrich (St. Louis, MO, USA). MFG-E8 antibody was purchased from Santa Cruz (USA). Antibodies for PI3K, AKT, p-AKT, MMP-9, MMP-2, E-cadherin, snail, N-cadherin and vimentin were purchased from Abcam (CA, USA). Antibody for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was from Sigma-Aldrich (St. Louis, MO, USA). CCK8 kit was purchased from Biosharp (Hefei, China). All other chemicals were from Sigma-Aldrich.
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2

Parecoxib-Induced EMT Pathway Study

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ParecoxibNa was purchased from Pfizer (Pfizer Inc., New York, USA). Antibodies for ELMO3, COX-2, E-cadherin, Snail, N-cadherin, and Vimentin were purchased from Abcam (CA, USA). Antibody for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was from Sigma-Aldrich (St. Louis, MO, USA). CCK8 kit was purchased from Biosharp (Hefei, China).
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3

Chromatographic Analysis of Signaling Proteins

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Chromatographic grade methanol and acetonitrile were purchased from Merck (Darmstadt, Germany). Formic acid, ethyl acetate and other chemicals were purchased from commercial sources and of analytical grade. Rabbit monoclonal to Bad, rabbit monoclonal to Bcl-xl, rabbit monoclonal to Bax, rabbit polyclonal to Bcl-2, rabbit polyclonal to p-NF-κB p50, rabbit polyclonal to p-NF-κB p65, and rabbit monoclonal to p-IκB were purchased from Abcam (Cambridge, United Kingdom). Antibodies for mouse monoclonal to NLRP3 were acquired from Adipogen International (San Diego, CA, United States). Antibody for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were acquired from Sigma-Aldrich (St. Louis, MO, United States). Evans blue (EB) was purchased from Sigma Chemical Co., Ltd. (St. Louis, MO, United States). All other reagents were obtained from Sigma-Aldrich (St. Louis, MO, United States).
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4

Celastrol Inhibits TGF-β1 Signaling

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Celastrol was obtained from Sigma-Aldrich (St Louis, MO, USA). The primary antibodies of TGF-β1, Smad2/3, p-Smad2/3, TGFβRI, TGFβRII, and Smad4 were purchased from Abcam (Cambridge, UK). Cell counting kit-8 (CCK-8) was purchased from Biosharp (Hefei, China). Antibody for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and other reagents were purchased from Sigma-Aldrich.
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