Jem 1200 ex 2 microscope

The scaffold morphology was assessed by means of scanning electron microscope (SEM) analysis (Tescan, Mira3XMU, Brno, Czech Republic). The samples were sputtered with graphite. Electrospun nanofiber diameters were assessed by image analysis software (ImageJ, ICY, Institut Pasteur, Paris, France). Moreover, the inclusion of the CuO nanoparticles into the fibrous structure was evaluated by means of SEM-EDX, recording an EDX spectrum, and also by means of transmission electron microscope (TEM) analysis (JEOL JEM-1200 EX II microscope; CCD camera Olympus Mega View G2, with 1376 × 1032-pixel format, Tokyo, Japan; operating HV at 100 kV, magnification 100 k). For this purpose, a thin layer of fiber was electrospun directly onto the TEM grids (formavar/carbon 300-mesh Cu, Agar Scientific, Monterotondo (RM), Italy).
The wettability of the electrospun fibers was assessed with a contact angle meter (DMe-211 Plus; FAMAS software, Kyowa, Osaka, Japan). The droplet shape (0.4 µL of PBS) was captured through the CCD camera at 1 s after the droplet touched the scaffold surface.

The scaffolds’ morphology
was assessed with a scanning electron
microscope (SEM) (Tescan, Mira3XMU, Brno, Czech Republic) by sputtering
the samples with graphite. The nanofibers’ dimensions were
evaluated by an image analysis software (ImageJ, ICY, Institut Pasteur,
Paris, France). For this purpose, to ensure that the measured fibers
were randomly chosen and representative of the whole scaffold, three
different images were used, and 30 analyses each were performed, with
a final total of 90 analyses.
The incorporation of the CeO₂ nanoparticles into the fibrous matrix was assessed with a
transmission electron microscope (TEM) (JEOL JEM-1200 EX II microscope;
CCD camera Olympus Mega View G2 with 1376 × 1032 pixel format,
Tokyo, Japan; operating HV at 100 kV; magnification 100k). In this
regard, the fibers were electrospun onto the grids (formavar/carbon
300 mesh Cu, Agar Scientific, Monterotondo (RM), Italy).
The
wettability of the scaffolds was tested by contact angle measurements
(DMe-211 Plus; FAMAS software, Kyowa, Osaka, Japan).

TEM analysis (JEOL JEM-1200 EX II microscope; CCD camera Olympus Mega View G2 with 1376 × 1032 pixel format, Tokyo, Japan) was performed to assess the HP inclusion in the electrospun nanofibrous structure (operating HV at 100 kV; magnifications: 15k, 25k, 50k). For this purpose, a thin layer of fiber was electrospun directly onto the TEM grids (formavar/carbon 300 mesh Cu, Agar Scientific, Monterotondo (RM), Italy) and cross-linked by heating, as previously described before the analysis.
Scaffold morphology was characterized by means of SEM analysis (Tescan, Mira3XMU, platinum sputtering, Brno, Czech Republich). Electrospun nanofiber diameters, pore size, and degree of orientation (alignment) were assessed by image analysis software (ImageJ, ICY, Institut Pasteur, Paris, France).
The wettability of the electrospun fibers was assessed with a contact angle meter (DMe-211 Plus; FAMAS software, Kyowa, Osaka, Japan). The droplet shape (0.4 µL of PBS) was captured through the CCD camera at 1 s after the droplet touched the scaffold interface.
FT-IR analysis was carried out by means of an infrared imaging microscope (FT-IR, Spectrum BX, Perkin Elmer, Milano, Italy). The infrared spectra were acquired in the range 4000–400 cm⁻¹. The measurements were performed on cutouts of the random crosslinked scaffolds (R-B, R-0.1 HP, R-0.5 HP), 5 × 5 mm², 10 µm thick.