PBMCs from subjects were resuspended in PBS buffer and were incubated with directly conjugated antibodies for 30 min at 4°C. The cells were washed with 1× PBS before flow cytometry analysis. Antibodies used included anti-human CD3-BV786, CD19-BVAPC-H7, CD16-BV711, CD69 PE-CF594, CD57-BV421, PD-1-APC, CD70-PE, CD160-AF488, 2B4-AF700, CTLA-4-PE, TIM-3-FITC, 7-AAD (BD Biosciences, San Diego, CA, USA), CD56-BV510, BTLA-PE-CY7, CD39-APC (BioLegend, San Diego, CA, USA), TIGIT-PE-Cy7 (Ebioscience, San Diego, CA, USA), along with the corresponding isotype controls. NK cells were gated as CD3−CD14−CD19−CD56+CD16+, CD3−CD14−CD19−CD56+CD16−, or CD3−CD14−CD19−CD56−CD16+. Data were acquired with the LSR Fortessa flow cytometer (BD Biosciences) and analyzed with FlowJo software version 10.5 (Tree Star, Ashland, OR, USA).
Cd57 bv421
Manufactured by BD
Sourced in United States
The CD57-BV421 is a monoclonal antibody conjugated with the Brilliant Violet 421 fluorochrome. It is designed for the detection and enumeration of CD57-positive cells by flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Tigit pe cy7, by Thermo Fisher Scientific (2 mentions)
Cd160 af488, by BD (2 mentions)
Lsrfortessa, by BD (1 mentions)
7 aad, by BD (1 mentions)
Ctla 4 pe, by BD (1 mentions)
Cd56 bv510, by BioLegend (1 mentions)
Pd 1 apc, by BD (1 mentions)
Cd70 pe, by BD (1 mentions)
2b4 af700, by BD (1 mentions)
Cd16 bv711, by BD (1 mentions)
Cd69 pe cf594, by BD (1 mentions)
Tim 3 fitc, by BD (1 mentions)
Cd39 apc, by BioLegend (1 mentions)
Cd133 apc, by Miltenyi Biotec (1 mentions)
Lsrfortessa cell analyzer, by BD (1 mentions)
Nkg2d apc, by Miltenyi Biotec (1 mentions)
Cd16 ecd, by Beckman Coulter (1 mentions)
Cd158e1 bv421, by BioLegend (1 mentions)
Nkp30 pe, by BioLegend (1 mentions)
Nkp44 pe, by Beckman Coulter (1 mentions)
3 protocols using cd57 bv421
1
Comprehensive NK cell phenotyping
2
NK Cell Surface Marker Profiling
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NK3.3-LTV cells were analyzed for changes in cell surface marker expression compared to non-immortalized NK3.3 cells. All cells (1 x 105 cells per antibody) were centrifuged at 300 g x 5 min, media aspirated, and 1.5 µL single antibody + 20 µL staining buffer (phosphate buffered saline (PBS) + 1% bovine serum albumin + 1% sodium azide) were added to cells. Sample tubes were incubated for 30 min on ice, followed by addition of 200 µL staining buffer and incubated for another 10 min on ice. Samples were analyzed using LSRFortessa Cell Analyzer (BD Biosciences, Franklin Lakes, NJ). Data were analyzed using FlowJo software. Antibodies used include: NKp46 PE/Cy7 (#331915, BioLegend, San Diego, CA); NKp30 PE (#325207, BioLegend); NKp44 PE (#IM3710, Beckman Coulter, Brea, CA); CD94 PE (#130-098-974, Miltenyi Biotec, Auburn, CA); NKG2D APC (#120-003-706, Miltenyi Biotec); CD161 APC-Alexa Fluor750 (#B30630, Beckman Coulter); CD158e1 BV421 (#312713, BioLegend); CD158a,h PE/Cy7 (#A66899, Beckman Coulter); CD244 PerCp Cy5.5 (#B21171, Beckman Coulter); CD16 ECD (#A33098, Beckman Coulter); CD133 APC (#130-098-829, Miltenyi Biotec); CD57 BV421 (#563896, BD Biosciences); CD158 FITC (#339503, BioLegend).
3
Comprehensive Immune Phenotyping of PBMCs
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PBMCs from healthy subjects were resuspended in PBS buffer and were incubated with directly conjugated antibodies for 30 min at 4°C. The cells were washed with 1× PBS before flow cytometry analysis. Antibodies used included anti-human CD3-BV786 or CD3-BUV737, CD8-BV510 or CD8-BUV395, CD160-AF488, CD45RA-AF700, CD28-APC, CD95-PE, CD57-BV421, PD-1-BV711, TIM-3-BV650 (BD Biosciences, San Diego, CA, USA), CD4-APC-Fire750, CD244-PE-D594, CCR7-BV421, HLA-DR-AF700, CD38-BV421, CD28-BV711, CD27-BV650, KLRG-1-APC-Fire750, CD95-PE-CY7 (BioLegend, San Diego, CA, USA), TIGIT-PE-Cy7, LAG-3-APC (Ebioscience, San Diego, CA, USA) and the corresponding isotype controls. Data were acquired with the LSR Fortessa flow cytometer (BD Biosciences) and analyzed with FlowJo software version 10.5 (Tree Star, Ashland, OR, USA). More information about antibodies is listed in the Supplementary Material Table S2
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