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Permount mounting media

Manufactured by Electron Microscopy Sciences
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Permount is a permanent mounting medium used for the preparation of microscope slides. It is designed to preserve and protect mounted specimens while providing a clear, optically suitable medium for long-term storage and observation.

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Lab products found in correlation

Direct red 80, by Merck Group (2 mentions) Bz x810 microscope, by Keyence (2 mentions) Fast green fcf, by Merck Group (2 mentions) P6744 1ga, by Merck Group (2 mentions) Protein block serum free, by Agilent Technologies (1 mentions) α sma, by Abcam (1 mentions) Stat1, by Cell Signaling Technology (1 mentions)

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Permount (21 citations) Mounting media (6 citations)

6 protocols using permount mounting media

1

TRAP Staining of Decalcified Bone

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Decalcified bone sections were de-paraffinized in xylene, rehydrated through an ethanol gradient, and stained for TRAP at 37oC for 1 hour as described above. Sections were then counterstained with methyl-green for 15 seconds, coverslipped using Permount mounting media (Electron Microscopy Sciences) and allowed to rest for 24 hours before imaging.
Blixt N., Norton A., Zhang A., Aparicio C., Prasad H., Gopalakrishnan R., Jensen E.D, & Mansky K.C. (2020). Loss of Myocyte Enhancer Factor 2 Expression in Osteoclasts Leads to Opposing Skeletal Phenotypes. Bone, 138, 115466.
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2

Sirius Red/Fast Green Staining of Myocardium

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Mid-papillary level transverse myocardial sections were deparaffinized and rehydrated as above. Sirius red/fast green stain was prepared using 0.1% (w/v) Direct Red 80 (Cat. 365,548–5G, Sigma-Aldrich) and 0.1% Fast Green FCF (w/v) (Cat. F7252, Sigma-Aldrich) in picric acid (P6744-1GA, Sigma-Aldrich). Myocardial sections were incubated in sirius red/fast green solution for 30 min at room temperature, rinsed in deionized water, dehydrated with 100% ethanol, rinsed in xylene, and mounted under glass coverslips using Permount Mounting Media (Cat.17986-05, Electron Microscopy Sciences). Brightfield images were digitally acquired using a Keyence BZ-X810 microscope using both 20 × and 4 × objectives.
Sadri G., Fischer A.G., Brittian K.R., Elliott E., Nystoriak M.A., Uchida S., Wysoczynski M., Leask A., Jones S.P, & Moore JB I.V. (2022). Collagen type XIX regulates cardiac extracellular matrix structure and ventricular function. Matrix biology : journal of the International Society for Matrix Biology, 109, 49-69.
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3

Histological Staining and IHC Analysis

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5 μm FFPE sections were deparaffinized using standard protocol
and stained with Harleco® Gill’s Modified Hematoxylin (EMD
Millipore) and eosin (Sigma-Aldrich). Slides remained in xylene until mounted
with Permount Mounting Media (Electron Microscopy Sciences). For IHC staining, 5
μm FFPE sections were subjected to antigen retrieval in boiling 10 mM
sodium citrate (pH 6.0) buffer for 10 minutes. After blocking endogenous
peroxidase with 3% H2O2 for 15 minutes, slides
were washed 3 times in 1× PBS prior to blocking for 60 minutes at room
temperature (Protein Block, Serum-Free; Dako), followed with primary antibody
(STAT1, 1:1000, #14994S, Cell Signaling Technologies; α-SMA,
1:500, #ab124964, Abcam; 1:500. Cytokeratin-8, #ab53280, Abcam)
and incubated overnight at 4 °C. After washing with PBS, the sections
were incubated with secondary antibody for another 60 minutes (Polink-2 Plus HRP
Rabbit with DAB Kit, GBI Labs). Each slide was counterstained with
Gill’s hematoxylin. IHC quantifications were carried out using Image J
(NIH).
Zellmer V.R., Schnepp P.M., Fracci S.L., Tan X., Howe E.N, & Zhang S. (2017). Tumor-induced Stromal STAT1 Accelerates Breast Cancer via Deregulating Tissue Homeostasis. Molecular cancer research : MCR, 15(5), 585-597.
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4

TRAP Staining of Decalcified Bone

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Decalcified bone sections were deparaffinized in xylene, rehydrated through an ethanol gradient, and stained for TRAP at 37 °C for 1 h, as described above. Sections were then counterstained with methyl green for 15 s, cover slipped using Permount mounting media (Electron Microscopy Sciences, Hatfield, PA USA), and allowed to rest for 24 h before imaging.
Maisuria R., Norton A., Shao C., Bradley E.W, & Mansky K. (2023). Conditional Loss of MEF2C Expression in Osteoclasts Leads to a Sex-Specific Osteopenic Phenotype. International Journal of Molecular Sciences, 24(16), 12686.
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5

Histochemical Analysis of Osteoclasts

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Decalcified bone sections were de-paraffinized in xylene, rehydrated through an ethanol gradient, and stained for TRAP at 37 °C for 1 h as described above. Sections were then counterstained with methyl-green for 15 s, cover slipped was placed using Permount mounting media (Electron Microscopy Sciences) and allowed to rest for 24 h before imaging.
Norton A., Thieu K., Baumann C.W., Lowe D.A, & Mansky K.C. (2022). Estrogen regulation of myokines that enhance osteoclast differentiation and activity. Scientific Reports, 12, 15900.
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6

Sirius Red/Fast Green Staining of Myocardium

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mid-papillary level transverse myocardial sections were deparaffinized and rehydrated as above. Sirius red/fast green stain was prepared using 0.1% (w/v) Direct Red 80 (Cat. 365,548–5G, Sigma-Aldrich) and 0.1% Fast Green FCF (w/v) (Cat. F7252, Sigma-Aldrich) in picric acid (P6744-1GA, Sigma-Aldrich). Myocardial sections were incubated in sirius red/fast green solution for 30 min at room temperature, rinsed in deionized water, dehydrated with 100% ethanol, rinsed in xylene, and mounted under glass coverslips using Permount Mounting Media (Cat.17986-05, Electron Microscopy Sciences). Brightfield images were digitally acquired using a Keyence BZ-X810 microscope using both 20 × and 4 × objectives.
Sadri G., Fischer A.G., Brittian K.R., Elliott E., Nystoriak M.A., Uchida S., Wysoczynski M., Leask A., Jones S.P, & Moore JB I.V. (2022). Collagen type XIX regulates cardiac extracellular matrix structure and ventricular function. Matrix biology : journal of the International Society for Matrix Biology, 109, 49-69.
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