Avance dpx 200 nmr spectrometer

Rifampicin was purchased from MilliporeSigma (St. Louis, MO). Except for the glycolipids Man-DPPE and G7-DPPE, which were synthesized as shown below, all other lipids used in this study were purchased from Avanti Polar Lipids (Alabaster, Alabama). Con A was purchased from Vector Laboratories. Other reagents were purchased from either MilliporeSigma or Fisher Scientific (Hampton, NH) and were used without further purification unless otherwise noted. The experimental details for the syntheses of compounds 14–17 can be found in the Supporting Information. Polycarbonate membranes (0.1 μm, 19 mm) were purchased from Avanti Polar Lipid. ¹H NMR and ¹³C NMR spectra were recorded on a Bruker Avance Spectrospin-500 spectrometer or a Bruker Avance DPX 200 NMR spectrometer. ³¹P NMR spectra were recorded on a Bruker Avance DPX 200 NMR spectrometer using 85% H₃PO₄ (0.00 ppm) as the external reference. HRMS spectra were collected in the electrospray ionization (ESI) mode on a Bruker MicrOTOF II mass spectrometer at the University of Massachusetts Amherst. UV–vis spectra were collected on a Perkin Elmer LAMBDA 35 UV–vis spectrophotometer. Dynamic light scattering (DLS) measurements were carried out on a Nano Series Zen 40 03 Zetasizer (Malvern Instruments Ltd, Malvern, UK).