Mir 30a mimics

The miR-30a mimics and negative control (NC) were chemically synthesized by GenePharma Co., Ltd. (Shanghai, China). The sequences of miR-30a mimics and miR-NC were 5′-UGUAAACAUCCUCGACUGGAAG-3′ and 5′-UUCUCCGAACGUGUCACGUTT-3′, respectively. The aging cells were pre-transfected with miR-30a mimics or miR-NC with a final concentration of 50 nM for 48 h, prior to treatment with rapamycin for 12 h. Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) was used to transfect oligonucleotides and constructs into cell lines, according to the manufacturer's instructions. The infection efficiency was confirmed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) or western blotting 48 h post-transfection.

miR-30a mimics (20 nmol/l) and negative control (NC) miRNA (20 nmol/l) were acquired from Shanghai GenePharma Co., Ltd. (Shanghai, China). The miR-30a mimics sequence was 5′-UGUAAACAUCCUCGACUGGAAG-3′. The NC miRNA sequence was 5′-ACAUUUGUAGGAGCUGACCGGC-3′. Lipofectamine 2000^® (Invitrogen; Thermo Fisher Scientific, Inc.) was subsequently utilized to perform transient transfection following incubation at 37°C for 6 h. Transfected cells were collected and purified after 48 h incubation.