Matrine

Manufactured by Selleck Chemicals

Sourced in United States

Matrine is a natural compound derived from the roots of the Sophora plant. It is a colorless, crystalline solid with a molecular formula of C₁₅H₂₄N₂O. Matrine has been studied for its potential biological activities, but its core function is not being interpreted or extrapolated upon in this description.

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Lab products found in correlation

Cck 8 solution, by Dojindo Laboratories (1 mentions) Dmxaa, by Selleck Chemicals (1 mentions) Cycloheximide (chx), by Selleck Chemicals (1 mentions) Anti c myc 10828 1 ap, by Proteintech (1 mentions) Cck 8, by Dojindo Laboratories (1 mentions) Anti β actin, by Proteintech (1 mentions) Actinomycin d, by Selleck Chemicals (1 mentions) Mg132, by Selleck Chemicals (1 mentions)

3 protocols using matrine

Matrine's Short-term Effects on Cell Metabolism

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The short-term effects of matrine (#S2322, Selleck, USA) on cell metabolic activity were assessed using the CCK-8 assay. Cells were seeded at a density of 5000 cells/well in a 96-well plate and treated with matrine at the indicated concentrations (0, 0.2, 0.4, 1, 2, 4, and 6 mM) for 24, 48 or 72 h. CCK-8 solution (#KQ749, DOJINDO, Japan) was added (10 μl/well), and plates were incubated for 4 h at 37 °C. The plates were read on a microplate system (Gen5 GHS 2.0, USA) at 540 nm. Assays were performed in triplicate in 3 independent experiments.

Liu N., Yang C., Yang L., Li T., Gong M., Wang H., Zhang J., Zhao H., Zou L, & He X. (2022). Matrine induces autophagy in human neuroblastoma cells via blocking the AKT-mTOR pathway. Medical Oncology (Northwood, London, England), 39(11), 167.

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DMXAA Modulates PD-L1 and Apoptosis

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C1498FFDsR cells were incubated in Dulbecco modified Eagle complete media (DMEM-c) at 35 degrees. Cells were exposed to increasing concentrations of DMXAA (Selleck Chemicals) ranging from 1–1000 μg/ml to assess PD-L1 expression and apoptosis. Murine interferon gamma (EMD Millipore) served as a positive control for PD-L1 expression, and matrine (Selleck Chemicals) served as a positive control for apoptosis.

Vroom J.M., De Grauw K.J., Gerritsen H.C., Bradshaw D.J., Marsh P.D., Watson G.K., Birmingham J.J, & Allison C. (1999). Depth Penetration and Detection of pH Gradients in Biofilms by Two-Photon Excitation Microscopy. Applied and Environmental Microbiology, 65(8), 3502-3511.

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Matrine Modulation of Cell Signaling

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Matrine (S2322, Selleck, USA) was dissolved in ddH₂O with a final concentration of 40 mg/ml and stored at +4°C. ddH₂O was used as vehicle control for Matrine. Other reagents and antibodies used included: Cycloheximide (CHX, S7418, Selleck, USA), MG132 (S2619, Selleck, USA), Actinomycin D (ACTD, S8964, Selleck, USA), cell counting kit-8 (CCK-8, CK04, Dojindo, Japan), Anti-c-Myc (10828-1-AP, Proteintech group, USA) and Anti-β-actin (23660-1-AP, Proteintech group, USA). DMSO as control for CHX, MG132, and ACTD.

Zhong W.J., Ma L., Yang F., Cao J., Tan J, & Li B. (2022). Matrine, a potential c-Myc inhibitor, suppresses ribosome biogenesis and nucleotide metabolism in myeloid leukemia. Frontiers in Pharmacology, 13, 1027441.

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