Six-week-old female BALB/c (Koatech, Pyung Taek, South Korea) and C57BL/6 (Orient Bio, Sungnam, South Korea) mice were purchased and housed in the Animal Research Facility, International Vaccine Institute (IVI) under standard laboratory conditions. Human dipeptidyl protease 4 (hDPP4) knock-in mice were kindly provided by Paul McCray, University of Iowa, and bred at the IVI. Animal protocols were approved by the Institutional Animal Care and Use Committees of the IVI (2016-004 and 2017-004).
Balb c
Manufactured by Koatech
Sourced in Cameroon
BALB/c is a mouse strain commonly used in laboratory research. It is a well-established model for various studies, including immunology, cancer research, and infectious disease research.
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5 protocols using balb c
1
Transgenic Mice for SARS-CoV-2 Research
2
Maintenance of Toxoplasma gondii Strains in Mouse Models
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T. gondii GT1 strains were maintained in female BALB/c obtained from KOA Tech (Pyeogtaek, South Korea) as described previously (5 (link), 6 (link), 12 (link), 13 (link)). T. gondii GT1 and ME49 strains were maintained by serial intraperitoneal passage (GT1) or oral passage (ME49) in BALB/c mice as described (5 (link)). Spodoptera frugiperda Sf9 cells used for production of recombinant baculovirus (rBV) and virus-like particles were maintained in serum-free SF900 II medium (Invitrogen, Carlsbad, CA, USA) in spinner flasks at 27°C and 130–140 rpm. T. gondii (ME49)–infected sera from mice were used. Horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin IgG was purchased from Southern Biotech (Birmingham, AL, USA). Monoclonal mouse anti-M1 antibody was purchased from Abcam (Cambridge, UK).
3
Mouse Tumor Induction and Measurement
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Six weeks old female mice including BALB/c and C57BL/6 were purchased from KOATECH (Pyeongtaek, Korea). The mice were maintained under specific pathogen-free conditions in the experimental facilities at Kangwon National University (Chuncheon, Korea). All the animal experiments were performed according to the approved guidelines of the Institutional Animal Care and Use committee of Kangwon National University (KW-201007-1). To establish a mouse tumor model, seven weeks old mice were challenged with 2×106 MO5 cells or Her-2/CT26 cells subcutaneously. Tumor length, height, and width were measured using calipers and the tumor volume was calculated as 1/6π×length (mm)×height (mm)×width (mm).
4
Comparative Mouse Model Methodology
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BALB/c and C57BL/6 mice (6–8 wk old, female) were purchased from Koatech (Pyeongtaek, Korea). All mice were maintained in the Seoul National University Hospital Biomedical Research Institute animal facility under specific pathogen-free conditions. All experiments were approved by the Seoul National University Hospital Institutional Animal Care and Use Committee (approval No. 21-0278).
5
Ovalbumin-Induced Allergic Asthma Model
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The experimental mice (6-week-old female BALB/c) were purchased from Koatech Co., Ltd. (Pyeongtaek, Korea). After 1 week of acclimatization, the experimental groups (each, n = 6) were established as follows: (i) normal control (NC); (ii) ovalbumin (OVA), ovalbumin + alum sensitization/ovalbumin inhalation; (iii) dexamethasone (DEX), ovalbumin +1 mg/kg DEX-treatment; (iv) aromadendrin (ARO) 5, ovalbumin +5 mg/kg ARO-treatment; and (v) ARO 10, ovalbumin +10 mg/kg ARO-treatment groups.
The OVA-induced AA was established in the mice as per a previous protocol [5 ]. Briefly, a mixture of OVA and alum was intraperitoneally injected into mice for 2 times at day 0 and day 7, respectively. Mice were exposed to 1 % OVA aerosols from days 11–13 for 1 h/day. ARO (5 or 10 mg/kg) or DEX (1 mg/kg) was orally administered to mice through days 9–13. ARO and DEX were dissolved in 1 % dimethyl sulfoxide and 1 % Tween-20 in PBS.
The OVA-induced AA was established in the mice as per a previous protocol [5 ]. Briefly, a mixture of OVA and alum was intraperitoneally injected into mice for 2 times at day 0 and day 7, respectively. Mice were exposed to 1 % OVA aerosols from days 11–13 for 1 h/day. ARO (5 or 10 mg/kg) or DEX (1 mg/kg) was orally administered to mice through days 9–13. ARO and DEX were dissolved in 1 % dimethyl sulfoxide and 1 % Tween-20 in PBS.
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