Vector x3 multilabel plate reader

Manufactured by PerkinElmer

Sourced in United States

The Vector X3 multilabel plate reader is a versatile instrument designed for various assay types. It can measure absorbance, fluorescence, and luminescence in microplates. The instrument provides reliable and accurate data for a wide range of applications.

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Lab products found in correlation

Celltiter glo assay, by Promega (3 mentions) Caspase glo 3 7 assay, by Promega (2 mentions) Ts2r fl inverted microscope, by Nikon (1 mentions) Dual luciferase reporter assay system, by Promega (1 mentions)

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Multilabel plate reader (45 citations)

4 protocols using vector x3 multilabel plate reader

Caspase-3/7 and Cell Viability Assays

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The Caspase-Glo-3/7 assay (Promega) was used to quantify the activation of executor caspases including caspase-3 and caspase-7. Cells were lysed with the Caspase-Glo-3/7 reagent at the designated time points and were incubated at room temperature for 20 min, followed by measuring the luminescence signal with the Vector X3 multilabel plate reader (PerkinElmer). Cell viability was quantified with the CellTiter-Glo assay (Promega). Cells were lysed together with culture supernatant at a 1:1 ratio (volume) with the CellTiter-Glo reagent and were incubated at room temperature for 10 min, followed by measuring the luminescence signal with the Vector X3 multilabel plate reader (PerkinElmer).

Chu H., Shuai H., Hou Y., Zhang X., Wen L., Huang X., Hu B., Yang D., Wang Y., Yoon C., Wong B.H., Li C., Zhao X., Poon V.K., Cai J.P., Wong K.K., Yeung M.L., Zhou J., Au-Yeung R.K., Yuan S., Jin D.Y., Kok K.H., Perlman S., Chan J.F, & Yuen K.Y. (2021). Targeting highly pathogenic coronavirus-induced apoptosis reduces viral pathogenesis and disease severity. Science Advances, 7(25), eabf8577.

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Quantifying SARS-CoV-2 and SARS-CoV Cytotoxicity

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To ascertain cell damage on SARS-CoV-2 and SARS-CoV infection, we quantified cell viability with the CellTiterGlo assay (Promega, Madison, WI, USA). We lysed cells together with culture supernatant at a ratio of 1:1 (volume) with the CellTiter-Glo reagent and incubated this mixture at room temperature for 10 min, then we measured the luminescence signal with the Vector X3 multilabel plate reader (Perkin Elmer). We obtained images of cellular cytopathic effect on SARS-CoV-2 and SARS-CoV infection at 72 hpi with a Nikon Ts2R-FL inverted microscope (Nikon, Tokyo, Japan).

Chu H., Chan J.F., Yuen T.T., Shuai H., Yuan S., Wang Y., Hu B., Yip C.C., Tsang J.O., Huang X., Chai Y., Yang D., Hou Y., Chik K.K., Zhang X., Fung A.Y., Tsoi H.W., Cai J.P., Chan W.M., Ip J.D., Chu A.W., Zhou J., Lung D.C., Kok K.H., To K.K., Tsang O.T., Chan K.H, & Yuen K.Y. (2020). Comparative tropism, replication kinetics, and cell damage profiling of SARS-CoV-2 and SARS-CoV with implications for clinical manifestations, transmissibility, and laboratory studies of COVID-19: an observational study. The Lancet. Microbe, 1(1), e14-e23.

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Measuring Cell Viability and Apoptosis in Virus-Infected Cells

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Cell viability and Caspase-3/7 activity of mock- or virus-infected MDMs and moDCs were determined using CellTiterGlo assays (Promega, Madison, USA) and CaspaseGlo 3/7 assays (Promega, Madison, USA), respectively [39 (link)]. Briefly, the cells were infected with ZIKV^PR or ZIKV^U (MOI=0.1) for 2 h at 37°C. The cells were lysed together with culture supernatants at a 1:1 ratio with the CellTiterGlo reagent or CaspaseGlo 3/7 reagent at the indicated days post infection and placed on an orbital shaker for 10 min to induce cells lysis. The plates were read by measuring the luminescence signal with the VectorX3 multi-label plate reader (PerkinElmer, Waltham, MA, USA) as we previously described [40 ].

Yang D., Chu H., Lu G., Shuai H., Wang Y., Hou Y., Zhang X., Huang X., Hu B., Chai Y., Yuen T.T., Zhao X., Lee A.C., Ye Z., Li C., Chik K.K., Zhang A.J., Zhou J., Yuan S, & Chan J.F. (2021). STAT2-dependent restriction of Zika virus by human macrophages but not dendritic cells. Emerging Microbes & Infections, 10(1), 1024-1037.

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Dual-Luciferase Assay for MERS-CoV

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Dual-luciferase reporter assay was performed with the Dual-Luciferase Reporter Assay System (Promega). Briefly, Huh7 cells were first cotransfected with the reporter plasmid pCHOP-Luc and the internal control plasmid pRL-SV40, followed by MERS-CoV infection at 1 multiplicity of infection (MOI). At 24 hpi, the infected cell lysates were harvested, and relative luciferase activity was determined by normalizing the relative luciferase unit readout of the firefly luciferase to that of the Renilla luciferase activity measured by the Vector X3 multilabel plate reader (PerkinElmer). Tunicamycin (1 μg/ml)–treated cells were used as a positive control.

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