Rpmi 1640 cell culture media

Absolute Mag amine MNPs (dextran‐coated, 50 nm) were purchased from CD Bioparticles (NY, USA). Bovine serum albumin, glutaraldehyde solution (25%), ConA, DAPI, Tween 20, PepStrep, and Corning Costar TC‐treated multiple‐well plates were purchased from Sigma‐Aldrich Inc. (Saint Louis, MO, USA). RPMI 1640 cell culture media, 96‐well enzyme‐linked immunosorbent assay (ELISA) plates, 1× RBC lysis buffer, CD3ε monoclonal antibody (145‐2C11), NovaFlour700 tagged CD3ε monoclonal antibody, rabbit antihamster IgG (H+L), secondary antibody FITC labeled, and Pierce BCA Protein Assay Kit were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Cell counting kit‐8 (CCK‐8) assay was purchased from Dojindo Laboratories Co. Ltd., Kumamoto, Japan. TEM copper grid support films, carbon 300 mesh, were purchased from Ted Pella, Inc. (Redding, CA, USA). The antifade mounting medium was purchased from Abcam (Cambridge, UK). Mouse IFN‐γ and IL‐6 ELISA kits were purchased from BD Biosciences (San Jose, CA, USA).

For these experiments, 20, 70 - dichlorofluorescein diacetate (DCFH-DA), vitamin C, and linoleic acid (LA) were purchased from Sigma (Shanghai, China). Fetal bovine serum (FBS) and RPMI-1640 cell culture media were purchased from Thermo Fisher (Shanghai, China). The primary antibodies against Poly (ADP-ribose) polymerase (PARP), procaspase-8, cleaved caspase-3,NFκB, p65, CDK1, Cyclin D1, CDK2, IκB, p-IκB, Fas, Bcl-2, Bax, Cyto-c, and ND1 antibodies were provided by Cell Signaling Technology (Shanghai, China). α-tublin, lamin B, β-actin, GAPDH, HO-1, NQO-1, iNOS, COX-2, p53, Nrf2, keap-1 were from Santa Cruz Biotechnology (Dallas, Texas, USA). Cell cycle kit and Annexin V/PI cell apoptosis kit were from Wanleibio Company (Shenyang, Liaoning, China). All other chemicals were of analytical grade and were produced in China.

RPMI 1640 cell culture media, fetal bovine serum (FBS) and Penicillin-Streptomycin antibiotic were obtained from Gibco^® Life Technologies (Waltham, MA, USA). Ethanol, chloroform and isopropanol were purchased from Merck (Kenilworth, NJ, USA). RNX-Plus solution was obtained from SinaClon BioScience (Tehran, Iran). Reverse transcriptase, RNase inhibitor, dNTPs, and random hexamer primers were provided from Thermo FisherScientific (Waltham, MA, USA). TPP^® tissue culture flasks (filter cap), βBA (80342-5MG) and all other chemicals were purchased from Sigma (St Louis, MO, USA).

DMEM/F12, DMEM high glucose, and RPMI 1640 cell culture media were purchased from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was obtained from Alfa Aesar (Waltham, MA, USA). Luteolin and lucidin were purchased from PhytoLab (Vestenbergsgreuth, Germany), and alizarin and taxifolin were acquired from Sigma Aldrich (St. Louis, MO, USA). Propidium iodide solution and Hoechst 33,342 were also bought from Sigma Aldrich (St. Louis, MO, USA). A Caspase 3/7 Glo Kit was obtained from Promega (Madison, WI, USA). All the other chemicals and solvents obtained commercially were of analytical grade. All solutions were freshly prepared by using ultra-pure grade water purified with a Milli-Q system from Millipore (Billerica, MA, USA).

Short-term tumor cell cultures were established as previously described in [19 (link)]. Briefly, fresh tumor tissue was obtained intra-operatively and transferred to the cell culture facility. Tissue specimens were mechanically minced and incubated in an enzyme cocktail consisting of 40 U/mL collagenase (Sigma-Aldrich, St. Louis, MO, USA), 125 U/mL DNAse (Sigma-Aldrich), and 100 U/mL hyaluronidase (Sigma-Aldrich) at 37 °C for 45 min. Cells were passed through stainless steel sieves and suspended in RPMI 1640 cell culture media supplemented with 5% fetal bovine serum, 100 U/mL of penicillin, 100 U/mL of streptomycin, and 1% l-glutamine (all from Gibco, Carlsbad, CA, USA). All experimental procedures were approved by the Ethics Committee of the University of Heidelberg School of Medicine (S-453/2010). Written informed consent was obtained from all patients.