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Protein deglycosylation kit

Manufactured by Merck Group
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The Protein Deglycosylation kit is a laboratory tool designed to remove glycosylation from proteins. It contains reagents and enzymes that facilitate the deglycosylation process, which can be useful for various research and analytical applications.

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Lab products found in correlation

Bovine serum albumin (bsa), by Merck Group (1 mentions) Human fibronectin, by Merck Group (1 mentions) Vicia villosa agglutinin vva, by Vector Laboratories (1 mentions) Peanut agglutinin pna, by Vector Laboratories (1 mentions) Human collagen 4, by Merck Group (1 mentions) Murine laminin, by Merck Group (1 mentions) Antibodies against c1galt1, by Santa Cruz Biotechnology (1 mentions) Huts 21, by BD (1 mentions)

Spelling variants of this product

Enzymatic Protein Deglycosylation Kit (11 citations) EDEGLY enzymatic protein deglycosylation kit (2 citations) Native protein deglycosylation kit (2 citations)

3 protocols using protein deglycosylation kit

1

Deglycosylation of Recombinant Pneumolysin

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PLY was deglycosylated under native conditions using the Protein Deglycosylation kit (Sigma) following the instructions of the kit. Briefly, 10 μg of recombinant PLY was incubated with 1 μl each of Peptide:N-glycosidase F, O-Glycosidase, Sialidase A, ß-(1-4)-Galactosidase and ß-N-acetylglucosaminidase in 50 μl reaction buffer for 3 days at 37°C. The extent of deglycosylation was assessed by mobility shifts on SDS-PAGE gels.
Subramanian K., Neill D.R., Malak H., Spelmink L., Khandaker S., Marchiori G.D., Dearing E., Kirby A., Yang M., Achour A., Nilvebrant J., Nygren P.Å., Plant L., Kadioglu A, & Henriques-Normark B. (2018). Pneumolysin binds to the Mannose-Receptor C type 1 (MRC-1) leading to anti-inflammatory responses and enhanced pneumococcal survival. Nature microbiology, 4(1), 62-70.
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2

Integrin β1 Activation and Glycosylation

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Antibodies against total integrin β1 and activated integrin β1 (HUTS-21) were purchased from BD Biosciences (Lincoln Park, NJ). Integrin β1 blocking antibody (P4C10) was purchased from Millipore (Billerica, MA). Antibodies against C1GALT1, GAPDH, and focal adhesion kinase (FAK) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Antibody against phospho (p)-FAK was purchased from Cell Signaling Technology Inc. (Beverly, MA). Antibody against actin was purchased from GeneTex Inc. (Irvine, CA). Vicia villosa agglutinin (VVA) and peanut agglutinin (PNA) lectins were purchased from Vector Laboratories (Burlingame, CA). Human collagen IV, human fibronectin, murine laminin, bovine serum albumin (BSA), and protein de-glycosylation kit were purchased from Sigma (St Louis, MO).
Liu C.H., Hu R.H., Huang M.J., Lai I.R., Chen C.H., Lai H.S., Wu Y.M, & Huang M.C. (2014). C1GALT1 Promotes Invasive Phenotypes of Hepatocellular Carcinoma Cells by Modulating Integrin β1 Glycosylation and Activity. PLoS ONE, 9(8), e94995.
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3

Deglycosylation of Recombinant Pneumolysin

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PLY was deglycosylated under native conditions using the Protein Deglycosylation kit (Sigma) following the instructions of the kit. Briefly, 10 μg of recombinant PLY was incubated with 1 μl each of Peptide:N-glycosidase F, O-Glycosidase, Sialidase A, ß-(1-4)-Galactosidase and ß-N-acetylglucosaminidase in 50 μl reaction buffer for 3 days at 37°C. The extent of deglycosylation was assessed by mobility shifts on SDS-PAGE gels.
Subramanian K., Neill D.R., Malak H., Spelmink L., Khandaker S., Marchiori G.D., Dearing E., Kirby A., Yang M., Achour A., Nilvebrant J., Nygren P.Å., Plant L., Kadioglu A, & Henriques-Normark B. (2018). Pneumolysin binds to the Mannose-Receptor C type 1 (MRC-1) leading to anti-inflammatory responses and enhanced pneumococcal survival. Nature microbiology, 4(1), 62-70.
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