HUVEC, seeded in 96-well microplates, were treated with RiduROS (0.1, 1, and 10 μg/mL) and the effects on cell viability was monitored at 24 and 48 h after treatment. The viability of HUVEC was assayed by colorimetric assay using WST-1 (Biovision, San Francisco, USA) which was based on cleavage in viable cells of the water-soluble tetrazolium salt WST-1 [2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disul-fonyl)-2H-tetrazolium, monosodium salt] to a formazan dye by mitochondrial dehydrogenase. Briefly, cells cultured in 96-well plates were treated with different concentrations of RiduROS or a single antioxidant component for 24–48 h. Then, 10 μL WST-1 reagent was added to each well and HUVEC were incubated for 4 h at 37°C. The formazan dye produced was quantified by measuring the absorbance of the dye solution at 450 nm with a microplate reader.
Wst 1
Manufactured by Abcam
Sourced in United States, United Kingdom
WST-1 is a colorimetric assay kit used for the quantitative measurement of cell proliferation and cell viability. It utilizes a tetrazolium salt which is cleaved to a colored formazan dye by mitochondrial dehydrogenases in viable cells. The amount of formazan produced is directly proportional to the number of living cells in the sample.
Automatically generated - may contain errors
Lab products found in correlation
Wst 1, by BMG Labtech (2 mentions)
Fluostar omega, by BMG Labtech (2 mentions)
Infinite m200 pro, by Tecan (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Chitosan, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Wst 1 reagent, by Abcam (1 mentions)
Prism software, by GraphPad (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Wst 1, by Merck Group (1 mentions)
D glucose anhydrous, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Bromodeoxyuridine (brdu), by Roche (1 mentions)
Mtt reagent, by Merck Group (1 mentions)
Xcelligence rtca dp system, by Agilent Technologies (1 mentions)
E plate view 16, by Agilent Technologies (1 mentions)
18 protocols using wst 1
1
HUVEC Viability Assay with RiduROS
2
Assessing HUVEC Viability on PLGA Scaffold
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To examine HUVECs viability during culturing on our PLGA scaffold, a ready-to-use cell proliferation reagent WST-1 (Cat: K304, BioVision, Milpitas, CA, USA) was used. PLGA 50/50 powder was dissolved in acetone in a 1:7 w/w ratio and gently separated onto the flat PDMS mold to make flat PLGA membranes. The flat PLGA membranes were then cut into small circles which fitted the area of the 96 wells and rinsed with 70% alcohol followed by exposed under UV light overnight. Those circular PLGA membranes were then stuck on the bottom of a 96-well dish and coated with 0.5% gelatin. 1500 HUVECs were seeded in each well and WST-1 was used for measuring the increment of cell numbers after culturing for three and seven days.
3
Cisplatin Cytotoxicity Assay using WST-1
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Drug sensitivity was measured by a WST-1 assay [37 (link)]. Cells were seeded at different cell numbers in a 96-well plate 18 h prior to drug challenge. Cells were pulse-treated with various concentrations of cisplatin for 4 h. The control cells were treated with the solvent of the drug. Total survival of the cells was determined 72 h following drug challenge, and percent survival was estimated by dividing optical absorbance resulted from each experiment group with that of the control group. Each experiment was done in triplicates, and the optical absorbance was measured by coloration of reacted substrate, WST-1 (BioVision, Mountain View, CA), which was catalyzed by mitochondrial dehydrogenases.
4
Evaluating anti-c-Src antibody effects on prostate cancer cell lines
5
Cell Viability Assay Protocol
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Cells were seeded at 1000, 2500, and 5000 cells/well, 18 hours prior to drug challenge. The cells were then treated continuously with various drug concentrations for 72 hours. The control group was only treated with the drug diluent, PBS or dimethyl sulfoxide. Culture media were replaced with PBS before the addition of 10 μL of WST-1 (BioVision, Mountain View, CA, USA) and incubation was continued for 4 hours. The percentage of surviving cells in the experiment group was quantified and expressed relative to the control group. All procedures were performed in triplicate [8 (link)].
6
Cell Viability Assay via WST-1
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ULM and/or MM cells were cultured in 96-well plates in complete DMEM-F12 1:1 medium until 80 to 90% confluence. At the end of treatments, WST-1 (BioVision) was added to each well (1:10 dilution) for 2 hours at 37°C, and absorbance was read at 420 nm with reference wavelength set at 650 nm. Data were analyzed following the manufacturer’s manual.
7
Cell Viability Assay using WST-1
8
Drug Sensitivity Assay in Cell Cultures
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Drug-sensitivity was measured by a WST-1 assay [40] . Cells were seeded at 100, 1,000, and 5,000 cells/96-well plates 18 hr prior to drug challenge. Cells were pulse-treated with 4 μM of daunorubicin for 2 hr. The negative control cells were treated with the solvent for the drug. Total survival of the cells was determined at 72 hr after the drug challenge, and percent survival was estimated by dividing the optical absorbance resulting from each experimental group with that of the control group. Each experiment was done in triplicate, and the optical absorbance was measured by the coloration of the reacted substrate, WST-1 (BioVision, Mountain View, CA), which was catalyzed by mitochondrial dehydrogenases.
. Statistical analysis.
Overall survival (OS) was de ned as the time from the date of diagnosis to the date of death. Survival curves were plotted using the Kaplan-Meier estimator [41] and the statistical difference in survival between the different groups was compared by a log-rank test [42] . Statistical tests were two-sided, and p < 0.05 was considered signi cant. The t-test was utilized to compare the numerical differences in clinical parameters. Differences in patients' performance status, tumor location, and surgical resection status were assessed by c-square or Fisher's exact test. Analyses of the data were performed using SPSS 10.3 software (Chicago, IL).
. Statistical analysis.
Overall survival (OS) was de ned as the time from the date of diagnosis to the date of death. Survival curves were plotted using the Kaplan-Meier estimator [41] and the statistical difference in survival between the different groups was compared by a log-rank test [42] . Statistical tests were two-sided, and p < 0.05 was considered signi cant. The t-test was utilized to compare the numerical differences in clinical parameters. Differences in patients' performance status, tumor location, and surgical resection status were assessed by c-square or Fisher's exact test. Analyses of the data were performed using SPSS 10.3 software (Chicago, IL).
9
Drug Sensitivity Assay in Cell Cultures
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Drug-sensitivity was measured by a WST-1 assay [40] . Cells were seeded at 100, 1,000, and 5,000 cells/96-well plates 18 hr prior to drug challenge. Cells were pulse-treated with 4 μM of daunorubicin for 2 hr. The negative control cells were treated with the solvent for the drug. Total survival of the cells was determined at 72 hr after the drug challenge, and percent survival was estimated by dividing the optical absorbance resulting from each experimental group with that of the control group. Each experiment was done in triplicate, and the optical absorbance was measured by the coloration of the reacted substrate, WST-1 (BioVision, Mountain View, CA), which was catalyzed by mitochondrial dehydrogenases.
. Statistical analysis.
Overall survival (OS) was de ned as the time from the date of diagnosis to the date of death. Survival curves were plotted using the Kaplan-Meier estimator [41] and the statistical difference in survival between the different groups was compared by a log-rank test [42] . Statistical tests were two-sided, and p < 0.05 was considered signi cant. The t-test was utilized to compare the numerical differences in clinical parameters. Differences in patients' performance status, tumor location, and surgical resection status were assessed by c-square or Fisher's exact test. Analyses of the data were performed using SPSS 10.3 software (Chicago, IL).
. Statistical analysis.
Overall survival (OS) was de ned as the time from the date of diagnosis to the date of death. Survival curves were plotted using the Kaplan-Meier estimator [41] and the statistical difference in survival between the different groups was compared by a log-rank test [42] . Statistical tests were two-sided, and p < 0.05 was considered signi cant. The t-test was utilized to compare the numerical differences in clinical parameters. Differences in patients' performance status, tumor location, and surgical resection status were assessed by c-square or Fisher's exact test. Analyses of the data were performed using SPSS 10.3 software (Chicago, IL).
10
Chitosan-Hypericum Perforatum Oil Cytotoxicity
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Chitosan (low molecular weight, degree of deacetylation 85%) was purchased from Sigma. Hypericum perforatum oil (Alvin,Turkey) was purchased from local pharmacy. Acetic acid and ethanol (Merck, Germany) were used as solvents. WST-1 was purchased from Biovision (USA) for cytotoxicity assay. Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum (FBS), phosphate-buffered saline (PBS), Penicillin-streptomycin antibiotic solution were purchased from Gibco (USA). DAPI (4 , 6-diamidino-2-phenylindole, dihydrochloride) (Cell Signaling, USA) and Alexa Four 488 Phalloidin (Molecular Probes TM ,Thermo Fisher Scientific) stains were used for fluorescence imaging. 37% paraformaldehyde (Merck, Germany) was used for fixation of cells.
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