Ergocalciferol

Manufactured by Merck Group

Sourced in United States, Germany

Ergocalciferol is a form of vitamin D commonly used in laboratory settings. It functions as a precursor to the active form of vitamin D, supporting research and analysis related to vitamin D metabolism and homeostasis.

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Lab products found in correlation

13 protocols using ergocalciferol

E. coli LPS and Human TNF mAb Protocol

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Escherichia coli (0111:B4) LPS was purchased from Sigma-Aldrich (MO, USA). Human TNF mAb was purchased from R&D Systems (Abingdon, U.K.). qPCR was performed by using SYBR^® Premix EX Taq™ II (Takara, Japan). 5′-GMP-Na and ergocalciferol (as vitamin D₂) were purchased from Sigma-Aldrich (MO, USA).

Minato K.I., Ohara A, & Mizuno M. (2017). A Proinflammatory Effect of the β-Glucan from Pleurotus cornucopiae Mushroom on Macrophage Action. Mediators of Inflammation, 2017, 8402405.

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Analytical Standards for Bioanalytical Assays

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Caffeine (99.0%), 3,5-dimethyl-1-hexyn-3-ol (surfynol 61, 98%), docosanoic acid (99%) ergocalciferol (vitamin D2, 98%), β-estradiol (98%), ethyl acetate (99.8%), 5-fluorouracil (99%), lauric acid (99.5%), lin oleic acid (99%), methanol (99.9%), oleic acid (99%), stearic acid (95%), and thymol (98.5%) were all purchased from Sigma Aldrich (St. Louis, MO, USA). Phenol (99%), and stereo 2X-4X microscope (S 15019975) was provided by Fisher Scientific (Pittsburgh, PA, USA). 1.0 mg/mL standard solution of cocaine and 100 μg/mL of cocaine-D3 were acquired from Cerilliant (Round Rock, TX, USA). Di-chloromethane (99.9%) was supplied by Acros Organics (Geel, Belgium). Both single donor human blood and single donor human serum were obtained from Innovative Research (Novi, MI, USA). Bovine blood was provided by LAMPIRE Biological Laboratories (Pipersville, PA, USA). 18.2 MΩ water was used for water solutions (Milli-Q water purification system, Millipore, Billerica, MA, USA). Borosilicate capillaries (ID 1.17 mm) were provided by Sutter Industries (Novato, CA, USA).

Kulyk D.S., Swiner D.J., Sahraeian T, & Badu-Tawiah A.K. (2019). Direct Mass Spectrometry Analysis of Complex Mixtures by Nano-Electrospray with Simultaneous Atmospheric Pressure Chemical Ionization and Electrophoretic Separation Capabilities. Analytical chemistry, 91(18), 11562-11568.

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Cellular Uptake Regulation of Cholesterol and Vitamin D Metabolites

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D₃, ergocalciferol (vitamin D₂), 25(OH)D₃, 1α(OH)D₃, retinyl acetate, 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (phosphatidylcholine), 1-palmitoyl-sn-glycero-3-phosphocholine (lysophosphatidylcholine), monoolein, free cholesterol, oleic acid, sodium taurocholate, and simvastatin were purchased from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Ezetimibe beta-d-glucuronide was purchased from Sequoia Research (Pangbourne, UK). Block lipid transport-1 (BLT1) was purchased from ChemBridge (San Diego, CA, USA). Dulbecco’s modified Eagle’s medium (DMEM) containing 4.5 g/L glucose and trypsin-EDTA (500 mg/L and 200 mg/L, respectively), non-essential amino acids, penicillin/streptomycin and PBS were purchased from Life Technologies (Illkirch, France), and fetal bovine serum (FBS) came from PAA (Vélizy Villacoublay, France). ASBT tagged with the DDK peptide (DYKDDDDK flag peptide) in pCMV plasmid and DDK antibody were purchased from Origen (Austin, TX, USA). All solvents used were HPLC grade (Carlo Erba Réactifs-SdS, Val de Reuil, France).

Desmarchelier C., Margier M., Prévéraud D.P., Nowicki M., Rosilio V., Borel P, & Reboul E. (2017). Comparison of the Micellar Incorporation and the Intestinal Cell Uptake of Cholecalciferol, 25-Hydroxycholecalciferol and 1-α-Hydroxycholecalciferol. Nutrients, 9(10), 1152.

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Comprehensive Extraction and Analysis Protocol

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N-hexane, potassium iodide (KI), sulphuric acid, boric acid, sodium sulfate, diethyl ether, potassium hydroxide, sodium thiosulphate, acetone, acetic acid, chloroform, ethanol, and methanol were procured from Guangdong Chemical Factory (Guangdong, China). Ethylacetate, Wijjs reagent, isopropyl alcohol (lichrosolv), alpha/gamma tocopherol, ergosterol, cholecalciferol, ergocalciferol, campesterol, and sitosterol were procured from Sigma-Aldrich (USA). Others like tetrahydrofuran (THF), acetonitrile, betacarotene, alpha/gamma carotene reference standards were procured from Shandong Yanshuo Chemical Co., Ltd. (Linzi, China). All chemicals/reagents employed in the study were of analytical grade standard.

Omeje K.O., Ezema B.O., Ozioko J.N., Omeje H.C., Ossai E.C., Eze S.O., Okpala C.O, & Korzeniowska M. (2022). Biochemical characterization of Soxhlet-extracted pulp oil of Canarium schweinfurthii Engl. fruit in Nigeria. Scientific Reports, 12, 10291.

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Lipidomic Standards and Reagents

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HPLC-grade methyl tert-butyl ether (MTBE), and ammonium acetate, and Optima-grade acetonitrile, isopropanol, water, methanol, and formic acid were purchased from Fisher Scientific (Hampton, NH, USA). Authentic standards of retinyl palmitate and ergocalciferol were purchased from Sigma Aldrich (St. Louis, MO, USA). (6, 19, 19-D₃, 97%)-ergocalciferol was purchased from Cambridge Isotopes (Andover, MA, USA), and (12, 13, 20-¹³C₃)-retinyl palmitate was purchased from Buchem BV (Apeldoorn, Netherlands). Authentic standards of sphingomyelin (SM) 18:1/16:0, phosphatidylcholine (PC) 18:1/16:0, PC 16:0/20:4, PC 18:2/18:2, and PC 18:1/18:1 were purchased from Cayman Chemical (Ann Arbor, MI, USA). PC 16:0/20:3 and PC 18:0/18:2 were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Cholesterol was purchased from Sigma Aldrich (St. Louis, MO, USA) (see Supporting Information Table S1 for product numbers and full chemical names).

Chatelaine H., Dey P., Mo X., Mah E., Bruno R.S, & Kopec R.E. (2020). Vitamin A and D Absorption in Adults with Metabolic Syndrome vs. Healthy Controls – A Pilot Study Utilizing Targeted and Untargeted LC-MS Lipidomics. Molecular nutrition & food research, 65(2), e2000413.

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Comprehensive Analysis of Fat-Soluble Vitamins and Carotenoids

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tert-Butyl methyl ether (99%, catalog 34875, MTBE, chromatographic grade), methanol (≥99.9%, catalog 646377, MeOH, chromatographic grade) and potassium hydroxide (ACS reagent, catalog 1050210250) were acquired from Merck Millipore (Burlington, MA, USA). Retinyl acetate (catalog 46958), retinyl palmitate (catalog 46959-U), cholecalciferol (catalog C9774), ergocalciferol (catalog 47768), 3-phytylmenadione catalog (95271), α-tocopherol (catalog 47783), δ-tocopherol (catalog 47784), γ-tocopherol (catalog T1782), tocopherols (mixed, W530066), lutein (catalog 071068), β-carotene (catalog C4582), β-cryptoxanthin (≥97%, catalog C6368), zeaxanthin (catalog 14681), all-trans-astaxanthin (catalog 41659), and lycopene (catalog 75051) from Sigma-Aldrich (unless stated otherwise, all standards were of analytical grade, St. Louis, MO, USA). Chloroform (ACS reagent, catalog 366919), ethanol (200 proof, ACS reagent, ≥99.5%, 459844), 2-propanol (HPLC Plus, 650447), pyrogallol (ACS reagent, ≥99%, catalog 16040), and sodium sulfate (anhydrous, granular, free-flowing, Redi-Dri™, ACS reagent, ≥99%, catalog 798592) were also acquired from Sigma-Aldrich.

Cortés-Herrera C., Chacón A., Artavia G, & Granados-Chinchilla F. (2019). Simultaneous LC/MS Analysis of Carotenoids and Fat-Soluble Vitamins in Costa Rican Avocados (Persea americana Mill.). Molecules, 24(24), 4517.

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Vitamin and Sterol Analysis Protocol

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Vitamin standards that include thiamin hydrochloride, riboflavin, nicotinic acid and nicotinamide, calcium-D-pantothenate, pyridoxin-hydrochloride, biotin, folic acid, cyanocobalamin and phylloquinone, were purchased from AccuStandard, New Haven, CT, USA. The following standards and reagents were obtained from Sigma-Aldrich, (Seoul, Korea): pyridoxal-hydrochloride (B₆), all-trans retinol, all-trans tocopherol (E), ergocalciferol (D₂₎, cholecalciferol (D₃), all-trans lutein, butylated hydroxytoluene (BHT), HPLC grade solvent (methanol, ethanol, acetonitrile, and hexane). The HPLC grade MTBT (tert-Butyl methyl ether) was purchased from Thermo Fisher, Korea. Moreover, 7-dehydrocholesterol (DHC) was purchased from Cayman Chemical (MI, USA). Potassium hydroxide, ascorbic acid and sodium chloride were purchased from Deajung. Co. Ltd. (Busan, Korea).

Nguyen M.T., Kim J., Lee H., Won S., Kim Y., Jung J.A., Li D., To X.H., Huynh K.T., Le T.V., Israr B., An H.J, & Kim J. (2020). A Comparison of Vitamin and Lutein Concentrations in Breast Milk from Four Asian Countries. Nutrients, 12(6), 1794.

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Vitamin D Metabolite Quantification

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Cholecalciferol, ergocalciferol, 25-hydroxyergocalciferol, 1α,25-dihydroxyergocalciferol, and ammonium formate were purchased from Sigma-Aldrich GmbH (Steinheim, Germany). The compound 24,25-dihydroxyergocalciferol was purchased from IsoSciences LLC (Ambler, PA, USA). Methanol and acetonitrile were purchased from Carl Roth GmbH & Co. KG (Karlsruhe, Germany). Formic acid was purchased from VWR International GmbH (Darmstadt, Germany) and D(+)-glucuronic acid sodium salt monohydrate was purchased from Merck Chemicals GmbH (Darmstadt, Germany). All of the chemicals were used at the highest quality available. Water was purified before utilization via the Direct-Q 3 UV-R system (Merck Chemicals GmbH, Darmstadt, Germany).

Navarro Suarez L., Thein S., Kallinich C, & Rohn S. (2019). Electrochemical Oxidation as a Tool for Generating Vitamin D Metabolites. Molecules, 24(13), 2369.

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Antioxidant Compounds Characterization

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2,2-Diphenyl-1-picrylhydrazyl radical (DPPH^●), 2,2-Diphenyl-1-picrylhydrazin (DPPH), 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), FeCl₂·4H₂O, FeCl₃·6H₂O, TROLOX, quercetin, rutin, baicalein, morin, 7,8-dihydroxy flavone, (−)-epicatechin, (+)-catechin, l-ascorbic acid, tannic acid, crocin, -caroten, purpurin, silibinin, olivetol, gallic acid, caffeic acid, protocatechuic acid, avenanthramides A, B, and C, flavone, ergocalciferol, lipoic acid, biotin, thiamin, indole-3-carbinol, astaxanthin, uric acid, sodium acetate, ethanol, and acetic acid were purchased from Merck /Sigma/ (St. Louis, MO, USA).

Maliar T., Maliarová M., Blažková M., Kunštek M., Uváčková Ľ., Viskupičová J., Purdešová A, & Beňovič P. (2023). Simultaneously Determined Antioxidant and Pro-Oxidant Activity of Randomly Selected Plant Secondary Metabolites and Plant Extracts. Molecules, 28(19), 6890.

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Antioxidant Compounds Characterization

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