Peripheral blood mononuclear cells (PBMCs) were isolated from blood samples using a Ficoll gradient (Axis Shield, Norway). Unstimulated PBMCs were stained with APC anti-human CD19 antibody (BioLegend cat #302212), APC/Cyanine7 anti-human CD138 antibody (BioLegend cat #356528), PerCP/Cyanine5.5 anti-human CD1d antibody (BioLegend cat #350312), APC anti-human CD25 antibody (BioLegend cat #302610), and PE anti-human CD223 (LAG-3) antibody (BioLegend cat #369306). After surface staining, cells were stained with PE anti-human EBi3 antibody (BioLegend cat #360904) and PE/Cy7 anti-human IL-10 antibody (BioLegend cat #501420), FITC anti-human FOXP3 antibody (BioLegend cat #320106). Corresponding isotype controls were used for each antibody. All cells were analyzed with BD LSR II flow cytometer using DIVA software version 8.0.
Pe anti human cd223 lag 3 antibody
Manufactured by BioLegend
The PE anti-human CD223 (LAG-3) antibody is a fluorochrome-conjugated monoclonal antibody that specifically binds to the CD223 (also known as LAG-3) cell surface receptor on human cells. CD223 is an inhibitory receptor that plays a role in the regulation of T cell activation and function.
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Lab products found in correlation
Apc anti human cd19 antibody, by BioLegend (2 mentions)
Fitc anti human foxp3 antibody, by BioLegend (2 mentions)
Ficoll gradient, by Axis-Shield (2 mentions)
Apc cyanine7 anti human cd138 antibody, by BioLegend (2 mentions)
Percp cyanine5.5 anti human cd1d antibody, by BioLegend (2 mentions)
Apc anti human cd25 antibody, by BioLegend (2 mentions)
Pe anti human ebi3 antibody, by BioLegend (2 mentions)
Pe cy7 anti human il 10 antibody, by BioLegend (2 mentions)
Lsr 2 flow cytometer, by BD (2 mentions)
2 protocols using pe anti human cd223 lag 3 antibody
1
Characterization of Immune Cell Subsets in PBMCs
2
Phenotypic Characterization of PBMCs
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Isolation of peripheral blood mononuclear cells (PBMCs) was performed using a Ficoll gradient (Axis-Shield, Norway). The unstimulated PBMCs were stained with APC anti-human CD19 antibody (BioLegend cat #302212), APC/Cyanine7 anti-human CD138 antibody (BioLegend cat #356528), PerCP/Cyanine5.5 anti-human CD1d antibody (BioLegend cat #350312), APC anti-human CD25 antibody (BioLegend cat #302610), and PE anti-human CD223 (LAG-3) antibody (BioLegend cat #369306). Following surface staining, cells were stained with PE anti-human EBi3 antibody (BioLegend cat #360904), PE/Cy7 anti-human IL-10 antibody (BioLegend cat #501420), and FITC anti-human FOXP3 antibody (BioLegend cat #320106). For each antibody, the corresponding isotype controls were used. All cells were analyzed with BD LSR II flow cytometer using DIVA software version 8.0.
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