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Mouse albumin elisa

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The Mouse Albumin ELISA is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed to measure the concentration of albumin in mouse serum or plasma samples. The assay utilizes antibodies specific to mouse albumin and provides a reliable method for the detection and quantification of this protein.

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Lab products found in correlation

Nanozoomer, by Hamamatsu Photonics (2 mentions) Urea ct fs, by DiaSys (1 mentions) Colorimetric protein assay, by Bio-Rad (1 mentions) Trypan blue solution, by Thermo Fisher Scientific (1 mentions) Human sp a, by BioVendor (1 mentions) Synergy 2 plate reader, by Agilent Technologies (1 mentions) Creatinine assay, by R&D Systems (1 mentions) Freestyle freedom lite glucometer, by Abbott (1 mentions) Pageblue, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Mouse Albumin ELISA Assay kit Mouse Albumin ELISA Quantitation Set Mouse Albumin ELISA kit E99-134 Mouse/rat albumin ELISA kit Mouse urine albumin ELISA kit Anti-mouse albumin ELISA kit Mouse albumin ELISA quantification set Mouse albumin ELISA kit Mouse urinary albumin ELISA kit Mouse Albumin ELISA Quantification Kit

13 protocols using mouse albumin elisa

1

Cytokine Levels in Histone-Exposed Cells

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Supernatant fluids from histone-exposed PMNs or macrophages or cell lines collected at 4 hr, and the levels of the TNF, IL-6 and IL-1β cytokines measured using ELISA kits (R&D Systems, Minneapolis, MN). Mouse albumin ELISA from Bethyl Laboratories (Montgomery, TX) was used to detect the amount of albumin leak into lung according to the manufacturer’s instructions. BALFs were also examined for content of cytokines (TNF, IL-6 and IL-1β) using the ELISA kits.
Fattahi F., Grailer J.J., Lu H., Dick R.S., Parlett M., Zetoune F.S., Nuñez G, & Ward P.A. (2017). Selective Biological Responses of Phagocytes and Lungs to Purified Histones. Journal of Innate Immunity, 9(3), 300-317.
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2

Measurement of Urine and Plasma Biomarkers

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Urine albumin was measured with a mouse albumin ELISA (Bethyl Laboratories Inc. Montgomery, TX). Sodium and potassium excretion were determined by flame photometry. Plasma urea was measured by DiaSys Urea CT FS (DiaSys Diagnostic Systems, Holzheim, Germany).
Oosterhuis N.R., Papazova D.A., Gremmels H., Joles J.A, & Verhaar M.C. (2017). T-cells contribute to hypertension but not to renal injury in mice with subtotal nephrectomy. BMC Nephrology, 18, 153.
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3

Bronchoalveolar Lavage Fluid Analysis

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Bronchoalveolar lavage fluid (BALF) was collected via intratracheal instillation in 2 fractions (after cardiac blood draw). The first fraction (1.0 mL PBS, 0.4mM EDTA) was used for cell counting via hemocytometer (diluted 1:1 with Trypan Blue Solution, 0.4%; Life Technologies, Grand Island, NY). A second fraction (1.0 mL PBS, 0.4mM EDTA) was used for total protein (colorimetric protein assay; Bio-Rad, Hercules, CA) and albumin (mouse albumin ELISA; Bethyl Laboratories, Montgomery, TX).
Conklin D.J., Haberzettl P., Jagatheesan G., Kong M, & Hoyle G.W. (2016). Role of TRPA1 in Acute Cardiopulmonary Toxicity of Inhaled Acrolein. Toxicology and applied pharmacology, 324, 61-72.
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4

Quantification of Lung Surfactant Proteins by ELISA

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The ELISA measurements were performed as per manufacturer’s protocol. Mouse ELISAs. Mouse SP-D (Cat # 028248) and SP-A (Cat # 028239) from USBiological Life Sciences and mouse CC16 (Cat# EKU03200) ELISA was purchased from Biomatik. Mouse albumin ELISA (Cat# E90-134) was from Bethyl Laboratories, Inc. Human SP-A, SP-D and CC16 were also measured by ELISAs (BioVendor). Human ELISAs. The plasma sample collected closest to study enrollment was measured using the following assays. Human SP-D (Cat # RD194059101) SP-A (Cat# RD191139200R) and CC16 (Cat# RD191022200) kits were obtained from BioVendor and ELISA measurements were performed as per manufacturer’s protocol. Plasma was diluted 11-fold for SP-D measurement and diluted 25-fold for CC16 measurement, and the final OD was measured at 450 nm using Synergy 2 plate reader from BioTek. Proteins are reported in absolute concentrations (pg/mL or ng/mL plasma).
Chakrabarti A., Nguyen A., Newhams M.M., Ohlson M.B., Yang X., Ulufatu S., Liu S., Park S., Xu M., Jiang J., Halpern W.G., Anania V.G., McBride J.M., Rosenberger C.M, & Randolph A.G. (2021). Surfactant Protein D is a Biomarker of Influenza-Related Pediatric Lung Injury. Pediatric pulmonology, 57(2), 519-528.
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5

Urine Albumin-Creatinine Ratio Analysis

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Urine was collected and the albumin concentration was measured with a mouse albumin ELISA (Bethyl laboratories, Montgomery, TX). A creatinine assay (R&D systems, Minneapolis, MN) was used to determine urine creatinine concentrations. Results are expressed as ACR in μg of albumin per mg of creatinine.
Dema B., Lamri Y., Pellefigues C., Pacreau E., Saidoune F., Bidault C., Karasuyama H., Sacré K., Daugas E, & Charles N. (2017). Basophils contribute to pristane-induced Lupus-like nephritis model. Scientific Reports, 7, 7969.
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6

Metabolic Biomarker Assays in Mice

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Blood glucose measurement and urinary albumin and creatinine assays were performed as described previously (18 (link)). Briefly, blood glucose was measured from tail vein blood by using a FreeStyle Freedom lite glucometer (Abbott, Abbott Park, IL). For urinary albumin and creatinine measurements, spot urine samples were collected non-invasively from mice. Urinary albumin and creatinine concentrations were measured using a mouse albumin ELISA (Bethyl Laboratories, Montgomery, TX) and a creatinine assay (Exocell, Philadelphia, PA), respectively. Subsequently, protein concentrations and urine albumin-to-creatinine ratios were calculated.
Li X., Venkatesh I., Villanueva V., Wei H., Geraghty T., Rajagopalan A., Helmuth R.W., Altintas M.M., Faridi H.M, & Gupta V. (2022). Podocyte-specific deletion of miR-146a increases podocyte injury and diabetic kidney disease. Frontiers in Medicine, 9, 897188.
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7

Quantifying Kidney Damage Biomarkers

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Urinary albumin was measured using mouse albumin ELISA (Bethyl Laboratories) and SDS-PAGE/Coomassie blue staining, as described elsewhere (16 (link)). Plasma and 24-h urine creatinine were measured by high-performance liquid chromatography (16 (link)). Transmission electron microscopy (TEM) was performed using standard techniques (16 (link)). Glomerular area was measured using ImageJ software (National Institutes of Health, Bethesda, MD; http://rsb.info.nih.gov/ij/) in 34 ± 2.1 glomeruli/kidney from 4 mice per experimental condition. A renal pathologist (G.M.) examined in a blinded fashion kidney specimens stained with periodic acid Schiff and assigned a semiquantitative pathology score based on the percentage of the area (0 = none; 1 = 1–25%; 2 = 26–50%; 3 = 51–75%; 4 = 76–100%) with the following features: glomerular nodules, mesangiolysis, mesangial sclerosis, and interstitial fibrosis (24 (link)). The percentage of glomeruli per section containing mesangiolysis or nodules was calculated (28 (link)).
Aggarwal P.K., Veron D., Thomas D.B., Siegel D., Moeckel G., Kashgarian M, & Tufro A. (2014). Semaphorin3a Promotes Advanced Diabetic Nephropathy. Diabetes, 64(5), 1743-1759.
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8

Histopathological Analysis of Kidney Injury

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To assess extent of tissue pathology kidneys were fixed by immersion in 10% formalin solution for 48h and embedded in paraffin. Fixed kidney sections were stained with hematoxylin and eosin and Periodic acid-Schiff reagent (to examine basement membrane). Stained kidney sections were scanned on a Hamamatsu Nano-zoomer to capture whole kidney images and analyzed using NDP Nanozoomer software. Glomerular and tubular pathology was scored as previously described 13 . To examine kidney function urine samples were collected over 24h and urinary albumin was measured by mouse albumin ELISA (Bethyl Labs).
Shinde R., Hezaveh K., Halaby M.J., Kloetgen A., Chakravarthy A., da Silva Medina T., Deol R., Manion K.P., Baglaenko Y., Eldh M., Lamorte S., Wallace D., Chodisetti S.B., Ravishankar B., Liu H., Chaudhary K., Munn D.H., Tsirigos A., Madaio M., Gabrielsson S., Touma Z., Wither J., De Carvalho D.D, & McGaha T.L. (2018). Apoptotic cell–induced, TLR9-dependent AhR activity is required for immunological tolerance and suppression of systemic lupus erythematosus in mice and humans. Nature immunology, 19(6), 571-582.
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9

Histopathological Analysis of Kidney Injury

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To assess extent of tissue pathology kidneys were fixed by immersion in 10% formalin solution for 48h and embedded in paraffin. Fixed kidney sections were stained with hematoxylin and eosin and Periodic acid-Schiff reagent (to examine basement membrane). Stained kidney sections were scanned on a Hamamatsu Nano-zoomer to capture whole kidney images and analyzed using NDP Nanozoomer software. Glomerular and tubular pathology was scored as previously described 13 . To examine kidney function urine samples were collected over 24h and urinary albumin was measured by mouse albumin ELISA (Bethyl Labs).
Shinde R., Hezaveh K., Halaby M.J., Kloetgen A., Chakravarthy A., da Silva Medina T., Deol R., Manion K.P., Baglaenko Y., Eldh M., Lamorte S., Wallace D., Chodisetti S.B., Ravishankar B., Liu H., Chaudhary K., Munn D.H., Tsirigos A., Madaio M., Gabrielsson S., Touma Z., Wither J., De Carvalho D.D, & McGaha T.L. (2018). Apoptotic cell–induced, TLR9-dependent AhR activity is required for immunological tolerance and suppression of systemic lupus erythematosus in mice and humans. Nature immunology, 19(6), 571-582.
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10

Urinary Albumin and Creatinine Assay

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Dilution factors for the albumin ELISA were determined by SDS-PAGE using 5 μl urine and a BSA standard; staining was with PageBlue™ (Thermo Fisher Scientific, Waltham, MA, USA). Urinary albumin and creatinine levels were determined by using the Mouse Albumin ELISA (E99-134, Bethyl Laboratories, Montgomery, TX, USA) and Creatinine Companion (1012, Ethos Biosciences, Philadelphia, PA, USA) kits according to the manufacturers' protocols. Albuminuria was computed as a ratio of urinary albumin to creatinine.
McCarthy G.M., Blasio A., Donovan O.G., Schaller L.B., Bock-Hughes A., Magraner J.M., Suh J.H., Tattersfield C.F., Stillman I.E., Shah S.S., Zsengeller Z.K., Subramanian B., Friedman D.J, & Pollak M.R. (2021). Recessive, gain-of-function toxicity in an APOL1 BAC transgenic mouse model mirrors human APOL1 kidney disease. Disease Models & Mechanisms, 14(8), dmm048952.
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