Fmoc glu otbu oh

The gold electrodes were purchase from DropSens (Asturias, Spain) and BASi (West Lafayette, IN, USA), Rink amide resin (0.46 meq/g), Fmoc-Arg(Pbf)-OH, Fmoc-Lys(Boc)-OH, Fmoc-Glu(OtBu)-OH, Fmoc-Ala-OH, Fmoc-Ile-OH, Fmoc-Thr(tBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Asn(Trt)-OH, Fmoc-Pro-OH, Fmoc-Cys(Trt)-OH, Fmoc-His(Trt)-OH, Fmoc-Lys(ivDde)-OH, Fmoc-Lys(Fmoc)-OH, 6-Fmoc-aminohexanoic acid (Fmoc-Ahx-OH), Fmoc-Tyr(tBu)-OH, 1-hydroxybenzotriazole (HOBt), and N,N-dicyclohexylcarbodiimide (DCC) were purchased from AAPPTec (Louisville, KY, USA). N,N-diisopropylethylamine (DIPEA), triisopropylsilane (TIPS), 1,2-ethanedithiol (EDT), acetic anhydride, piperidine, pyridine, ninhydrin, phenol, ferrocene, ferrocenecarboxylic acid, Freund’s incomplete and complete adjuvant, and KCN were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol, diethyl ether, N,N-dimethylformamide (DMF), absolute ethanol, dichlorometane (DCM), acetonitrile (AcN), isopropyl alcohol (IPA), and trifluoroacetic acid (TFA) were obtained from Honeywell-Burdick & Jackson (Muskegon, MI, USA). Analytical grade KOH, KCl, Ru(NH₃)₆Cl₃, K₃Fe(CN)₆, and H₂SO₄ were acquired from J.T. Baker. The pH was adjusted with either KOH or HCl solutions obtained from J. T. Baker. All aqueous solutions were prepared with deionized water (ρ ≥ 18 MΩ cm⁻¹). All reagents were used without further purification.

Mueller-Hinton, Agar SPC, E. coli ATCC 25922, and E. faecalis ATCC 29212 were obtained from ATCC, USA. Rink amide resin, Fmoc-Arg(Pbf)-OH, Fmoc-Asp(OtBu)-OH, Fmoc-Lys(Boc)-OH, Fmoc-Trp(Boc)-OH, Fmoc-Gln(Trt)-OH, Fmoc-β-Ala-OH, Fmoc-Phe-OH, Fmoc-Met-OH, Fmoc-Leu-OH, Fmoc-Gly-OH, Fmoc-Ala-OH, Fmoc-Ile-OH, Fmoc-Glu(OtBu)OH, Fmoc-Thr(tBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Val-OH, Fmoc-Pro-OH, Fmoc-Cys(Trt)-OH, Fmoc-Lys(Fmoc)-OH, 6-(Fmoc-amino)hexanoic acid (Fmoc-Ahx-OH), 1-hydroxybenzotriazole (HOBt), and N,N-dicyclohexylcarbodiimide (DCC) were purchased from AAPPTec (Louisville, KY, USA). N,N-Diisopropylethylamine (DIPEA), triisopropylsilane (TIPS), 1,2-Ethanedithiol (EDT), 4-methyl-piperidine, pyridine, ninhydrin, phenol, and KCN were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol, diethyl ether, N,N-dimethylformamide (DMF), absolute ethanol, dichloromethane (DCM), acetonitrile (ACN), isopropyl alcohol (IPA), and trifluoroacetic acid (TFA) were obtained from Honeywell-Burdick & Jackson (Muskegon, Michigan, USA). All reagents were used without further purification.

The Rink amide resin and Fmoc-Gly-OH, Fmoc-Ala-OH, Fmoc-Val-OH, Fmoc-Leu-OH, Fmoc-Ile-OH, Fmoc-Phe-OH, Fmoc-Tyr(tBu)-OH, Fmoc-Trp(Boc)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Thr(tBu)-OH, Fmoc-Cys(Trt)-OH, Fmoc-Met-OH, Fmoc-Asp(OtBu)-OH, Fmoc-Glu(OtBu)-OH, Fmoc-His(Trt)-OH, Fmoc-Lys(Boc)-OH, Fmoc-Arg(Pbf)-OH, Fmoc-Asn(Trt)-OH, Fmoc-Gln(Trt)-OH, Fmoc-Pro-OH, Fmoc-Ahx, N,N-Dicyclohexylcarbodiimide, and 1-Hydroxy-6-chlorobenzotriazole were purchased from AAPPTec (Louisville, KY, USA). The reagents such as, acetonitrile, trifluoroacetic acid, dichloromethane, diisopropylethylamine, N, N-dimethylformamide, ethanedithiol, isopropanol, methanol, and triisopropylsilane were purchased from Merck (Darmstadt, Germany). Supelclean^TM SPE columns were purchased from Sigma-Aldrich (St. Louis, MO, USA), and Silicycle^® SiliaPrep^TM C18 columns were kindly donated by EcoChem Especialidades Químicas (Waterloo, QC, Canada).