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Hspa8 antibody

Manufactured by Cell Signaling Technology
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The HSPA8 antibody is a research-use only product designed to detect the HSPA8 protein, which is a member of the heat shock protein 70 (Hsp70) family. HSPA8 functions as a molecular chaperone, assisting in the folding and transport of other proteins within the cell. This antibody can be used in various immunoassay applications to study the expression and localization of HSPA8 in biological samples.

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Lab products found in correlation

Nlrc4 antibody, by Merck Group (2 mentions) Pro caspase1 antibody, by Cell Signaling Technology (2 mentions) Cleaved caspase 1 antibody, by Cell Signaling Technology (2 mentions) Horseradish peroxidase hrp conjugated secondary antibody, by Cell Signaling Technology (2 mentions)

Close spelling variants of this product

HSPA8

2 protocols using hspa8 antibody

1

NLRC4 and Caspase-1 Activation in THP1 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
5×105 transduced THP1 cells were subjected to SDS-PAGE, transferred to nitrocellulose membranes and probed with appropriate antibodies. Antibodies used for protein blots were NLRC4 antibody (1:500 dilution; Millipore, 06-1125), pro-caspase1 antibody (1:1000 dilution; Cell Signaling Technology, 2225), cleaved caspase-1 antibody (1:500 dilution; Cell Signaling Technologies, 4199), HSPA8 antibody (1:2000 dilution; Cell Signaling Technologies, 8444). Horseradish peroxidase (HRP)-conjugated secondary antibodies (Cell Signaling Technologies, 7074) and SuperSignal West Pico chemiluminescent substrate (Pierce) were used to detect primary antibodies. Equal protein loading was assessed by immunoblotting for HSPA8.
Canna S.W., de Jesus A.A., Gouni S., Brooks S.R., Marrero B., Liu Y., DiMattia M.A., Zaal K.J., Montealegre Sanchez G.A., Kim H., Chapelle D., Plass N., Huang Y., Villarino A.V., Biancotto A., Fleisher T.A., Duncan J.A., O’Shea J.J., Benseler S., Grom A., Deng Z., Laxer R.M, & Goldbach-Mansky R. (2014). An activating NLRC4 inflammasome mutation causes autoinflammation with recurrent macrophage activation syndrome. Nature genetics, 46(10), 1140-1146.
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2

NLRC4 and Caspase-1 Activation in THP1 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
5×105 transduced THP1 cells were subjected to SDS-PAGE, transferred to nitrocellulose membranes and probed with appropriate antibodies. Antibodies used for protein blots were NLRC4 antibody (1:500 dilution; Millipore, 06-1125), pro-caspase1 antibody (1:1000 dilution; Cell Signaling Technology, 2225), cleaved caspase-1 antibody (1:500 dilution; Cell Signaling Technologies, 4199), HSPA8 antibody (1:2000 dilution; Cell Signaling Technologies, 8444). Horseradish peroxidase (HRP)-conjugated secondary antibodies (Cell Signaling Technologies, 7074) and SuperSignal West Pico chemiluminescent substrate (Pierce) were used to detect primary antibodies. Equal protein loading was assessed by immunoblotting for HSPA8.
Canna S.W., de Jesus A.A., Gouni S., Brooks S.R., Marrero B., Liu Y., DiMattia M.A., Zaal K.J., Montealegre Sanchez G.A., Kim H., Chapelle D., Plass N., Huang Y., Villarino A.V., Biancotto A., Fleisher T.A., Duncan J.A., O’Shea J.J., Benseler S., Grom A., Deng Z., Laxer R.M, & Goldbach-Mansky R. (2014). An activating NLRC4 inflammasome mutation causes autoinflammation with recurrent macrophage activation syndrome. Nature genetics, 46(10), 1140-1146.
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