The rabbit anti-Bcl-XL, the rabbit anti-caspase-3, the rabbit anti-LC3, the mouse anti-PARP1, the rabbit anti-actin antibodies were from Cell Signaling (ref. no. 2764, 9662, 2775, 9546, 4970 respectively). The rabbit anti-ATG6, the rabbit anti-total Bax and the rabbit anti-total Bak were from Santa Cruz Biotechnology (ref. no. sc-11427, sc-493 and sc-832 respectively). The mouse anti-caspase-1 was from Adipogen (ref. no. AG-20B-0048). The rabbit anti-ATG5 was from Abcam (ref. no. ab108327). The rat anti-MLKL was from Merck Millipore (ref. no. MABC604). The mouse FITC-labelled anti-CD19 was from Beckman Coulter (ref. no. A07768).
Anti mouse caspase 1
Manufactured by Adipogen
Sourced in United States
Anti-mouse caspase-1 is a laboratory reagent that specifically binds and detects mouse caspase-1 protein. Caspase-1 is an enzyme involved in the inflammatory response and cell death processes. This product can be used in various immunological and cell biology applications to study the role of caspase-1 in mouse models.
Automatically generated - may contain errors
Lab products found in correlation
Adenosine triphosphate (atp), by Merck Group (12 mentions)
Nigericin, by Merck Group (10 mentions)
Ag 20b 0042, by Adipogen (9 mentions)
Mouse anti il 1β, by Cell Signaling Technology (8 mentions)
Sc 22514 r, by Santa Cruz Biotechnology (7 mentions)
Anti asc, by Santa Cruz Biotechnology (7 mentions)
Mcc950, by Targetmol (7 mentions)
Anti nlrp3, by Cell Signaling Technology (7 mentions)
Anti gapdh, by Proteintech (7 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (7 mentions)
Goat anti il 1β, by R&D Systems (5 mentions)
Icariin, by Targetmol (5 mentions)
Anhydroicaritin, by Targetmol (5 mentions)
Epimedin a, by Targetmol (5 mentions)
Epimedin a1, by Targetmol (5 mentions)
Epimedin b, by Targetmol (5 mentions)
Epimedin c, by Targetmol (5 mentions)
Lps escherichia coli 055 b5, by Merck Group (5 mentions)
Anti nlrp3, by Adipogen (4 mentions)
Pam3csk4, by InvivoGen (4 mentions)
22 protocols using anti mouse caspase 1
1
Antibody Reagent Identification for Cell Analysis
2
Immunofluorescence Imaging of Cellular Markers
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Frozen brain sections or cells were fixed with 4% PFA for 20 min at room temperature and were permeabilized with 1% Triton X‐100 (Solarbio, Beijing, China) solution for 15 min. Next, 5% BSA in PBS with 0.1% triton was used to block non‐specific binding sites at room temperature for 1 h. The slides were incubated with the following corresponding primary antibodies at 4°C overnight: mouse anti‐dsDNA (1:100, Santa Cruz Biotechnology); rabbit anti‐53BP1 (1:5,000, Novus Biologicals); rabbit anti‐Iba1 (1:500, Wako Pure Chemical Industries, Ltd., Japan); rabbit anti‐NeuN (1:500, Abcam); goat anti‐GFAP (1:500, Abcam); rat anti‐Ly6G (1:100, BioLegend); mouse anti‐cGAS (1:100, Santa Cruz Biotechnology); mouse anti‐GSDMD (1:100, Santa Cruz Biotechnology); mouse anti‐caspase‐1 (1:100, Adipogen); and goat anti‐IL‐1β (1:100, R&D Systems). The following day, the sections were washed with cold PBS; the immunoreactions were visualized using fluorescent secondary antibodies. Nuclei were costained with Fluoroshield Mounting Medium containing DAPI (104139, Abcam). All the slides were visualized and photo‐documented using a confocal microscope (Olympus, Heidelberg, Germany) or a Nikon Coolscope digital microscope (Nikon, Tokyo, Japan), and quantified by ImageJ software (NIH).
3
Inflammasome and α-Synuclein Regulation
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Dulbecco's Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS) and other cell culture reagents, goat anti-mouse- AlexaFluor594 (H + L) antibody, goat anti-rabbit-AlexaFluor488 (H + L) antibody and rabbit IgG isotype control antibody were obtained from Thermo Fisher Scientific (Invitrogen). si-m-PYCARD/ASC RNAs were obtained from Guangzhou RiboBio Co., Ltd. Mouse anti-NLRP3 (Cat# AG-20B-0014), mouse anti-caspase-1 (Cat# AG-20B-0042) and rabbit anti-ASC (Cat# AG-25B-0006) were purchased from AdipoGen Life Sciences. Rabbit anti-α-synuclein (Cat# 4179), rabbit anti-Phospho-α-synuclein (Cat# 23706), rabbit anti-phospho-NF-κB p65 (Cat# 3033) and mouse anti-IL-1β (Cat# 12242) were purchased form Cell Signaling Technology. rabbit anti-ASC (Cat# YT0365) was purchased from ImmunoWay Biotechnology. Mouse anti-Phospho-α-synuclein (Cat# pSyn #64) and rabbit anti-IBA1 (Cat# 019-19741) were purchased from FUJIFILM Wako Pure Chemical Corporation. Mouse anti-α-synuclein (Cat# sc-12767) and mouse anti-GSDMD (Cat# sc-393581) was purchased from Santa Cruz Biotechnology. Rabbit anti-NF-κB p65 (Cat# ab16502), goat anti-IBA1 (Cat# ab5076) and rabbit anti-GSDMD (Cat# ab219800) were purchased from Abcam. Rabbit anti-IL18 (Cat# A1115) was purchased from ABclonal. Rabbit anti-tyrosine hydroxylase (Cat# AB152) was purchased from Merck Millipore.
4
Immunohistochemical Analysis of Inflammatory Markers
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Immunohistochemical analysis was performed as previously described (McKenzie et al, 2018 ). Frozen sections (8 μm) were incubated with 0.3% hydrogen peroxide for 20 min to inactivate endogenous peroxidases. After washing in PBS, brain sections were blocked with 5% bovine serum albumin (BSA) for 1 h at room temperature. Thereafter, brain sections were incubated 4°C overnight with the following primary antibodies: mouse anti‐cGAS (1:100, Santa Cruz Biotechnology); mouse anti‐caspase‐1 (1:100, Adipogen); goat anti‐IL‐1β (1:100, R&D Systems); and mouse anti‐GSDMD (1:100, Santa Cruz Biotechnology). Slides were then washed in PBS and incubated with suitable biotinylated secondary antibodies (1: 500, Vector Laboratories) for 2 h at room temperature. The sections were followed by incubation with avidin–biotin–peroxidase complex (VECTASTAIN Elite ABC Kit; Vector Laboratories). The immunoreactivity was visualized with 3,3′‐diaminobenzidine (DAB). A set of sections was also stained in a similar way but without the primary antibody and served as the negative control. Sections were then mounted in neutral balsam. All slides were imaged using a Nikon Coolscope digital microscope (Nikon, Tokyo, Japan).
5
Immunohistochemical Detection of Caspase-1
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Mouse lung sections were deparaffinized and rehydrated in an ethanol series. The sections were treated with 1.4% H2O2 in methanol for 30 minutes to block endogenous peroxidase; they were then non-specific binding blocked using 1.5% horse serum and incubated with mouse anti-caspase-1 (1:2,000; Adipogen). The following day, sections were incubated with an ABC kit (Vector Laboratories, Burlingame, CA, USA). Color reaction was developed using a liquid DAB+ substrate kit (Golden Bridge International Inc., Mukilteo, WA, USA). After immunohistochemical staining, the slides were counterstained with Herris's hematoxylin for 1 minute. Expression was quantified using the ImageJ software (National Institutes of Health, Bethesda, MD, USA).
6
Immunofluorescence Imaging of Neuroinflammatory Markers
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Cells were cultured on poly-L-lysine coated coverslips and fixed with paraformaldehyde (4% in PBS) after stimulation. Cells were incubated overnight at 4°C with Cy3-labeled mouse anti-GFAP (1:800, Sigma-Aldrich), rabbit anti-Iba1 (1:200, Biocare Medical), goat anti-IL-1β (1:100, R&D), mouse anti-NLRP3 (1:100), rabbit anti-ASC (1:200), mouse anti-Caspase-1 (1:300, all three Adipogen) and rabbit anti-HMGB1 (1:400, Abcam). After washing with PBS, cells were incubated with anti-rabbit, anti-goat or anti-mouse secondary antibodies coupled to either Cy3 or AlexaFluor 488 (Invitrogen). Cells were then washed with PBS and mounted with DAPI-Fluoromount G (SouthernBiotech, USA), and observed under a LSM 510 META inverted confocal microscope (Carl Zeiss Micro Imaging, Göttingen, Germany).
7
Antibody-based Protein Detection Assay
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Antibodies used were rabbit anti-tankyrase 1/2 and mouse anti-Bcl-2 (Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-podocin and mouse anti-tubulin (Sigma-Aldrich), goat anti-tankyrase 2, rabbit anti-BAX and rabbit anti-fibronectin (Abcam, Cambridge, UK), rabbit anti-CD2AP 1774 or 1764,4 (link), 51 (link) mouse anti-PARP1 and rabbit anti-Poly(ADP-ribose) (Enzo Life Sciences, Farmingdale, NY, USA), mouse anti-active-β-catenin (Millipore, Billerica, MA, USA), rabbit anti-LEF1 and rabbit anti-phospho p38 MAPK (Cell Signaling Technology, Danvers, MA, USA) and mouse anti-WT1 (Upstate, Lake Placid, NY, USA), guinea pig anti-p62/SQSTM1 (Progen Biotechnik GmbH, Heidelberg, Germany) and mouse anti-Caspase-1 (Adipogen, San Diego, CA, USA).
8
Immunoblotting for Signaling Proteins
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The immunoblotting analyses were conducted as described previously [14] . The specimens were electrophoresed with 12% SDS-polyacrylamide gels and transferred to nitrocellulose membranes by electrophoresis. Following the blocking, the membranes were incubated at 4 °C overnight under gentle agitation with each primary antibody: goat anti-IL-1β (1:1000, R&D), mouse anti-Caspase-1 (1:1000; AdipoGen), goat anti-CatB (1:1000; Santa Cruz Biotechnology), mouse anti-IκBα (1:1000; Cell Signaling), rabbit anti-Synaptophysin (1:20000; Abcam), rabbit anti-Phospho-Akt (Thr308, 1:1000, Cell Signal), rabbit anti-Phospho-Akt (Ser473, 1:1000, Cell Signal), rabbit anti-total Akt (1:1000, Cell Signal), rabbit anti-Phospho-CREB (1:1000, Cell Signal), rabbit anti-total CREB (1:1000, Cell Signal). After washing, the membranes were incubated with HRP-labeled anti-goat (1:2000; GE Healthcare), anti-mouse (1:2000; R&D Systems), anti-rabbit (1:2000; GE Healthcare), and mouse anti-actin (1:5,000, Abcam) for 2 hours at room temperature. After that, the HRP-labeled protein bands were detected by an enhanced chemiluminescence detection system (ECL kit; GE Healthcare) with an image analyzer (LAS-1000; Fuji Photo Film).
9
Immunoblotting Analysis of Inflammation Markers
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The immunoblotting analyses were conducted as described previously [14] . The specimens were electrophoresed with 12% SDS-polyacrylamide gels and transferred to nitrocellulose membranes by electrophoresis. Following the blocking, the membranes were incubated at 4°C overnight under gentle agitation with each primary antibody: goat anti-IL-1β (1:1000, R&D), mouse anti-Caspase-1 (1:1000; AdipoGen), goat anti-CatB
10
Inflammasome Activation and Inhibition
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Paclitaxel, doxorubicin, etoposide, LPS, ATP, nigericin, staurosporine, glibenclamide, and valiomycin were purchased from Sigma-Aldrich. Ciliobrevin D and SP600125 were purchased from Calbiochem. JC-1 and MitoSOX were purchased from Invitrogen. Alum was purchased from InvivoGen. Anti-mouse caspase-1, anti-NLRP3 and anti-ASC antibodies were obtained from AdipoGen. Anti-mouse IL-1β antibody was obtained from R&D Systems. Anti-phospho-IκB and anti- IκB antibodies were purchased from Cell Signaling Technology. Anti-phospho-JNK and anti-JNK antibodies were obtained from Invitrogen and BD, respectively. Anti-β-actin antibody was purchased from Santa Cruz Biotechnology.
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