Lentinan (molecular weight, 36,000–50,000) was purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). The antibiotics ampicillin, neomycin sulfate, metronidazole and vancomycin were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). The broad-spectrum antibiotics solution was comprised of four antibiotics (ampicillin (100 mg/kg, Macklin Biochemical), vancomycin (50 mg/kg, Macklin Biochemical), metronidazole (100 mg/kg, Macklin Biochemical), neomycin sulfate (100 mg/kg, Macklin Biochemical)) and made fresh every 24 h.
Vancomycin
Manufactured by Maclin Biochemical Technology
Sourced in China
Vancomycin is a laboratory equipment used for the detection and quantification of the antibiotic compound vancomycin. It is a high-performance liquid chromatography (HPLC) based instrument designed to analyze the concentration of vancomycin in various samples, such as biological fluids or pharmaceutical preparations.
Automatically generated - may contain errors
Lab products found in correlation
Ampicillin, by Maclin Biochemical Technology (14 mentions)
Metronidazole, by Maclin Biochemical Technology (14 mentions)
Neomycin sulfate, by Maclin Biochemical Technology (10 mentions)
Neomycin, by Maclin Biochemical Technology (3 mentions)
Colistin, by Maclin Biochemical Technology (2 mentions)
Ciprofloxacin, by Maclin Biochemical Technology (1 mentions)
Amikacin, by Maclin Biochemical Technology (1 mentions)
Amoxicillin, by Maclin Biochemical Technology (1 mentions)
Aztreonam, by Maclin Biochemical Technology (1 mentions)
Meropenem, by Maclin Biochemical Technology (1 mentions)
Rifampicin, by Maclin Biochemical Technology (1 mentions)
Sulbactam, by Maclin Biochemical Technology (1 mentions)
Polymyxin b, by Maclin Biochemical Technology (1 mentions)
Aluminum, by Merck Group (1 mentions)
Ovalbumin (ova), by Merck Group (1 mentions)
Mouse anti dnp ige monoclonal antibody, by Merck Group (1 mentions)
Dnp hsa, by Merck Group (1 mentions)
Axyprep dna gel extraction kit, by Corning (1 mentions)
Fastdna spin kit for soil, by MP Biomedicals (1 mentions)
Lentinan, by Solarbio (1 mentions)
22 protocols using vancomycin
1
Lentinan and Broad-Spectrum Antibiotic Preparation
2
Antibiotic Modulation of Intestinal Inflammation in Apc-Mutant Mice
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ApcMin/+ mice were pretreated with an antibiotics (ABX) mixture containing 500 mg/l vancomycin (Cat# V820413), 1 g/l ampicillin (Cat# A6265), 1 g/l metronidazole (Cat# M813526) and 1 g/l neomycin (Cat# N814740) (VAMN, Macklin Inc., Shanghai, China) for 3 weeks with addition of 75 g/l sucrosein drinking water, and then were co-administrated with DSS for 1 week, and were continuously treated with ABX for further 4 weeks. In some experiment, ABX cocktail containing 0.4 g/l kanamycin (Cat# K812216), 0.035 g/l gentamycin (Cat# G6064), 0.0570 g/l colistin (Cat# C805491), 0.215 g/l metronidazole, 0.045 g/l vancomycin HCl and 0.025 g/l erythromycin (Cat# E808820)68 (link) (6 mixture, Macklin) were used to treat mice.
3
Fecal Microbiota Transfer in Sleep-Deprived Mice
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Modified according to the previous literature [16 (link),17 (link)], the feces of sleep-deprived mice were collected once every other day, all feces were pooled together, and then homogenized at 100 mg/mL and supplemented with saline (Solarbio Science & Technology Co., Ltd., Beijing, China). The homogenized solution was centrifuged at 500 rpm for 10 min, and the supernatant was the bacterial suspension. An amount of 100 μL/10 g of the bacterial suspension was transplanted into normal sleeping mice via oral gavage three times a week for 6 weeks. Before FMT, recipient mice were treated with 200 μL of an antibiotic mixture (ampicillin (Shanghai Macklin Biochemical Co., Ltd., Shanghai, China), 1 g/L; metronidazole (Shanghai Macklin Biochemical Co., Ltd., Shanghai, China), 1 g/L; neomycin (Shanghai Macklin Biochemical Co., Ltd., Shanghai, China), 1 g/L; vancomycin (Shanghai Macklin Biochemical Co., Ltd., Shanghai, China), 0.5 g/L) three times, one day apart. FMT was stopped two days before dissection. Fecal samples from the donor and recipient were collected in a sample stabilizer and cryopreserved for subsequent gut microbiota analysis.
4
Antibiotic Cocktail Treatment in Mice
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C57BL/6J mice (male, 6–8 weeks) were treated with antibiotics according to the previously published protocol [44 (link)]. Briefly, the antibiotic compounds were applied via drinking water for 7 weeks and consisted of ampicillin (1 g/l, Meryer, Shanghai, China), vancomycin (500 mg/l, Macklin, Shanghai, China), ciprofloxacin (200 mg/l, Macklin, Shanghai, China), imipenem plus cilastatin (250 mg/l, MSD, Kenilworth, NJ, USA), and metronidazol (1 g/l, Aladdin, Shanghai, China). Antibiotic water bottles were inverted every day, and antibiotic solution was changed every 2–3 days.
5
Antibiotic Stock Solutions Preparation
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Analytical-grade amikacin, amoxicillin, aztreonam, clindamycin, colistin, polymyxin-B, meropenem, rifampicin, sulbactam, and vancomycin were obtained from the Shanghai Macklin Biochemical Co. Ltd. (Shanghai, China). Stock solutions containing each antibiotic were separately prepared at a concentration of 10.24 mg/l according to the Clinical Laboratory and Standards Institute guidelines (CLSI, 2020 ).
6
Allergy-inducing Mouse Model Protocols
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Metronidazole, ampicillin, neomycin sulfate, and vancomycin were obtained from Macklin Biochemical Technology (Shanghai, China). Mouse anti-DNP-IgE monoclonal antibody, DNP-HSA, ovalbumin (OVA), cholera toxin (CT), and aluminum were obtained from Sigma-Aldrich. Mouse IL-4, IL-5, IFN-γ, TNF-α and mMCP-1, histamine, zonluin, and the sIgA ELISA kit were from Dongge Boye (Beijing, China). Mouse ZO-1, Claudin-1, and Occludin polyclonal antibodies were obtained from Lianke Biotechnology (Hangzhou, China). The FastDNA SPIN Kit for soil was from Mpbio (Santa Ana, CA, USA). PicoGrenn dsDNA was from Life Technology. The AXYPREP DNA Gel Extraction Kit was obtained from Axygen Biosciences. The Roche GS FLX Titanium em PCR Kit and Roche GS FLX Sequencing kit were from Roche Applied Science. DNA DL2000Marker was obtained from Takara Bio (Dalian, China).
7
Inducing Lipogenesis and Gut Microbiota Depletion in Chickens
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The HFD group of chickens was subjected to a diet comprising basal feed and soybean oil (20%) for a duration of 4 wk, with the aim of inducing excessive lipogenesis and fat deposition. On the other hand, the ABX group of chickens was administered a mixture of 4 antibiotics via oral gavage for a period of 21 d, with the objective of depleting the gut microbiota. Each chicken was housed in an individual cage and provided with ad libitum access to food and water. The antibiotic mix consists of vancomycin (Macklin, Shanghai, China, 0.5 μg/mL), Ampicillin (Macklin, Shanghai, China, 1 μg/mL), Neomycin sulfate (Macklin, Shanghai, China, 1 μg/mL), and Metronidazole (Macklin, Shanghai, China, 1 μg/mL). Chickens in the ABX group were provided with ad libitum access to the water with 4 antibiotics.
8
Inflammatory Bowel Disease Cytokine Profile Assay
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Reference standards, including caffeic acid, quercetin, isoquercitrin, ferulic acid, vitexin, vicenin II, schaftoside, isoschaftoside, rutin, hyperoside, salicylic acid, rosmarinic acid, daidzein, oleanolic acid, stigmasterol, epinodosin, methyl rosmarinate, kamebakaurin, shikokianin, lasiokaurin, oridonin, lasiodonin, ponicidin, and nodosin were purchased from Refmedic Biotechnology Co., Ltd. (Chengdu, China). Additionally, 5-ASA was purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China), while DSS (molecular weight: 36–50 kDa) was obtained from International Laboratory USA (San Francisco, CA, USA). The antibiotics, namely, vancomycin, neomycin sulfate, ampicillin, and metronidazole, were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Mouse serum interleukin (IL)-17, IL-4, transforming growth factor (TGF)-β1, and interferon (IFN)-γ enzyme-linked immunosorbent assay (ELISA) kits were obtained from Novus Biologicals (Littleton, CO, USA). A lipopolysaccharide (LPS) ELISA kit was provided by CUSABIO (Wuhan, China). A fixation/permeabilization solution kit (BD GolgiPlug) and CD25, Foxp3, CD4, and IL-17A antibodies used for Th17 and Treg cell phenotyping were purchased from BD Biosciences (Franklin Lakes, NJ, USA).
9
Compound Characterization and Synthesis
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EA powder (purity ≥ 99%), urolithin C (purity ≥ 98%), Uro-A (purity ≥ 99%), and amphotericin B were purchased from Sigma (Sigma-Aldrich, St. Louis, MO, USA). Vancomycin, polymyxin B sulfate, ethyl acetate, and liquid paraffin were purchased from Macklin Biochemical (Shanghai, China). Formic acid and acetonitrile were purchased from Aladdin Chemical Company (Shanghai, China), and brain heart leachate broth (BHI) was purchased from OXOID (Basingstoke, UK). All other chemicals used are of analytical and chromatographic grade.
10
Antibiotic Susceptibility Assay Protocol
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Ampicillin (98%, Shanghai Macklin Biochemical Co., Ltd.), neomycin sulfate (United States Pharmacopoeia, (USP) grade, 680 I.U./mg, Shanghai Macklin Biochemical Co., Ltd.), metronidazole (99%, Shanghai Macklin Biochemical Co., Ltd.), vancomycin (USP, ≥950 μg/mg, Shanghai Macklin Biochemical Co., Ltd.), glycerol (Sangon Biotech (Shanghai) Co., Ltd.), biosafety cabinet (HR30/IIA2, Qingdao Haier Biomedical Co., Ltd.), electronic balance (BSA2202S, Sartorius AG), frozen centrifuge (X1R 75,004,250, Thermo Fisher Scientific, China), anaerobic incubator (BACTRON EZ/2, Shanghai Longyue Instrument Equipment Co., Ltd.), and cryogenic freezer (DW-86 L626, Haier Biomedical Co., Ltd.) were used.
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