Total proteins were obtained using radioimmunoprecipitation assay buffer (cat. no. 89900; Invitrogen; Thermo Fisher Scientific, Inc.), and determined using a bicinchoninic acid kit (cat. no., P0010; Beyotime Institute of Biotechnology). Proteins (50 µg) were separated using 10% SDS-PAGE. After membranes were blocked with 5% non-fat milk at 37°C for 1 h, the membranes were incubated with primary antibodies against FSCN1 (1:1,200; cat. no. ab220195; Abcam) and GAPDH (1:2,000; cat. no: ab181602; Abcam), followed by HRP-conjugated goat anti-mouse IgG secondary antibody (1:2,000; cat. no. ab97040; Abcam) overnight at 4°C. Finally, bands were visualized using a FluorChem imaging device (ProteinSimple). Band intensities were analyzed using ImageJ (version 1.45; National Institutes of Health).
Hrp conjugated goat anti mouse igg secondary antibody
Manufactured by Abcam
Sourced in United Kingdom
HRP-conjugated goat anti-mouse IgG secondary antibody is a laboratory reagent used to detect and visualize mouse immunoglobulin G (IgG) in various immunoassays. The antibody is conjugated with horseradish peroxidase (HRP), an enzyme that can catalyze a color-producing reaction, allowing for the detection and quantification of target mouse IgG molecules.
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Lab products found in correlation
2 protocols using hrp conjugated goat anti mouse igg secondary antibody
1
Protein Quantification and Western Blot Analysis
2
ELISA for Anti-BoHV-1 Antibody Detection
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The enzyme-linked immunosorbent assay (ELISA) was employed to identify anti-BoHV-1 antibody levels. BoHV-1 Cooper strain was purified by ultracentrifugation and then inactivated by ultrasonication (28 kHz, 600 W) for 30 min [14 (link)]. The inactivated viruses were used as coating antigens. The ELISA was established in our laboratory. Briefly, 100 ng of antigens were coated in each well of a 96-well plate at 37 °C for 1 h and followed by blocking with 1% BSA at 37 °C for 2 h. The mouse sera were inoculated at 37 °C for 1 h and followed by three washes with PBST. The HRP-conjugated goat anti-mouse IgG secondary antibody (Abcam, UK) was used to detect the anti-BoHV-1 antibody level. The serum antibody level was monitored per week after the second vaccination.
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