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Ph 6.0 citrate buffer

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The PH 6.0 citrate buffer is a laboratory solution designed to maintain a specific pH level of 6.0. It is a common buffer used in various scientific applications to control the acidity or basicity of a solution. The citrate buffer is composed of citric acid and sodium citrate, which together provide a stable pH environment. This product is intended for use in research, analytical, and quality control settings where precise pH control is required.

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Lab products found in correlation

Vs120 slide scanner, by Olympus (3 mentions) Hematoxylin, by Merck Group (3 mentions) Anti erbb2 antibody, by Agilent Technologies (2 mentions) Anti s100a1 antibody, by Agilent Technologies (2 mentions) Anti cleaved caspase 3 asp175 antibody, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Citrate buffer (163 citations) Citrate buffer pH 6 (21 citations)

4 protocols using ph 6.0 citrate buffer

1

Immunohistochemical Analysis of Mouse Xenograft Tumors

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Formalin-fixed tumor samples derived from mouse xenografts were processed and embedded in paraffin. Tissue sections were deparaffinized and antigen retrieval was achieved by use of heat-induced epitope retrieval with pH 6.0 citrate buffer (Sigma). Tissue sections were stained with H&E, anti-ERBB2 antibody (Dako, A0485, dilution 1:200), or anti-S100A1 antibody (Dako, Z031129-2, dilution 1:00). Immunostained slides were counterstained with hematoxylin (Sigma). Images of tissue sections were captured at 10× magnification to create a merged image of the entire section using Olympus VS120 Slide Scanner.
Naffar-Abu Amara S., Kuiken H.J., Selfors L.M., Butler T., Leung M.L., Leung C.T., Kuhn E.P., Kolarova T., Hage C., Ganesh K., Panayiotou R., Foster R., Rueda B.R., Aktipis A., Spellman P., Ince T.A., Xiu J., Oberley M., Gatalica Z., Navin N., Mills G.B., Bronson R.T, & Brugge J.S. (2020). Transient commensal clonal interactions can drive tumor metastasis. Nature Communications, 11, 5799.
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2

Immunofluorescence Staining Protocol with Antigen Retrieval

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For immunofluorescence staining, antigen retrieval was done by water bath incubation with pH 6.0 citrate buffer (Sigma-Aldrich) at 95 oC for 35 min. Samples were quenched by incubation with 0.1% Sudan black B, diluted in 70% ethanol for 25 min. Primary antibodies were incubated at 4 oC overnight whereas Alexa Fluor (AF) conjugated secondary antibodies diluted in blocking serum were incubated at room temperature for 1 h (antibodies listed in Supplementary Table 13). Wheat germ agglutinin (WGA) staining was performed with 5 μl ml−1 AF 647 conjugate (Invitrogen, W32466) before incubation with protein block serum-free (Dako). All sections were incubated with 300 nM DAPI stain, mounted with Vectashield Antifade mounting medium and cured for 24 h. Visualization of fluorophores was by TissueFAXS SL Q + upright epifluorescence microscope and viewed with TissueFAXS slide viewer 7.0 software. FIJI ImageJ software v1.8.0_72 was used to prepare the images for presentation.
Wu X., Azizan E.A., Goodchild E., Garg S., Hagiyama M., Cabrera C.P., Fernandes-Rosa F.L., Boulkroun S., Kuan J.L., Tiang Z., David A., Murakami M., Mein C.A., Wozniak E., Zhao W., Marker A., Buss F., Saleeb R.S., Salsbury J., Tezuka Y., Satoh F., Oki K., Udager A.M., Cohen D.L., Wachtel H., King P.J., Drake W.M., Gurnell M., Ceral J., Ryska A., Mustangin M., Wong Y.P., Tan G.C., Solar M., Reincke M., Rainey W.E., Foo R.S., Takaoka Y., Murray S.A., Zennaro M.C., Beuschlein F., Ito A, & Brown M.J. (2023). Somatic mutations of CADM1 in aldosterone-producing adenomas and gap junction-dependent regulation of aldosterone production. Nature Genetics, 55(6), 1009-1021.
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3

Immunohistochemical Analysis of Mouse Tumor Xenografts

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Formalin-fixed tumor samples derived from mouse xenografts were processed and embedded in paraffin. Tissue sections were deparaffinized and antigen retrieval was achieved by use of heat-induced epitope retrieval with pH 6.0 citrate buffer (Sigma). Tissue sections were stained with hematoxylin and eosin (H&E), anti-TRPA1 antibody (Sigma), or anti-Cleaved Caspase-3 (Asp175) antibody (Cell Signaling). TRPA1 antibody was validated using sections from tumors that expressed TRPA1 shRNA vectors (Figure S1G). Immunostained slides were counterstained with hematoxylin (Sigma). The score of TRPA1 staining level was based on blinded analysis of epithelial staining by veterinary pathologist R.T.B.. The score of 2+ or greater was used for χ2 test. Images of tissue sections were captured at 4× magnification to create a merged image of the entire section and at 20× magnification for quantification of cleaved caspase-3-positive nuclei, using a Olympus VS120 Slide Scanner. Images of tumor sections stained with H&E, cleaved caspase-3, 4-HNE, and 8-OHdG are representative of at least two independent experiments. Relative apoptosis was quantified by determining the percentage of cleaved caspase-3-positive cells from more than five random fields, per section, from each tumor in the group, and normalized to the shGFP-expressing tumors treated with vehicle.
Takahashi N., Chen H.Y., Harris I.S., Stover D.G., Selfors L.M., Bronson R.T., Deraedt T., Cichowski K., Welm A.L., Mori Y., Mills G.B, & Brugge J.S. (2018). Cancer Cells Co-opt the Neuronal Redox-Sensing Channel TRPA1 to Promote Oxidative-Stress Tolerance. Cancer cell, 33(6), 985-1003.e7.
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4

Histological Analysis of Mouse Xenograft Tumors

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Formalin-fixed tumor samples derived from mouse xenografts were processed and embedded in paraffin. Tissue sections were deparaffinized and antigen retrieval was achieved by use of heat-induced epitope retrieval with pH 6.0 citrate buffer (Sigma). Tissue sections were stained with H&E, anti-ERBB2 antibody (Dako, A0485), or anti-S100A1antibody (Dako, Z031129-2). Immunostained slides were counterstained with hematoxylin (Sigma). Images of tissue sections were captured at 10x magnification to create a merged image of the entire section using Olympus VS120 Slide Scanner.
Amara S.N., Kuiken H.J., Selfors L.M., Butler T., Leung M.L., Leung C.T., Kuhn E.P., Kolarova T., Hage C., Ganesh K.S., Foster R., Rueda B.R., Aktipis A., Spellman P.T., Ince T.A., Navin N., Mills G.B., Bronson R.T., & Brugge J.S. (2020). Transient Commensal Clonal Interactions Can Drive Tumor Metastasis.
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