In vitro cytotoxicity was evaluated with a S35‐methionine release assay. Target cells were labeled with S35‐methionine (PerkinElmer, Waltham, MA, USA) overnight. Transduced PBMCs were then co‐cultured with target cells, 5 × 103 per well, for 5 h at indicated E:T ratios. Target cells alone were used for spontaneous release, and 50mM NaOH was added for total release. Plates were then centrifuged, 50 μL of the supernatant was transferred to a new plate, and 150 μL MicroScint 40 (PerkinElmer) was added to each well. S35‐methionine released into the medium due to specific tumor lysis was detected with a Micro β counter (PerkinElmer). Percent specific lysis was calculated as 100 × (sample release − spontaneous release)/(total release − spontaneous release). If sample release was lower than spontaneous release, it was considered that there was no killing. Assays were performed in triplicates.
Micro β counter
Manufactured by PerkinElmer
The Micro β counter is a compact and versatile instrument designed for the measurement of radioactivity in liquid samples. It utilizes a sensitive scintillation detector to accurately quantify the radioactive content in microplates, vials, or other small volume containers. The Micro β counter is a reliable tool for researchers and scientists working in fields that require the detection and analysis of radioactive materials.
Automatically generated - may contain errors
3 protocols using micro β counter
1
In vitro Cytotoxicity Assay for Transduced PBMCs
2
Measuring T Cell Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After restimulation, the cocultures were pulsed with 2µCi/µl of [3H] labelled thymidine (PerkinElmer, Waltham, MA, USA) and incubated for about 16h before harvesting. The radioactive thymidine incorporated into the DNA of proliferating T cells was determined by liquid scintillation counting using a micro-β counter (PerkinElmer).
3
Cell Proliferation Measurement Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were pulsed with [H3]thymidine (1μCi/well) (Perkin Elmer Life and Analytical Sciences, Boston, MA) for the last 18 hours of culture. Incorporation of [H3]thymidine was measured by β-scintillation counting (Micro β counter, Perkin Elmer), and the incorporation data were expressed as mean count per minute (cpm).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!