Tumor necrosis factor (tnf)

Manufactured by Genentech

TNF is a lab equipment product. It is a key protein involved in the immune system's inflammatory response.

Automatically generated - may contain errors

Lab products found in correlation

Lipopolysaccharides (lps), by InvivoGen (2 mentions) M csf, by Genentech (1 mentions) 5z 7 oxozeanol, by Bio-Techne (1 mentions) Il 1b af 401 na, by R&D Systems (1 mentions) Ciap1 2, by R&D Systems (1 mentions) Gne684, by Genentech (1 mentions) Emricasan, by Selleck Chemicals (1 mentions) Caspase 1, by Genentech (1 mentions) Ultrapure cholesterol, by Merck Group (1 mentions) Perfecta qpcr fastmix, by Quanta Biosciences (1 mentions) Anti cd15, by BD (1 mentions) Recombinant c5a, by Merck Group (1 mentions) 1 propanol, by Merck Group (1 mentions) Anti cd20, by Agilent Technologies (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Anti cd3, by Agilent Technologies (1 mentions) Anti cd68, by Agilent Technologies (1 mentions)

3 protocols using tumor necrosis factor (tnf)

Rat Bone Marrow-Derived Macrophage Culture

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Primary BMDMs were obtained from rats’ rear leg’s bone marrow and differentiated to macrophages for 6 days in DMEM medium supplemented with 10% heat-inactivated FBS, 1% non-essential amino acids, penicillin, streptomycin, 2 mM glutamine, and 25 ng/ml M-CSF at 37 °C with 5% CO₂.
Human recombinant TNF, Nec-1, zVAD, and BV6 were all synthesized at Genentech. LPS was purchased from InvivoGen, San Diego, CA, USA and TAK1 inhibitor 5z-7-oxozeanol from Bio-Techne Corporation, Minneapolis, MN, USA.

Stark K., Goncharov T., Varfolomeev E., Xie L., Ngu H., Peng I., Anderson K.R., Verschueren E., Choi M., Kirkpatrick D.S., Easton A., Webster J.D., McKenzie B.S., Vucic D, & Bingol B. (2021). Genetic inactivation of RIP1 kinase activity in rats protects against ischemic brain injury. Cell Death & Disease, 12(4), 379.

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Comprehensive Cell Death Pathway Analysis

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Human recombinant TNF, BV6, GNE684, and GSK583 were all synthesized at Genentech. Emricasan was purchased from Selleck Chemicals (S7775), zVAD from ABclonal and LPS from InvivoGen (tlrl-3peLPS). The primary antibodies used were directed against: RIP1 (610459, BD Biosciences), pRIP1 S166 (#31122, Cell Signaling Technology (CST)), RIP3 (#15828, CST), pRIP3 (#91702, CST), MLKL (#MABc604, Millipore), pMLKL (#37333, CST), FADD (#05-486, Millipore), RIP2 (#22763, Santa Cruz), XIAP (M044-3, MBL, 66800-1-Ig, Proteintech), c-IAP2 (Genentech), c-IAP1/2 (#3400, R&D), caspase-1(Genentech), caspase-3 (#9661, 9662, CST), caspase-7(#9491, CST), caspase-8 (#8592, 9429, 4927, CST), caspase-11(#14340, CST), GSDMD (#50928, CST), IL-1b (AF-401-NA, R&D), JNK(#9252, CST), pJNK (#4668, CST), p65(#8242, CST), p-p65(#3033, CST), IkBa (#9242, CST), p-IkBa (#2859, CST), p38 (#9212, CST), p-p38 (#9211, CST), ERK (#4695, CST), p-ERK (#4370, CST), actin (A3853, Sigma), GapDH (#2118, CST), HSP90 (#4877, CST).

Witt A., Goncharov T., Lee Y.M., Kist M., Dohse M., Eastham J., Dugger D., Newton K., Webster J.D, & Vucic D. (2023). XIAP deletion sensitizes mice to TNF-induced and RIP1-mediated death. Cell Death & Disease, 14(4), 262.

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Immune Profiling of Lepirudin-treated PBMCs

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PBMC were isolated from whole-blood anticoagulated with lepirudin (Refludan®, Celgene). Recombinant C5a, ultrapure cholesterol, 1-propanol were purchased from Sigma-Aldrich, and TNF from Genentech. The following reagents were used for qRT-PCR analyses: RNeasy Mini kit (Qiagen), DNase and High Capacity RNA-to-cDNA Kit (Applied Biosystems) and PerfeCTa® qPCR FastMix™ (Quanta Biosciences). Assays were from Applied Biosystems: GAPDH (Hs99999905_m1), NLRP3 (Hs00918082_m1), TNF (Hs00174128_m1), and IL-1β (Hs01555410_m1) and primers from SYBR as shown in Supplementary Table I. Antibodies used for immunohistochemistry: polyclonal rabbit anti-human C1q (A0136, Dako), normal rabbit IgG (Ab105-C, R&D systems), goat anti-mouse conjugated to Alexa 647 (Invitrogen), anti-C5b-9 (DIA-011, Antibodyshop), anti-CD68 (Dako Agilent M0814), anti-CD20 (Dako Agilent M0755), anti-CD3 (Dako Agilent A0452), anti-CD15 (BD Pharmingen 559045), control IgG2a (BD Pharmigen). Antibodies used for flow cytometry included anti-CD14 FITC (M5E2), anti-CD4 PerCP-CY 5.5 (RPA-T4), anti-CD8 BV605 (SK1) and anti-CD19 BV711 (SJ25C1) and were purchased from BD Pharmigen, and anti-CD3 APC (UCHT1) were from Invitrogen. Fluorescence-minus-one (FMO) was used as control.

Niyonzima N., Bakke S.S., Gregersen I., Holm S., Sandanger Ø., Orrem H.L., Sporsheim B., Ryan L., Kong X.Y., Dahl T.B., Skjelland M., Sørensen K.K., Rokstad A.M., Yndestad A., Latz E., Gullestad L., Andersen G.Ø., Damås J.K., Aukrust P., Mollnes T.E., Halvorsen B, & Espevik T. (2020). Cholesterol crystals use complement to increase NLRP3 signaling pathways in coronary and carotid atherosclerosis. EBioMedicine, 60, 102985.

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