Wildtype and ΔyciB cells were grown in LB containing low salt (75mM NaCl) before being transferred to LB with high salt (600mM NaCl) and grown for 6-7 generations before being fixed as described before 54 (link). Fixed cells were imaged on a Nikon Eclipse TE inverted fluorescence microscope with a 60X (NA 1.40) oil-immersion objective. Images were collected using the DS-Qi2 Mono Digital Microscope Camera and the NIS-Elements AR (Advanced Research) software (Nikon).
Eclipse te
Manufactured by Nikon
Sourced in Germany
The Nikon Eclipse TE is a high-performance inverted microscope designed for advanced cell and tissue culture applications. It features a stable and rigid frame, delivering reliable and consistent imaging performance. The Eclipse TE is equipped with a range of optical configurations, including phase contrast and fluorescence, enabling versatile imaging capabilities for various life science research and clinical applications.
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Lab products found in correlation
3 protocols using eclipse te
1
Imaging Salt Stress Response in Bacteria
2
Calcium Imaging of Hippocampal Neurons
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Hippocampal cells were loaded with Fluo-3 AM (1 μM in pluronic acid/DMSO, Molecular Probes, Eugene, OR, USA) for 30 min at 37°C and then washed twice with external solution, as described above, and incubated under control or experimental conditions for a maximal time of 30 min at 37°C. Neurons were mounted in a perfusion chamber that was placed on the stage of an inverted fluorescent microscope (Eclipse TE, Nikon), equipped with a CCD camera (SensiCam camera, PCO, Germany) and xenon excitation lamp. Cells were subsequently excited for 200 ms each 2 s intervals and recorded during 5 min, using a Lambda 10-2 filter wheel (Sutter Instruments) and regions of interest (ROI) were simultaneously selected on several neuronal somata on each plate (Ex:Em; 480:510 nm) recording more than 10 cells in each experiment. Finally, calcium transients, as defined by their TTX sensitivity (Gu et al., 1994 (link)), were acquired and analyzed off line with Axon Instruments Workbench 2.2 software.
3
Imaging Salt Stress Response in Bacteria
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