Kds101

In vivo microdialysis experiments used to assess brain interstitial fluid (ISF) Aβ levels from awake and freely moving APP/PS1 mice at the age of 3 months, 2 weeks after BCAS or sham operation, were developed with a modification of a previously described method^{26 (link)}. The guide cannula was stereotactically inserted into the hippocampus using the following coordinates: anterior-posterior −2.8 mm, medial-lateral ± 0.5 mm, dorsal-ventral −1.3 mm, from the bregma.
The probes used for in vivo microdialysis were equipped with 1000 kDa cut-off membrane, and connected to push (KDS101, Kd Scientific, Holliston, MA, USA) and pull pumps (ERP-10, Eicom, San Diego, CA, USA). The probes were manually inserted through the guide cannula into the target region. After probe insertion, mice were placed into cages designed to allow unrestricted movement of the animal without probe assembly tangling. To measure Aβ₄₀ and Aβ₄₂, microdialysis probes had a constant flow rate of 1.3 μl/min. Microdialysis samples were collected hourly using a refrigerated fraction collector.

To produce citral/CD-IC-NFs, each citral/CD-IC solution (citral/HPβCD-IC, citral/HPγCD-IC and citral/MβCD-IC) was separately loaded in syringes having a metallic needle of 0.4 mm inner diameter. Then, the syringe pump (KD Scientific, KDS-101, Holliston, MA, USA) was put on and pumped at a rate of 0.5 mL/h toward the collector covered with a piece of aluminum foil. The distance and electric field applied to the system (AU Series, Matsusada Precision Inc., Osaka, Japan) was arranged as 10–15 cm and 15–20 kV, respectively. The electrospinning was carried out in an enclosed Plexiglas box at 25°C and 18% relative humidity. The electrospun webs of citral/CD-IC-NFs were kept in a refrigerator (4°C) prior to analysis. For a comparative time-dependent stability study, a polymeric system without CD-IC was also tested. That is, PVA (10% w/v with respect to solvent) nanofibers with only citral (10% w/w with respect to polymer) (citral/PVA-NF)) were also produced from citral/PVA aqueous solution by electrospinning.

Different amounts of GO (1–7 mg) were ultrasonically dispersed in DMF/acetone (3/2 w/w, 9 g) for 2 h. PVDF was dissolved in the resulting suspensions to give a polymer/solvent weight ratio of 1:9 (10%, w/w). Each mixture was stirred for 12 h to form a homogenous solution. PHBV was dissolved in trichloromethane at 60 °C to prepare PHBV solution (8%, w/w). In a custom-made electrospinning set-up (Fig. S1), a polymer solution was charged into a syringe that was subjected to a DC voltage if up to 18 kV by DC power supply (JG50-1, Shanghai Shenfa Detection Instrument, China). The syringe was placed in a microsyringe pump (KDS101, KD Scientific, USA) and the spinning solution was delivered to the blunt needle tip at a flow rate of 1 mL h⁻¹ at a fixed collection distance of 15 cm between the tip of the syringe and roller collector. The relative humidity was controlled below 30%.

To obtain uniform and bead-free nanofibrous web, we have prepared homogeneous PAN, Nylon 66 and PSU in DMF, formic acid and DMAc:acetone (9:1, v-v) at 13%, 8% and 32% (w/v, with respect to solvent) polymer concentrations, respectively. After that, the well-stirred solution was loaded in 3 mL or 10 mL syringe fitted with a metallic needle of 0.4 mm inner diameter. The syringe was located horizontally on the syringe pump (KD Scientific, KDS 101) with a 0.5–1 mL/h flow rate. A high voltage; 10–15 kV was applied by high voltage power supply (Matsusada, AU Series) to the tip of the needle to initiate the electrospinning jet movement through the stationary plate metal collector which is covered with aluminum foil and positioned at 10 cm from the end of the tip. The electrospinning process was carried out at ∼25 °C and 22% relative humidity in an enclosed Plexi-glass chamber. The all collected nanofibers/nanowebs were place in the hood for overnight to remove the residual solvent.