THA (≥98%) was purchased from Meilun Biotech Co. Ltd (Dalian, People’s Republic of China). ε-Caprolactone (ε-CL) was purchased from Alfa Aesar (Ward Hill, MA, USA). MPEG (Mn =2,000), stannous octoate [Sn(Oct)2], and MTT were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Dimethyl sulfoxide (DMSO), methyl alcohol, and acetonitrile (HPLC grade) were supplied by Kelong Co. Ltd. (Chengdu, People’s Republic of China). A TNF-α ELISA kit was purchased from Beijing Cheng Lin Biological Technology Co. Ltd. (Beijing, People’s Republic of China). Ki-67, CD31, and VEGF polyclonal antibody were purchased from Bioworld Technology Co. Ltd. (Nanjing, People’s Republic of China).
Ki 67
Manufactured by Bioworld Technology
Sourced in United States, China
Ki-67 is a protein marker that is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent in resting cells (G0). It is commonly used as a marker of cellular proliferation in immunohistochemical and flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Stannous octoate sn oct 2, by Merck Group (2 mentions)
Mpeg mn 2000, by Merck Group (2 mentions)
ε caprolactone, by Thermo Fisher Scientific (2 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
γ h2ax, by Bioworld Technology (1 mentions)
Cd 31 polyclonal antibodies, by Bioworld Technology (1 mentions)
3 3 diaminobenzidine (dab), by Beyotime (1 mentions)
Hrp conjugated anti rabbit antibody, by ZSGB-BIO (1 mentions)
5 protocols using ki 67
1
Synthesis and Characterization of THA Loaded PLGA Nanoparticles
2
Immunohistochemical Analysis of Bmi-1, p16, p53, and Ki67
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Serial paraffin sections were generated for antigen retrieval, steamed for 20 min in sodium citrate buffer (10 mM sodium citrate acid, 0.05% Tween-20, pH 6.0) followed by blocking of endogenous peroxidase (3% H2O2) and preincubation with serum (Jin et al., 2014 (link); Xie et al., 2015 (link)). Primary antibodies were against Bmi-1 (#5856, Cell Signaling Technology, United States), p16 (#ab211542, Abcam, Cambridge, MA, United States), p53 (#2524, Cell Signaling Technology, United States), and Ki67 (#BS1679, Bioworld Technology Inc., MN, United States). Biotin-labeled second antibody was used. Nuclei were stained with hematoxylin.
3
Polymer Synthesis and Cellular Assays
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Ola with 99.8% purity was purchased from Meilun Co., Ltd (Dalian, China). MPEG (Mn =2,000) was purchased from Sigma-Aldrich (St Louis, MO, USA), ε-caprolactone from Alfa Aesar (Reston, VA, USA), and stannous octoate (Sn(Oct)2) purchased from Sigma-Aldrich. Dimethylsulfoxide (DMSO), anhydrous ethanol, and methanol (HPLC grade) were purchased from KeLong Co., Ltd (Chengdu, China). γ-H2AX, CC3, Ki-67, and CD-31 polyclonal antibodies were purchased from Bioworld Technology Co. Ltd. (Nanjing, China).
4
Tumor Proliferation Assay by IHC
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Tumors from subcutaneous implantation assay were fixed in 4% paraformaldehyde, and then were dehydrated, embedded in paraffin, and cut. Consecutive 4 μm thick sections were analyzed by immunohistochemistry using antibodies against Ki-67 (Bioworld Technology, Louis Park, MN, USA). Antigen retrieval was performed using citrate buffer at pH 6.0. DAB (Beyotime Institute of Biotechnology, Jiangsu, China) systems were used for detection.
5
Immunohistochemical analysis of proliferation and differentiation markers
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Tissue slides were deparaffinized, rehydrated and boiled in citrate buffer. The tissue sections were incubated with primary Ki67 (1:100, Bioworld Technology, China), CEA (1:100, Bioworld Technology, China), CK20 (1:100, Bioworld Technology, China) and CK7 (1:100, Proteintech, China) antibodies overnight at 4 °C. Then, the sections were incubated with an HRP-conjugated anti-rabbit antibody (1:1000, ZSGB-BIO, China) for 30 min at 37 °C, stained with DAB, and counterstained with haematoxylin.
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