In the experiment, 33 OSCC patients treated in the Affiliated Hospital of Taishan Medical University (Taian, China) from January 2016 to January 2018 were selected as the experimental group, including 18 males and 15 females, aged 45 years on average, while 33 healthy subjects were selected as the control group, including 17 males and 16 females, aged 45 years on average. The experimental scheme was discussed and approved by the Academic Committee, and agreed by the family members.
This study was approved by the Ethics Committee of Affiliated Hospital of Taishan Medical University. Patients who participated in this research had complete clinical data. The signed informed consents were obtained from the patients or the guardians.
The following experimental reagents were used: Fluorescence quantitative polymerase chain reaction (PCR) reagent and ribonucleic acid (RNA) extraction reagent were purchased from Takara and animal cell protein extraction kit was purchased from Thermo Fisher Scientific, Inc. Streptomycin-peroxidase (S-P) kit and corresponding antibodies were purchased from Thermo Fisher Scientific, Inc. Genome extraction kit was purchased from AXYGEN and other reagents and consumables were purchased from Sangon Biotech Co., Ltd.
This study was approved by the Ethics Committee of Affiliated Hospital of Taishan Medical University. Patients who participated in this research had complete clinical data. The signed informed consents were obtained from the patients or the guardians.
The following experimental reagents were used: Fluorescence quantitative polymerase chain reaction (PCR) reagent and ribonucleic acid (RNA) extraction reagent were purchased from Takara and animal cell protein extraction kit was purchased from Thermo Fisher Scientific, Inc. Streptomycin-peroxidase (S-P) kit and corresponding antibodies were purchased from Thermo Fisher Scientific, Inc. Genome extraction kit was purchased from AXYGEN and other reagents and consumables were purchased from Sangon Biotech Co., Ltd.