RA (purity ≥98%) and Alamar Blue® (a solution of resazurin fluorescence dye) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Trypsin-EDTA solution, penicillin/streptomycin, and Dulbecco’s phosphate-buffered saline (DPBS) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA). An Annexin V-FITC Apoptosis Detection Kit Plus (cat. no. 68FT-Ann VP-S) was purchased from RayBiotech (Norcross, GA, USA). Propidium Iodide Flow Cytometry Kit (cat. No. ab139418) was purchased from Abcam (Cambridge, MA, USA). A DNA-free™ kit (cat. no. AM1907) was purchased from Life Technologies, Inc. (Thermo Fisher Scientific, Inc., Waltham, MA, USA). An iScript™ cDNA Synthesis kit (cat. no. 170–8890), SsoAdvanced™ Universal SYBR® Green Supermix, and the Human Apoptosis PCR array (SAB Target List) H96 were purchased from Bio-Rad Laboratories (Hercules, CA, USA). Dulbecco’s modified Eagle’s medium (DMEM) and heat-inactivated fetal bovine serum (FBS) were purchased from VWR International (Radnor, PA, USA). Cell culture flasks were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Cell culture plates were purchased from Thermo USA Scientific (Ocala, FL, USA).
Heat inactivated fetal bovine serum
Manufactured by Avantor
Sourced in United States
Heat-inactivated fetal bovine serum (FBS) is a commonly used laboratory reagent. It is obtained from the blood of bovine fetuses and undergoes a heat-inactivation process to deactivate any potential contaminants. FBS is a rich source of proteins, growth factors, and other essential nutrients required for the cultivation and maintenance of various cell lines in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Ssoadvanced universal sybr green supermix, by Bio-Rad (3 mentions)
Penicillin streptomycin, by American Type Culture Collection (3 mentions)
Dulbecco s modified eagle s medium dmem, by Avantor (3 mentions)
Alamar blue, by Merck Group (3 mentions)
Trypsin edta solution, by American Type Culture Collection (3 mentions)
Cell culture flasks, by Santa Cruz Biotechnology (3 mentions)
Dulbecco s phosphate buffered saline, by American Type Culture Collection (2 mentions)
Human apoptosis pcr array sab target list h96, by Bio-Rad (2 mentions)
Phosphate buffered saline (pbs), by American Type Culture Collection (1 mentions)
3 protocols using heat inactivated fetal bovine serum
1
Apoptosis Evaluation in Cell Cultures
2
Apoptosis Pathway Analysis in Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TQ (purity ≥ 99%, cat # MKCC0600) and Alamar Blue® (a resazurin fluorescence dye solution) were acquired from Sigma-Aldrich (St. Louis, MO, USA). Trypsin-EDTA solution, penicillin/streptomycin, and phosphate-buffered saline (PBS) were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). An Annexin VFITC Apoptosis Detection Kit Plus (cat. no. 68FT-Ann VP-S) was purchased from RayBiotech (Norcross, GA, USA). Propidium Iodide Flow Cytometry Kit (cat. no. ab139418) was purchased from Abcam (Cambridge, MA, USA). A DNA-free™ kit (cat. no. AM1907) was purchased from Life Technologies, Inc. (Thermo Fisher Scientific, Inc., Waltham, MA, USA). An iScript™ cDNA Synthesis kit (cat. no. 170-8890), SsoAdvanced™ Universal SYBR® Green Supermix, and the Human Apoptosis PCR array (Cat. no. 10034106) H96 were purchased from BioRad Laboratories (Hercules, CA, USA). Dulbecco’s modified Eagle’s medium (DMEM) and heat-inactivated fetal bovine serum (FBS) were purchased from VWR International (Radnor, PA, USA). Cell culture flasks were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Cell culture plates were purchased from Thermo USA Scientific (Ocala, FL, USA).
3
Apoptosis Evaluation in Cell Cultures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RA (purity ≥98%) and Alamar Blue® (a solution of resazurin fluorescence dye) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Trypsin-EDTA solution, penicillin/streptomycin, and Dulbecco’s phosphate-buffered saline (DPBS) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA). An Annexin V-FITC Apoptosis Detection Kit Plus (cat. no. 68FT-Ann VP-S) was purchased from RayBiotech (Norcross, GA, USA). Propidium Iodide Flow Cytometry Kit (cat. No. ab139418) was purchased from Abcam (Cambridge, MA, USA). A DNA-free™ kit (cat. no. AM1907) was purchased from Life Technologies, Inc. (Thermo Fisher Scientific, Inc., Waltham, MA, USA). An iScript™ cDNA Synthesis kit (cat. no. 170–8890), SsoAdvanced™ Universal SYBR® Green Supermix, and the Human Apoptosis PCR array (SAB Target List) H96 were purchased from Bio-Rad Laboratories (Hercules, CA, USA). Dulbecco’s modified Eagle’s medium (DMEM) and heat-inactivated fetal bovine serum (FBS) were purchased from VWR International (Radnor, PA, USA). Cell culture flasks were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Cell culture plates were purchased from Thermo USA Scientific (Ocala, FL, USA).
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